Supplementary Methods from A New Triglycyl Peptide Linker for Antibody–Drug Conjugates (ADCs) with Improved Targeted Killing of Cancer Cells

Abstract

<p>Supplementary Methods (S1-S3). Supplementary Method S1: Synthesis of di-, tri-, tetra-glycyl, and valine-citrulline-glycine peptide linkers and catabolites. Supplementary Method S2: Test of in vitro cytotoxicity, cell-cycle inhibition, and bystander cytotoxic activity of ADCs. Supplementary Method S3: Catabolism of [3H]-labeled and non-radiolabeled CX and SMCC ADCs by cancer cells.</p>