Supplementation of freezing media with alpha lipoic acid preserves the structural and functional characteristics of sperm against cryodamage in infertile men with asthenoteratozoospermia

Asa, Elham; Ahmadi, Rahim; Mahmoodi, Minoo; Mohammadniya, Abdolreza

doi:10.1016/j.cryobiol.2020.07.001

Cited by 11 publications

(10 citation statements)

References 47 publications

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“…In addition, the number of TUNEL‐negative cells was significantly higher in two groups NAC and ALA compared to the control group, but their combination did not show any significant difference in this factor. The positive effect of NAC and ALA Separately on sperm quality has been consistently reported in previous studies (Asa et al, 2020; Jannatifar et al, 2021). In the study by Jannatifar et al, the 1 mg/ml of NAC improved sperm motility, sperm viability and reduced DNA damage (Jannatifar et al, 2021).…”

Section: Discussionsupporting

confidence: 80%

“…In the study by Jannatifar et al, the 1 mg/ml of NAC improved sperm motility, sperm viability and reduced DNA damage (Jannatifar et al, 2021). In previously study, it has been observed that ALA in cryopreservation process can be significantly increases the quality of sperm parameters such as motility and viability of sperm and also reduces DNA fragmentation (Asa et al, 2020). We have shown for the first time that adding antioxidants to the freezing medium can significantly increases MMP levels.…”

Section: Discussionmentioning

confidence: 98%

“…ALA and its reduced form, dihydrolipoic acid (DHLA), are powerful antioxidant molecules that can act as a scavenger of the ROS and can regenerate other antioxidant molecules (Dong et al, 2019). The result of studies have indicated that ALA with effect on Krebs cycle and production of adenosine triphosphate (ATP) can effective on sperm motility and viability (Asa et al, 2020; Canepa et al, 2018; Haghighian et al, 2015). Based on recent research on well‐known antioxidant capacity of NAC and ALA, this study was done to investigate the effect of ALA, NAC and combination of NAC + ALA on sperm quality against cryo damage during cryopreservation process in asthenozoospermia men.…”

Section: Introductionmentioning

confidence: 99%

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N‐acetyl‐ l ‐cysteine and alpha lipoic acid are protective supplement on human sperm parameters in cryopreservation of asthenoteratozoospermia patients

et al. 2022

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The aim of this study is to evaluate the beneficial effects of N-acetyl-cysteine (NAC), alpha lipoic acid (ALA) and combination of NAC + ALA supplement in freezing medium on Sperm structural and functional in asthenoteratozoospermia patients.Thirty freshly ejaculated semen samples were cryopreserved with sperm freezing medium (SFM) as control group and three group that SFM supplemented with NAC, ALA and their combination NAC+ ALA. The sperm samples were analysed according to WHO. Mitochondrial membrane potential (MMP), acrosome reaction (AR), antioxidant enzymes and DNA fragmentation were assessed using by Rhodamine123, PSA-FITC ELISA and TUNEL staining respectively. Expression level of NRF2 was assessed by real-time PCR assay. NAC and ALA alone significantly improved sperm motility, viability and DNA fragmentation (p < 0.05). MMP increased in NAC and ALA separately (p < 0.05). While did not affect the amount of sperm morphology and AR (p > 0.05). Antioxidant enzymes significantly difference in NAC and ALA groups (p < 0.05). In addition, NAC and ALA groups showed a significantly higher expression of NRF2 gene compared with other groups (p < 0.05). Our results revealed that the ALA and NAC supplements had a protective effect in cryopreservation process on the structural and functional characteristics of sperm.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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N‐acetyl‐ l ‐cysteine and alpha lipoic acid are protective supplement on human sperm parameters in cryopreservation of asthenoteratozoospermia patients

et al. 2022

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“…In order to decrease ROS production, many studies suggested the beneficial effect of administering some antioxidant compounds, which are the main defence factors against OS induced by ROS [ 5 , 10 , 11 , 12 , 13 ]. Recently, our research group demonstrated that an in vitro treatment with myo-inositol is effective in protecting sperm against oxidative damage to DNA [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Oxidative Stress Measurement in Frozen/Thawed Human Sperm: The Protective Role of an In Vitro Treatment with Myo-Inositol

et al. 2021

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Despite its widespread use, sperm cryopreservation induces serious detrimental alterations in sperm function; indeed, it is commonly associated with decreased sperm viability and motility, and DNA fragmentation. Mechanisms of human sperm cryodamage are thought to be multifactorial, but oxidative stress seems to have a prominent role. A huge amount of data supported the cryoprotective effect of different antioxidants able to minimize the detrimental effects of reactive oxygen species (ROS) and improve the quality of spermatozoa. Among others, myo-inositol is one of the most powerful and has been reported to be effective in improving sperm quality and motility when used both in vivo and in vitro. This study aimed to determine the in vitro impact of myo-inositol in ameliorating sperm oxidative status during sperm cryopreservation. In particular, we demonstrated a significant improvement of sperm parameters (vitality and motility) when myo-inositol was added after sperm thawing (p < 0.05). Moreover, we showed that myo-inositol induces a significant increase in oxygen consumption, the main index of oxidative phosphorylation efficiency and ATP production. Finally, by means of 2D-electrophoresis, we demonstrated a significant decrease in the level of carbonyl groups, the main structural changes occurring in conditions of oxidative stress (p < 0.05). In conclusion, the sperm cryopreservation procedure we developed, assuring the reduction of ROS-induced sperm modifications, may improve the in vitro procedure currently used in ART laboratory for sperm cryostorage.

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“…In general, low temperatures during cryopreservation process can negatively affect the structure and functionality of spermatozoa, which caused membrane and mitochondrial disruption, loss of sperm motility and viability, destabilization of plasmatic membrane lipid composition or premature capacitation known as cryocapacitation. Capacitation is a maturation process of spermatozoa, which involves a series of complex changes on the level of plasmatic membrane before acrosome reaction (AR) and fusion with oocyte (Yeste, 2016;Chauhan et al, 2018;Asa et al, 2020). The term cryocapacitation refers to cryoinduced membrane reorganization and loss of polyunsaturated fatty acids (PUFA's) and degradation of cholesterol associated with other typical capacitation hallmarks like higher concentration of intracellular calcium and protein phosphorylation (Ledesma et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Cryocapacitation and Its Association With Oxidative Features in Cryopreserved Bovine Spermatozoa

Benko

Ďuračka²,

Lukáč³

et al. 2022

J microb biotech food sci

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The goal of our research was to evaluate the impact of cryopreservation on the antioxidant activity, lipid peroxidation and protein carbonylation as well as its connection with the progress of cryocapacitation in bovine spermatozoa. As biological material we used semen obtained from 20 sexually mature Holstein bulls. Each ejaculate was divided into three equal aliquots as follows: the first part or control (CTRL) was incubated in physiological saline solution while the second part (CAP) was incubated in a capacitation medium at specific conditions. The third part of each sample was cryopreserved (CRYO) and stored in liquid nitrogen at -196°C for further analysis. The motility of spermatozoa was assessed with CASA (computer assisted sperm analysis), while the capacitation status was evaluated by the chlortetracycline (CTC) fluorescent staining. Total antioxidant capacity (TAC) and the level of lipid peroxidation (LPO) was measured with a combined spectro-fluoro-luminometer. The presence of protein oxidation was detected by the traditional DNPH (2,4-dinitrophenylhydrazine) method and evaluated spectrophotometrically. Based on our data, the motility was significantly decreased (P<0.0001; P<0.01) in the CRYO group against CAP and CTRL. There was a significant increase (P<0.01; P<0.05) of acrosome-reacted spermatozoa (AR-pattern) in the CRYO group when compared to CTRL and CAP, while TAC was statistically decreased (P<0.05) between the CRYO and CAP experimental group. In the case of protein oxidation and LPO, both parameters were significantly higher (P<0.0001) in the CRYO group when compared to CAP or CTRL. In summary, cryopreservation induces capacitation-like changes and promotes oxidative damage in frozen-thawed bovine spermatozoa.

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Supplementation of freezing media with alpha lipoic acid preserves the structural and functional characteristics of sperm against cryodamage in infertile men with asthenoteratozoospermia

Cited by 11 publications

References 47 publications

N‐acetyl‐ l ‐cysteine and alpha lipoic acid are protective supplement on human sperm parameters in cryopreservation of asthenoteratozoospermia patients

N‐acetyl‐ l ‐cysteine and alpha lipoic acid are protective supplement on human sperm parameters in cryopreservation of asthenoteratozoospermia patients

Oxidative Stress Measurement in Frozen/Thawed Human Sperm: The Protective Role of an In Vitro Treatment with Myo-Inositol

Cryocapacitation and Its Association With Oxidative Features in Cryopreserved Bovine Spermatozoa

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