Supplementary figures S1-S18 from Loss of TRIM29 Alters Keratin Distribution to Promote Cell Invasion in Squamous Cell Carcinoma

Abstract

<p>Supplemental figures Supplemental Fig. 1. TRIM29 is highly expressed in stratified epithelial tissues. Supplemental Fig. 2. The expression levels of TRIM29 protein are as high in benign skin tumors as they are in normal epidermis. Supplemental Fig. 3. The expression level of TRIM29 is low in keratoacanthoma-like SCC, but not in keratoacanthoma. Supplemental Fig. 4. Reduced TRIM29 RNA expression is associated with DNA methylation in cutaneous SCC. Supplemental Fig. 5. TRIM29 expression is silenced in cutaneous SCC cells. Supplemental Fig. 6. The cell proliferation of TRIM29-knockdown cancer cells does not differ significantly from that of the control cells. Supplemental Fig. 7. siRNA-mediated TRIM29-knockdown does not affect cell proliferation. Supplemental Fig. 8. TRIM29 regulates migration of SCC cells and immortalized epidermal keratinocytes. Supplemental Fig. 9. TRIM29 regulates migration of SCC cells and immortalized epidermal keratinocytes. Supplemental Fig. 10. TRIM29 knockdown increases cell invasion in SCC cells and immortalized epidermal keratinocytes. Supplemental Fig. 11. TRIM29 knockdown increases cell invasion in oral SCC cells. Supplemental Fig. 12. TRIM29 knockdown enhances the cancer cell metastasis. Supplemental Fig. 13. The top 20 list of proteins bound with FLAG-TRIM29 in mass spectrometry analysis. Supplemental Fig. 14. TRIM29 co-localizes with FAM83H. Supplemental Fig. 15. TRIM29 co-localizes with FAM83H. Supplemental Fig. 16. The zinc finger, B-box, coiled-coil, and C-terminal domains of TRIM29 are necessary for the formation of the TRIM29-keratin-FAM83H complex. Supplemental Fig. 17. Schema of the keratin distribution patterns. Supplemental Fig. 18. Knockdown of TRIM29 alters the distribution of keratins.</p>