Supplementary Materials and Methods, Figures S1 - S8 from Periprostatic Adipose Tissue Favors Prostate Cancer Cell Invasion in an Obesity-Dependent Manner: Role of Oxidative Stress

Abstract

<p>Supplementary Figure S1. Coculture with preadipocytes has no effect on cancer cell invasion and coculture with mature adipocytes has no effect on prostate cancer cell number. Supplementary Figure S2. Adrenergic receptors do not control adipocyte lipolysis in response to tumor cell secretions. Supplementary Figure S3. The metabolic remodeling towards uncoupled FAO observed in cocultivated cells is not involved in the increase in prostate cancer invasion induced by adipocytes. Supplementary Figure S4. ROS positively regulate prostate cancer cells invasion. Supplementary Figure S5. 10mM NAC and 10μM DPI do not affect PC3 viability. Supplementary Figure S6. Coculture and palmitate stimulate tumor invasion through NOX in Du-145 cells. Supplementary Figure S7. NOX5 invalidation leads to a strong decrease in cell survival.</p>