Supplementary Data from Replication Gaps Underlie BRCA Deficiency and Therapy Response

Abstract

<p>Supplemental Figures 1-6. Figure S1: BRCA-deficient cancer cells fail to restrain replication in the presence of stress and ssDNA gaps develop. Figure S2: Replication restraint, depletion, and PDX controls. Figure S3: Depletion of SMARCAL1 or inhibition of MRE11 restores FP, but do not predict patient response and do not suppress ssDNA gaps, which are distinct from fork degradation. Figure S4: Fanconi Anemia Patient Fibroblasts with a RAD51 T131P Mutant Allele (HR Proficient, FP Deficient) Generate ssDNA Gaps, and FP is restored by RADX depletion. Figure S5: Apoptosis and Z-VAD-FMK Controls. Figure S6: Prediction Table of BRCAness and Chemoresponse.</p>