Supplementary Data from Matricellular Protein WISP2 Is an Endogenous Inhibitor of Collagen Linearization and Cancer Metastasis

Abstract

<p>Supplementary Materials and Methods; Supplementary Figures S1 to S8; Supplementary Figure S1. WISP2 inhibits WISP1-induced Col I linearization; Supplementary Figure S2. WISP2 is less abundant in tumors than in adjacent normal tissues and is an inhibitor of WISP1-induced tumor cell invasion though Col I; Supplementary Figure S3. The C-terminal domain of WISP1 drives WISP1-induced cell invasion through Col I but is dispensable for WISP1-Col I binding; Supplementary Figure S4. Wisp1 is expressed by tumor cells and cancer-associated fibroblast (CAF)-enriched stromal cells isolated from primary breast tumors; Supplementary Figure S5. WISP2 and WISP1DCT block TGFb1-induced cell invasion through Col I by acting as WISP1 antagonists; Supplementary Figure S6. WISP2 limits collagen linearization but does not affect total type I collagen levels in 4T1 breast tumors; Supplementary Figure S7. WISP2 limits collagen linearization in aggressive 4T1-Wisp1 tumors and inhibits breast cancer metastasis; Supplementary Figure S8. WISP1 promotes whereas WISP2 inhibits human breast cancer metastasis; Supplementary Tables S1 to S3; Supplementary Table S1. gBlock sequence for the generation of pCDH-EF1-Wisp2+CTT2A- puro from pCDH-EF1-Wisp2-T2A-puro; Supplementary Table S2. Primers used for cloning; Supplementary Table S3. gRNAs targeting Wisp1 and of non-targeting control gRNAs; Supplementary References</p>