Supplementary figures and figure legends from Targeting a Single Alternative Polyadenylation Site Coordinately Blocks Expression of Androgen Receptor mRNA Splice Variants in Prostate Cancer

Abstract

<p>This file contains supplementary figures S1-S10 and corresponding figure legends. Figure S1: TALEN activity and genetic correction of 22Rv1 sub-lines. Figure S2: 5' and 3' breakpoint sequence junctions of 22Rv1 undup1-3 sub-lines. Figure S3: AR subcellular localization in 22Rv1-undup3 cell line. Figure S4: AR target gene expression panel in 22Rv1 and gene corrected sublines. Figure S5: Knockdown of splicing factors U2AF2, SRSF1, and PTBP1. Figure S6: Blockade of the AR CE3 PAS blocks AR-V expression in LNCaP and VCaP cells. Figure S7: Knockdown of CE3 in 22Rv1 and VCaP cells. Figure S8: Mutual exclusivity matrix depicting co-occurrence of alterations in the core CPSF complex. Figure S9: Confirmation of CPSF complex gene knockdown. Figure S10: Knockdown of CPSF1 in LNCaP95 cells.</p>