Supplementary Figures 1 - 6, Table 1, Methods from Pro-Oncogenic Role of Alternative p38 Mitogen-Activated Protein Kinases p38γ and p38δ, Linking Inflammation and Cancer in Colitis-Associated Colon Cancer

Abstract

<p>Figure S1. Effect of p38gamma and p38delta deletion in early stages of colitis-associated colon cancer induced by AOM/DSS treatment. Figure S2. p38gamma and p38deltaexpression in AOM/DSS-treated and untreated mouse colon. WT, p38gamma /-, p38delta/- or p38gamma/delta/- mice were AOM/DSS-treated and untreated. At 9 weeks post-AOM injection, colon extracts were prepared for mRNA and protein analysis. Figure S3. p38gamma or p38deltadeletion has no significant effect on body weight and colon length in the course of DSS-induced colitis. WT and p38gamma/delta/- mice were treated with 3% DSS in drinking water for 5 days. Figure S4. p38gamma/delta-/- mice show increased IEC apoptosis after DSS treatment. Figure S5. ERK1/2 phosphorylation, but not IkappaBalpha proteolysis, was reduced in p38gamma/delta-/- colon cells compared to WT. Figure S6. Effect of p38gamma/deltadeletion in the expression of chemokine receptors and in the recruitment of neutrophils, macrophages and Treg cells. Table SI. Primer sequences used for gene expression.</p>