Supplementary Methods and Fig s1-s13 from A Novel MYCN-Specific Antigene Oligonucleotide Deregulates Mitochondria and Inhibits Tumor Growth in MYCN-Amplified Neuroblastoma

Abstract

<p>Figure S1: BGA002 induces apoptosis Figure S2: BGA002 inhibits MYCN expression in a broad neuroblastoma cell-line panel Figure S3: BGA002 is a specific MYCN inhibitor Figure S4: Mitochondria structural alteration are MYCN specific inhibition. Figure S5: BGA002 leads to MYCN-specific structural alteration in mitochondria. Figure S6: BGA001 does not lead to significant MYCN-specific structural alteration in mitochondria. Figure S7: MYCN inhibition leads to mitochondrial damage Figure S8: Alterations in mitochondrial pathways identify Neuroblastoma patients with poor prognosis. Figure S9: MYCN amplified patient are enriched for the mitochondrial gene signature. Figure S10: MYCN blocking leads to MYCN specific gene expression signature inhibition in NB cells and to mitophagy reactivation. Figure S11: OPTN controls mitophagy induction after MYCN silencing Figure S12: BGA002 reverts the MYCN control of ROS generation by TRAP1 down-regulation in MNA-NB cells. Figure S13: BGA002 causes elimination of MYCN-NB in mice</p>