Supplementary outcomes of betaine on economic and productive performance, some biochemical parameters, and lipoprotein lipase gene expression in finishing male broilers

Abstract

Egypt’s population is growing with the biggest hurdle facing the Government is to secure animal protein. Broilers provide quality protein of reasonable price. This study was conducted to investigate the outcomes of dietary organic betaine (betafin S4) on productive, epigenetic make up of lipoprotein lipase gene (LPL) promoter, some blood biochemical, and economic parameters in male broilers at finishing period. Eighty one commercial Arbor Acre Plus males, 21 days old, were randomly allocated to three groups, with three replicates each in battery cages under thermo-neutral environment till 42 days. The examined groups received yellow corn-soy basal diet, supplemented with 0 (G1), 1.5 (G2) and 3.0 g (G3) betaine/kg diet, respectively. The mRNA expression levels of LPL gene were analyzed by real-time quantitative PCR. Methylation pattern on LPL gene promoter was determined by bisulfite sequencing. Doses of betaine statistically (P ≤ .05) improved tested performance parameters; while carcass yield % and abdominal fat deposition did not achieve significant changes. The expression of LPL mRNA showed an inverse relationship with betaine dose, which illustrated as a trend toward increase in G2 and decrease in G3. Regarding serum biochemistry, both treated groups when compared to control group revealed a significant improvement (P ≤ .01) in albumin level, simultaneously, a significant increase (P ≤ .05) was recorded in uric acid and triglyceride levels, additionally, strong positive (P ≤ .01) correlation between betaine dose and previously mentioned parameters was reported. Betaine is recommended in finishing male broilers as production costs were reduced by 3.97%–4.37% per kg, respectively. In conclusion, incorporation of 0.15–0.30% organic betaine to male broilers diets during finishing period improves the growth performances.

Effects of Betaine Supplementation on Live Performance, Selected Blood Parameters, and Expression of Water Channel and Stress-Related mRNA Transcripts of Delayed Placement Broiler Chicks

“…In general, the increase in serum triglycerides in feed deprived chicks could be due to increased lipid mobilization from both the liver and yolk sac in response to energy demand. Ghasemi and Nari ( 56 ) and Yusuf et al ( 57 ) reported that the triglycerides levels in the blood to increase as a function of betaine supplementation in chickens. The mechanism underlying the triglycerides' elevation with betaine supplementation is unclear but the correlation analysis showed that there is a direct correlation with the GR expression ( r = 0.0894) and with hepatic AQP3 ( r = 0.0894).…”

Betaine Promotes Fat Accumulation and Reduces Injury in Landes Goose Hepatocytes by Regulating Multiple Lipid Metabolism Pathways

“…In the swine industry, leanness and carcass quality are improved by the addition of 0.25% betaine [ 11 ]; moreover, betaine significantly reduces the cholesterol level of chicken breast meat [ 12 ]. Yusuf et al [ 13 ] and Al-Sagan et al [ 14 ] reported that blood triglyceride (TG) levels are increased by betaine supplementation in chickens. A systematic review and meta-analysis of randomized controlled trials suggested that dietary betaine supplementation reduces human body fat [ 15 ].…”