Supplementation of astaxanthin and vitamin E in feed on the development of gonads white shrimp broodstock Litopenaeus vannamei Boone 1931

Abstract

The quality of white shrimp Litopenaeus vannamei broodstock can be improved through the addition of astaxanthin and vitamin E in the diet. This study aimed to determine the effect of administration of astaxanthin and vitamin E with different doses in the feed on the maturity gonad of prospective Pacific white shrimp broodstock. Supplementation of 0 mg/kg feed astaxanthin + 0 mg/kg feed vitamin E (control/A), 500 mg/kg feed astaxanthin (B), 350 mg/kg feed vitamin E (C), 500 mg/kg feed astaxanthin and 350 mg/kg feed vitamin E (D), and 250 mg/kg feed astaxanthin and 175 mg/kg feed vitamin E (E) were applied in feed formulation. Shrimp was fed 2% of body weight three times daily at 06.00 am, 13.00 pm, and 20.00 pm. The result showed that the optimum dose for survival, specific growth rate and maturity level of Pacific white shrimp broodstock was obtained in the combination of 175 mg/kg vitamin E and 250 mg/kg astaxanthin. The survival of shrimp by that treatment was 100.00±0.00%, specific growth rate 1.07±0.26%/day, the first level of gonad maturity growth was reached at day 14 (19.45±4.81%), the fourth level of gonad maturity was obtained at day 41, spawning rate 33.33±8.33%, fecundity 87,000±2,000 eggs, and hatching rate reached 49.00±1.53%. Keywords: astaxanthin, Litopenaeus vannamei, vitamin E ABSTRAKPeningkatan kualitas induk udang vaname Litopenaeus vannamei dapat dilakukan dengan penambahan vitamin E dan astaxanthin pada pakan. Tujuan penelitian ini adalah untuk mengevaluasi pengaruh pemberian astaxanthin dan vitamin E dengan dosis berbeda dalam pakan terhadap tingkat kematangan gonad calon induk udang vaname. Dosis yang digunakan adalah 0 mg/kg pakan astaxanthin + 0 mg/kg pakan vitamin E (kontrol/A), 500 mg/kg pakan astaxanthin (B), 350 mg/kg pakan vitamin E (C), 500 mg/kg pakan astaxanthin and 350 mg/kg pakan vitamin E (D), and 250 mg/kg pakan astaxanthin and 175 mg/kg pakan vitamin E (E). Pemberian pakan dengan penambahan vitamin E dan astaxanthin dilakukan sebanyak tiga kali, yaitu jam 06.00, 13.00, dan 20.00 WIB sebanyak 2% dari bobot udang. Hasil penelitian menunjukkan bahwa dosis optimum untuk sintasan, laju pertumbuhan spesifik, dan tingkat kematangan induk udang vaname diperoleh dengan kombinasi 175 mg/kg vitamin E dan 250 mg/ kg astaxanthin. Kelangsungan hidup udang dengan perlakuan tersebut adalah 100,00±0,00%, laju pertumbuhan spesifik 1,07±0,26%/hari, tingkat kematangan gonad pertama dicapai pada hari ke 14 (19,45±4,81%), tingkat kematangan gonad keempat diperoleh pada hari ke 41, tingkat pemijahan 33,33±8,33%, fekunditas 87.000±2.000 telur, dan tingkat penetasan mencapai 49,00±1,53%.Kata kunci: astaxanthin, udang vaname Litopenaeus vannamei, vitamin E

Effect of dietary protein, vitamin E, and vitamin C levels on the growth and gonad development of hybrid male abalone (Haliotis fulgens ♂ × H. discus hannai ♀) by the orthogonal array design

“…However, our study showed that dietary VE did not signi cantly affect the GSI of hybrid male abalone. The result was consistent with those of female shrimp (Macrobrachium nipponense) (Li et al, 2018), male Japanese quail (Coturnix coturnix Japonica) (Biswas et al, 2013), white shrimp broodstock (Litopenaeus vannamei) (Maulana et al, 2017) and female brood sh (Clarias batrachus) (Roy and Mollah, 2010). Besides, the range value showed content of VE at 0.1 g/kg was the optimal level for gonad development of abalone.…”

Effect of Dietary Carotenoids on Reproducers of Amazon River Prawn Macrobrachium amazonicum. Part 2: External Coloration, Accumulation of Astaxanthin in Body Tissues and Pigment Stability in Feed

“…In the case of males, less need for carotenoid in the formation of gametes was observed, although it is essential and can be stimulated by the consumption of astaxanthin precursors (Braga et al, 2013). In female gonads, the presence of developing eggs can influence the amount of pigment, since the deposition of pigments in crustacean eggs is essential for their perfect development (Maulana et al, 2017;Wang et al, 2018). In the case of stomachs, they can serve as a deposition site for body pigment, however previous studies have not addressed the quantification of pigments in gastric tissue.…”

Dietary DHA Oil Supplementation Promotes Ovarian Development and Astaxanthin Deposition during the Ovarian Maturation of Chinese Mitten Crab Eriocheir sinensis

“…Despite the importance of Axn [7,35] and LC-PUFAs [33,36,37] for ovarian development of E. sinensis had been demonstrated in previous studies, their combined effects on E. sinensis remain unclear. Moreover, the combined effects of dietary Axn and LC-PUFAs on crustaceans have been demonstrated in several species, including the hermaphroditic shrimp Lysmata wurdemanni [38], L. vannamei [39], and the giant tiger prawn P. monodon [40]. Considering that DHA is one of the key n-3 LC-PUFAs required for crustacean species as inspired by previous studies, this study was conducted to investigate the combined effect of dietary Axn and DHA on adult female E. sinensis regarding ovarian development, antioxidant capacity, colour parameters, carotenoid concentration, and biochemical composition during the ovarian maturation stage.…”