Supplementary Figures 1-7 from Mevalonate Pathway Provides Ubiquinone to Maintain Pyrimidine Synthesis and Survival in p53-Deficient Cancer Cells Exposed to Metabolic Stress

Abstract

<p>(1) Figure S1 shows Metabolic flux analysis of p53+/+ and p53-/- cells in monolayer and spheroid culture. (2) Figure S2 shows Mechanism of induction of SREBP2 target genes in p53-deficient spheroid cultures. (3) Figure S3 shows Induction of cell cycle arrest and apoptosis by simvastatin in spheroid cultures. (4) Figure S4 shows Blockade of cholesterol and ubiquinone synthesis by statins. (5) Figure S5 shows Effect of simvastatin on TCA cycle flux and failure of ubiquinone to restore viability in monolayer cells. (6) Figure S6 shows Inhibition of apoptosis in p53-deficient spheroid cultures by uridine. (7) Figure S7 shows Genotyping of cells used for organoid cultures and lack of effect of leflunomide on intestinal hyperproliferation.</p>