This experiment was aimed to observe rumen fermentation kinetics of alternative supplements, i.e. Moringa oleifera and Peltiphyllum peltatum leaves added to maize silage diet as compared to a conventional supplement (barley-soya). A total of six treatments were investigated in the present study, which consisted of: maize silage (A), M. oleifera (B), P. peltatum (C), maize silage + concentrate (56:44, w/w; D), maize silage + M. oleifera (56:44, w/w; E), and maize silage + concentrate + P. peltatum (47:37:16, w/w/w; F). The feeds were incubated in vitro in three replicates (completely randomized) using the Reading Pressure Technique system. Approximately 800 mg of the feeds were mixed by 75 ml of buffered rumen liquor. The incubation was carried out up to 12 h in bottles and gas pressure was recorded and released in regular intervals. Repeated sampling was conducted for analysis of fermentation products at 1, 6 and 12 h of incubation period. The results showed that the nutritional quality of Moringa and its fermentation pattern was comparable to that of barley-soya concentrate. Plain Moringa incubation resulted the highest production of iso-SCFA and soluble protein concentration among all treatments after 12 h incubation (P<0.05). Incubation of sole Peltiphyllum peltatum highly hampered the fermentation rate. Production of iso-SCFA both for plain Peltiphyllum and its mixture were comparatively low. Supplementation of Peltiphyllum increased significantly soluble protein concentration during 12 h incubation (P<0.05). Peltiphyllum also had a very low C 2 +C 4 /C 3 ratio compared to other treatments. It could be concluded that Moringa oleifera is a potential alternative supplement to replace either partially or completely concentrate as a conventional supplement, and Peltiphyllum peltatum supplementation could reduce excessive protein degradation and fermentation of the concentrate in the rumen.