Supplementation Effect of Early Pregnancy Factor‐Positive Serum into Bovine <i>In Vitro</i> Fertilization Culture Medium

Ito, Kazuei; Takahashi, Masahiro; Kawahata, Kyoko; Goto, Taichi; Takahashi, Jutaro; Yasuda, Yasuhisa

doi:10.1111/j.1600-0897.1998.tb00369.x

Cited by 7 publications

(7 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using a mathematical model, Shorten et al [31] demonstrated that if IFNT signaling occurs later than day 13 of the cycle, pregnancy is not recognized irrespective of the IFNT concentration. Other candidate markers are early pregnancy factor (EPF) and EPF-like substances because EPF and EPF-like substances are detected in the serum of pregnant cows and are extraordinarily produced by embryos within 24-48 h after fertilization [32]. Ito et al [32] suggested that these EPFs regulate the maternal immune response to prevent rejection of the allograft fetus.…”

Section: Discussionmentioning

confidence: 99%

“…Other candidate markers are early pregnancy factor (EPF) and EPF-like substances because EPF and EPF-like substances are detected in the serum of pregnant cows and are extraordinarily produced by embryos within 24-48 h after fertilization [32]. Ito et al [32] suggested that these EPFs regulate the maternal immune response to prevent rejection of the allograft fetus. Additionally, the preimplantation embryo produces TGFβ and insulin-like growth factors I and II in cows [33].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Upregulation of Interferon-stimulated Genes and Interleukin-10 in Peripheral Blood Immune Cells During Early Pregnancy in Dairy Cows

et al. 2012

View full text Add to dashboard Cite

Abstract. In cows, interferon-tau (IFNT) regulates maternal recognition around days 15-19 after artificial insemination (AI). The present study hypothesized that if key target genes of IFNT are clearly upregulated in earlier stages of pregnancy, these genes could be use as indices of future pregnancy in cows. Therefore, we determined the expression of these genes in peripheral blood mononuclear leukocytes (PBMCs) and polymorphonuclear granulocytes (PMNs) during the maternal recognition period (MRP). Twenty multiparous Holstein cows were subjected to AI on day 0 and categorized into the following groups: pregnancy (Preg, n = 9), embryonic death (ED, n = 5) and non-pregnancy (NP, n = 6). Progesterone levels in the Preg group were higher than those in the NP group on days 12-21. ISG15 and OAS-1 (IFN-stimulated genes: ISGs) mRNA in PBMCs on day 8 was higher in the Preg group than in the NP group, and these mRNAs in PMNs was higher in the Preg group on day 5 than in the NP and ED groups. Interleukin-10 (IL-10, Th2 cytokine) mRNA expression increased on day 8 in the PBMCs of pregnant cows. Tumor necrosis factor α (TNFα, Th1 cytokine) mRNA expression was stable in all groups. In an in vitro cell culture experiment, IFNT stimulated mRNA expression of ISGs in both PBMCs and PMNs. IFNT stimulated IL-10 mRNA expression in PBMCs, whereas IFNT increased TNFα mRNA levels in PBMCs in vitro. The results suggest that ISGs and IL-10 could be responsive to IFNT before the MRP in peripheral blood immune cells and may be useful target genes for reliable indices of pregnancy before the MRP. Key words: Cow, Immune cells, Interferon tau, Pregnancy (J. Reprod. Dev. 58: [84][85][86][87][88][89][90] 2012) D uring the past 5 decades, milk production per cow has dramatically increased because of improved management, nutrition, and genetic selection [1,2]. In contrast, the decreases in fertility and conception rates of the modern high-producing dairy cow are the major causes of economic loss for dairy producers [2,3]. After establishment of pregnancy in domestic ruminants, the conceptus secretes interferon-tau (IFNT) as a maternal recognition factor [4]. IFNT acts in the uterus around day 16 after insemination and prevents luteolysis by inhibiting prostaglandin F 2α release, resulting in the maintenance of corpus luteum function; therefore, this period is termed the maternal recognition period (MRP) [5]. IFNT induces the synthesis and secretion of IFN-stimulated genes (ISGs) such as ISG15, 2′,5′-oligoadenylate synthetase (OAS-1), IFN regulatory factor 1, Mx1 and Mx2 not only in the uterus but also in blood cells in ewes and cows [6][7][8][9][10]. In fact, in bovine peripheral blood leukocytes, ISG15 mRNA levels were higher in pregnant cows than in nonpregnant cows on days 18 and 20 after artificial insemination (AI) [7][8][9]. Technology for the early detection of pregnancy is needed to identify nonpregnant cows and to synchronize and artificially inseminate these cows prior to the next ovulation, and many studies have focused on identify...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Upregulation of Interferon-stimulated Genes and Interleukin-10 in Peripheral Blood Immune Cells During Early Pregnancy in Dairy Cows

et al. 2012

View full text Add to dashboard Cite

show abstract

“…There have been remarkable discrepancies among molecular weights of purified EPF reported by different investigators (ranging from 10 to 21.5 kD), probably due to differences in original materials and isolation procedures. 9,24,38,39 Ito et al 13 insisted that, because Cavanagh and Morton purified EPF from human platelets, it might not be a true EPF. In order to identify true EPF, EPF has to be purified from early-pregnant serum.…”

Section: Discussionmentioning

confidence: 99%

“…EPF may be most useful for the evaluation of embryo quality in the in 6itro fertilization-embryo transfer treatment. 13 Chaperonin 10 (cpn 10) is one of the heat-shock protein (hsp) families, which act in conjunction with cpn 60 to mediate protein folding under the existence of ATP in mitochondria. 14,15 Cpn 60 is composed of two stacked 7-subunit rings with adenosine triphosphate (ATP)ase activity.…”

Section: Introductionmentioning

confidence: 99%

Chaperonin 10 in the Rat Oocytes and Early Embryos: its Expression and Activity for Early Pregnancy Factor

Kawamura

Fukuda²,

Itoh³

et al. 2000

American J Rep Immunol

Self Cite

View full text Add to dashboard Cite

show abstract

“…was EPF-like substance. 5 EPF in the pregnancy serum has been detected in many animal species, [6][7][8][9] including the Red Deer (Cer6us elaphus). 10 In almost all the animals examined, EPF was detected within 24 -48 hr after mating and it was detected until the second trimester using the rosette inhibition test.…”

Section: Introductionmentioning

confidence: 99%

Study of Early Pregnancy Factor (EPF) in Equine (Equus caballus)

Ohnuma¹,

Yokoo²,

Ito³

et al. 2000

American J Rep Immunol

Self Cite

View full text Add to dashboard Cite

show abstract

Supplementation Effect of Early Pregnancy Factor‐Positive Serum into Bovine In Vitro Fertilization Culture Medium

Abstract: The data suggest that an EPF-like substance(s) may be secreted from the in vitro fertilized bovine ovum but not from the cumulus cell, and that the EPF in the pregnant serum may accelerate the development of the bovine embryo in IVF.

Cited by 7 publications

References 27 publications

Upregulation of Interferon-stimulated Genes and Interleukin-10 in Peripheral Blood Immune Cells During Early Pregnancy in Dairy Cows

Upregulation of Interferon-stimulated Genes and Interleukin-10 in Peripheral Blood Immune Cells During Early Pregnancy in Dairy Cows

Chaperonin 10 in the Rat Oocytes and Early Embryos: its Expression and Activity for Early Pregnancy Factor

Study of Early Pregnancy Factor (EPF) in Equine (Equus caballus)

Contact Info

Product

Resources

About