Supplementation of extender with coenzyme Q10 improves the function and fertility potential of rooster spermatozoa after cryopreservation

Masoudi, Reza; Sharafi, Mohsen; Shahneh, Ahmad Zare; Kohram, Hamid; Nejati-Amiri, E.; Karimi, Hamideh; Khodaei-Motlagh, M.; Shahverdi, Abdolhossein

doi:10.1016/j.anireprosci.2018.09.019

Cited by 36 publications

(13 citation statements)

References 63 publications

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“…It has been suggested that the best results are obtained when glycerol is used with the in-straw method ( Blesbois et al, 2007 ). The commonly used frozen semen straw are mini straw (0.25 mL) and medium straw (0.5 mL), which are the same length and different diameters ( Masoudi et al, 2018 ; Thélie et al, 2019 ). Their different surface-to-volume ratios may exert effect on post-thaw recovery of sperm motility and thus fertility.…”

Section: Introductionmentioning

confidence: 99%

Effect of glycerol concentration, glycerol removal method, and straw type on the quality and fertility of frozen chicken semen

Zong¹,

Sun²,

Li³

et al. 2022

Poultry Science

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Section: Introductionmentioning

confidence: 99%

Effect of glycerol concentration, glycerol removal method, and straw type on the quality and fertility of frozen chicken semen

Zong¹,

Sun²,

Li³

et al. 2022

Poultry Science

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“…Lin and Liu (2021) reported that sperm motility was significantly improved in the group with Co-Q10 supplementation. Masoudi (2018) also reported that supplementation with Co-Q10 resulted in greater sperm motility and mitochondrial activity. Sperm motility is a key factor in determining fertilization and plays a key role in whether sperm can combine with an egg to fertilize it.…”

Section: Resultsmentioning

confidence: 94%

The Effect of the Addition of Co-Q10 on the Preservation of Boar Semen at 17°C

Zhang

Liu

et al. 2022

IJAR

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Background: With the increasing application of artificial insemination technology in boars, breeding efficiency is largely determined by the quality of semen preservation. With the prolonged storage of semen at room temperature in vitro, excessive reactive oxygen species (ROS) damage organelles and plasma membranes and ultimately lead to a decline in semen quality. Methods: In order to improve the preservation of semen at 17°C, this experiment investigated the effect of different concentrations (0, 10, 15, 25, 35 µg/mL) of coenzyme Q10 (Co-Q10) in Modena dilution on the quality of boar semen during storage at 17°C. The sperm motility, mitochondrial activity, total antioxidant (T-AOC), superoxide dismutase (SOD) and malondialdehyde (MDA) were assessed at 17°C at different times (0, 1, 2, 3, 4, 5 d). Result: The results showed that the addition of Co-Q10 has a positive effect during the preservation of boar semen at 17°C. On the 5th day, the sperm motility of the 25 µg/mL Co-Q10 group was 73.80% and the mitochondrial activity was 82.94%, which was significantly higher (P less than 0.05) than that of other groups. In terms of antioxidant levels, the MDA content of the 25 µg/ml and 35 µg/ml Co-Q10 groups on the 5th day was significantly lower (P less than 0.05) than that of the other groups. At the same time, the 25 µg/ml group could effectively enhance the activity of antioxidant enzymes and the SOD and T-AOC were 17.33 U/mL and 3.17 U/mL on the 5th day significantly higher than the other groups (P less than 0.05). The results showed that the most appropriate concentration of Co-Q10 when storing boar semen at 17°C was 25 µg/ml.

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“…The present study showed that positive effects of MitoQ were observed in the acrosome’s integrity with respect to thawed sperm. Another study confirmed that coenzyme Q10 (CoQ10, the active component of MitoQ) contributes to stabilizing the acrosome membrane of rooster semen during cryopreservation [ 31 ].…”

Section: Discussionmentioning

confidence: 97%

Does Antioxidant Mitoquinone (MitoQ) Ameliorate Oxidative Stress in Frozen–Thawed Rooster Sperm?

Sun

et al. 2022

Animals

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In this study, we aimed to determine the benefit of mitoquinone (MitoQ) in rooster semen extenders on sperm quality, motility parameters, antioxidant capacities, and apoptotic changes in post-thawed rooster semen. A total of 85 ejaculates from 18 roosters were collected and then divided into five equal aliquots and cryopreserved in extenders with 1.0% soy lecithin nanoparticles that contained various concentrations of MitoQ (0 nM (M0), 50 nM (M50), 100 nM (M100), 150 nM (M150), and 200 nM (M200)). By using a computer-assisted semen analyzer, sperm motility parameters were assessed after freeze thawing. The M150 group had significantly higher percentages of total motility, progressive motility, viability, acrosome membrane integrity, and mitochondrial activity than the other groups (p < 0.05). Compared to other groups, M100 and M150 groups produced a higher percentage of plasma membrane integrity and ATP contents (p < 0.05). Additionally, the lowest levels of ROS and MDA in spermatozoa were observed in M150 group (p < 0.05), whereas the highest levels of ROS and MDA were observed in sperm in the controls or the M200 group (p < 0.05). Significantly higher values of SOD, GPx, and Cas-3 were found in the M150 group compared to other groups (p < 0.05). Overall, these results demonstrate that MitoQ at 150 nM not only ameliorates post-thawed sperm quality and motility parameters by restoring ATP levels and preventing membrane damage, but also improves redox balance and antiapoptotic activities.

show abstract

Supplementation of extender with coenzyme Q10 improves the function and fertility potential of rooster spermatozoa after cryopreservation

Cited by 36 publications

References 63 publications

Effect of glycerol concentration, glycerol removal method, and straw type on the quality and fertility of frozen chicken semen

Effect of glycerol concentration, glycerol removal method, and straw type on the quality and fertility of frozen chicken semen

The Effect of the Addition of Co-Q10 on the Preservation of Boar Semen at 17°C

Does Antioxidant Mitoquinone (MitoQ) Ameliorate Oxidative Stress in Frozen–Thawed Rooster Sperm?

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