Supplementary Experimental Procedures from Hepatic Endothelial Notch Activation Protects against Liver Metastasis by Regulating Endothelial-Tumor Cell Adhesion Independent of Angiocrine Signaling

Abstract

<p>Additional information about the following materials and methods are enclosed here: Animal experiments, bioluminescence imaging, blood parameters, embryo and organ preparation, routine and immuno histology, microarray analysis, RNA in situ hybridization, image acquisition and processing, confocal microscopy, LSEC isolation, details of antibodies and qRT-PCR analysis.</p>