Supplementary Figures from TNF Receptor-2 Facilitates an Immunosuppressive Microenvironment in the Liver to Promote the Colonization and Growth of Hepatic Metastases

Abstract

<p>Supplementary Fig 1. Identification of hepatic cells that express TNFR2. Supplementary Fig 2. Loss of TNFR2 expression does not affect the induction of CAM expression on LSEC. Supplementary Fig 3. Additional characterization of MDSC isolated from wild type and TNFR2-/- mice. Supplementary Fig 4. The recruitment of Treg cells to the liver is not reduced in TNFR1-/- mice. Supplementary Fig 5. TNFR2 expression is not required for tumor or TNF-α - induced upregulation of hepatic CXCR2 chemokines. Supplementary Fig 6. Loss of TNFR2 does not affect the expression of CXCR1 and CXCR2 on MDSC. Supplementary Fig 7. Reduced TNFR2 expression in the livers of mice treated with TNFR2 ASO. Supplementary Fig 8. HLA-DR is widely expressed in the normal liver.</p>