Supplementary Figures and Tables from Early Loss of Histone H2B Monoubiquitylation Alters Chromatin Accessibility and Activates Key Immune Pathways That Facilitate Progression of Ovarian Cancer

Abstract

<p>Supplementary Figures and Tables - Figure S1. RNF40 somatic copy number alterations in a cohort of 579 HGSOC cases from The Cancer Genome Atlas (TCGA). Heterozygous loss of RNF40 is present in 36% of HGSOCs. Figure S2A. H2Bub1 stain intensity dot plot. Figure S2B. Distribution of H2Bub1 immunoreactivity (% positive cells). Figure S2C. Immunohistochemical analysis of Total H2B and H2Bub1 levels in invasive high-grade serious ovarian cancer. Figure S3. Stable RNF20 and H2Bub1 depletion escalates oncogenic behavior in FTSEC cell lines. Figure S4. Transient RNF20 and H2Bub1 depletion escalates oncogenic behavior in FTSECs. Figure S5. RNF20-depletion changes chromatin accessibility and redistributes open chromatin. Figure S6. RNF20-depletion changes chromatin accessibility and alters gene expression of immune signaling pathways in FTSEC cells. Figure S7. RNF20 and H2Bub1 deficiency alters the levels of cytokines. Figure S8. Representative images for RNA in situ hybridization of IL6 expression in FFPE tumor and normal tissue sections. Supplementary Table 1. Antibody information and staining conditions for immunohistochemistry (IHC).</p>