Supplementation of Bacillus subtilis DSM 32540 improves performance and intestinal health of weaned pigs fed diets containing different fiber sources

Júnior, Dante Teixeira Valente; Rodrigues, Gustavo de Amorim; Soares, Marcos Henrique; Silva, Caroline Brito; Frank, Elisa Oliveira; González-Vega, J. C.; Htoo, J. K.; Brand, Henrique Gastmann; Silva, Bruno Alexander Nunes; Saraiva, Alysson

doi:10.1016/j.livsci.2023.105202

Cited by 3 publications

(2 citation statements)

References 58 publications

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“…Five cross-sections (5 μm thickness each) were placed per slide and stained with hematoxylin and eosin. The sections were semi-serial using 1 in 10 cuts 51 . For morphological readings of villus height and crypt depth in the duodenum, jejunum, and ileum, an EVOS™ M5000 Cell Imaging System optical microscope (Invitrogen, Thermo Fisher Scientific) with a 10-objective lens was used.…”

Section: Intestinal Morphology Peyer's Patches and Goblet Cellsmentioning

confidence: 99%

Autolyzed yeast and sodium butyrate supplemented alone to diets promoted improvements in performance, intestinal health and nutrient transporter in weaned piglets

Correia,

Genova,

Kim

et al. 2024

Sci Rep

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This study investigated the effects of supplemental nucleotides, autolyzed yeast (Saccharomyces cerevisiae), and sodium butyrate in diets for nursery pigs on growth performance, diarrhea incidence, blood profile, intestinal morphology, mRNA expression of nutrient transporters, inflammatory markers, antioxidant profile, and tight junction proteins in the small intestine. One hundred eighty 21-day-old pigs (5.17 ± 0.57 kg) were assigned in a randomized block design to 1 of 4 dietary treatments: (1) CON: control, basal diet, (2) NUC: CON + nucleotides, (3) YSC: CON + lysed yeast S. cerevisiae, (4) ASB: CON + acidifier sodium butyrate. Pigs were fed for 24 days, phase 1 (21–32 days) and 2 (32–45 days). During phase 1, YSC and ASB improved average daily gain (ADG) and feed conversion (FC) compared with CON. At the overall period, ASB improved ADG and YSC improved FC compared with CON. The NUC diet did not affect growth performance. The ASB increased ileal villus height compared to CON. The YSC and ASB reduced the number of Peyer’s patches in the ileum compared with CON. The YSC increased mRNA expression of nutrient transporters (SMCT2, MCT1, and PepT1), tight junction proteins (OCL and ZO-1), antioxidants (GPX), and IL1-β in the jejunum compared with CON. The ASB increased mRNA expression of nutrient transporters (SGLT1 and MCT1), tight junction proteins (OCL and ZO-1), and antioxidants (GPX and SOD) compared with CON. In conclusion, autolyzed yeast and sodium butyrate promoted growth performance by improving the integrity of the intestinal barrier, the mRNA expression of nutrient transporters, and antioxidant enzymes in the jejunum of nursery pigs whereas supplementation of nucleotides did not show such effects.

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Section: Intestinal Morphology Peyer's Patches and Goblet Cellsmentioning

confidence: 99%

Autolyzed yeast and sodium butyrate supplemented alone to diets promoted improvements in performance, intestinal health and nutrient transporter in weaned piglets

Correia,

Genova,

Kim

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Five cross-sections (5 µm thickness each) were placed per slide and stained with hematoxylin and eosin. The sections were semi-serial using 1 in 10 cuts 51 . For morphological readings of villus height and crypt depth in the duodenum, jejunum, and ileum, an EVOS™ M5000 Cell Imaging System optical microscope (Invitrogen, Thermo Fisher Scienti c) with a 10-objective lens was used.…”

Section: Sample Collectionmentioning

confidence: 99%

Autolyzed yeast and sodium butyrate supplemented alone to diets promoted improvements in performance, intestinal health and nutrient transporter in weaned piglets

Correia¹,

Genova¹,

Kim

et al. 2024

Preprint

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This study investigated the effects of supplemental nucleotides, autolyzed yeast (Saccharomyces cerevisiae), and sodium butyrate in diets for nursery pigs on growth performance, diarrhea incidence, blood profile, intestinal morphology, mRNA expression of nutrient transporters, inflammatory markers, antioxidant profile, and tight junction proteins in the small intestine. One hundred eighty 21-d-old pigs (5.17 ± 0.57 kg) were assigned in a randomized block design to 1 of 4 dietary treatments: (1) CON: control, basal diet, (2) NUC: CON + nucleotides, (3) YSC: CON + lysed yeast S. cerevisiae, (4) ASB: CON + acidifier sodium butyrate. Pigs were fed for 24 d, phase 1 (21 to 32 d) and 2 (32 to 45 d). During phase 1, YSC and ASB improved average daily gain (ADG) and feed conversion (FC) compared with CON. At the overall period, ASB improved ADG and YSC improved FC compared with CON. The NUC diet did not affect growth performance. The ASB increased ileal villus height compared to CON. The YSC and ASB reduced the number of Peyer’s patches in the ileum compared with CON. The YSC increased mRNA expression of nutrient transporters (SMCT2, MCT1, and PepT1), tight junction proteins (OCL and ZO-1), antioxidants (GPX), and IL1-β in the jejunum compared with CON. The ASB increased mRNA expression of nutrient transporters (SGLT1 and MCT1), tight junction proteins (OCL and ZO-1), and antioxidants (GPX and SOD) compared with CON. In conclusion, autolyzed yeast and sodium butyrate promoted growth performance by improving the integrity of the intestinal barrier, the mRNA expression of nutrient transporters, and antioxidant enzymes in the jejunum of nursery pigs whereas supplementation of nucleotides did not show such effects.

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Bacillus spp. as potential probiotics: promoting piglet growth by improving intestinal health

Tang,

Zeng,

Xiong

et al. 2024

Front. Vet. Sci.

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The application of Bacillus spp. as probiotics in the swine industry, particularly for piglet production, has garnered significant attention in recent years. This review aimed to summarized the role and mechanisms of Bacillus spp. in promoting growth and maintaining gut health in piglets. Bacillus spp. can enhance intestinal barrier function by promoting the proliferation and repair of intestinal epithelial cells and increasing mucosal barrier integrity, thereby reducing the risk of pathogenic microbial invasion. Additionally, Bacillus spp. can activate the intestinal immune system of piglets, thereby enhancing the body’s resistance to diseases. Moreover, Bacillus spp. can optimize the gut microbial community structure, enhance the activity of beneficial bacteria such as Lactobacillus, and inhibit the growth of harmful bacteria such as Escherichia coli, ultimately promoting piglet growth performance and improving feed efficiency. Bacillus spp. has advantages as well as challenges as an animal probiotic, and safety evaluation should be conducted when using the newly isolated Bacillus spp. This review provides a scientific basis for the application of Bacillus spp. in modern piglet production, highlighting their potential in improving the efficiency of livestock production and animal welfare.

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Supplementation of Bacillus subtilis DSM 32540 improves performance and intestinal health of weaned pigs fed diets containing different fiber sources

Cited by 3 publications

References 58 publications

Autolyzed yeast and sodium butyrate supplemented alone to diets promoted improvements in performance, intestinal health and nutrient transporter in weaned piglets

Autolyzed yeast and sodium butyrate supplemented alone to diets promoted improvements in performance, intestinal health and nutrient transporter in weaned piglets

Autolyzed yeast and sodium butyrate supplemented alone to diets promoted improvements in performance, intestinal health and nutrient transporter in weaned piglets

Bacillus spp. as potential probiotics: promoting piglet growth by improving intestinal health

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