Supplemental Leuconostoc mesenteroides strain NTM048 attenuates imiquimod‐induced psoriasis in mice

Abstract

Aims Psoriasis, a chronic inflammatory skin disease, is associated with altered intestinal microbiota. Here, we investigated the ameliorative effect of Leuconostoc mesenteroides NTM048 strain in imiquimod (IMQ)‐induced psoriasis in mice. Methods and Results Mice were administered NTM048 for 21 days alongside the topical application of IMQ on the dorsal skin for 6 consecutive days. IMQ induced psoriatic symptoms such as erythema and scaling and also upregulated interleukin (IL)‐17, a key effector cytokine of psoriasis, in the skin. Supplemental NTM048 suppressed these abnormalities, increased the levels of plasma deoxycholic acid (DCA), a secondary bile acid and altered the faecal microbiota composition, as indicated by the increased abundance of Akkermansia and decreased abundance of Staphylococcus and Streptococcus. Notably, DCA treatment of murine splenocytes reduced IL‐17 production. Conclusions The NTM048‐mediated reduction of psoriasis was shown to involve the downregulation of IL‐17 in mouse skin, which was possibly associated with the plasma DCA derived from intestinal microbiota. Significance and Impact of the Study Our findings propose not only a novel approach for psoriasis reduction but also a crosstalk between the skin and intestine in psoriasis.

Microbiome Modulation as a Therapeutic Approach in Chronic Skin Diseases

“…Ogawa et al showed that administering Leuconostoc mesenteroides NTM048 to imiquimod-induced mice suppressed erythema, scaling, upregulated IL-17 production, increased the levels of plasma deoxycholic acid and altered the faecal microbiota composition. Changes in the gut microbiome were indicated by the increased abundance of Akkermansia and a decreased abundance of Staphylococcus and Streptococcus [100]. The only study concerning a topical application of probiotics was conducted by Rather et al Application of ethanol extract (SEL001) isolated from L. sakei proBio-65 resulted in an inhibition of the imiquimod-induced changes in the skin, as well as decreased IL-19, IL-17A and IL-23 levels [98].…”

Ready-To-Eat Rocket Salads as Potential Reservoir of Bacteria for the Human Microbiome

“…The phyllosphere of numerous vegetables is a well-known source of LAB ( 27 , 28 ), which have been proposed as promising cultures to be exploited for food preservation and probiotic applications ( 29 – 32 ). In particular, more than 20% of all LAB isolates in our study belonged to Leuconostoc mesenteroides , a species that has been frequently shown to exert probiotic activities in vitro and in vivo ( 29 , 33 – 36 ) and that includes several strains already employed in commercially available probiotic products. In addition, about 14% of LAB isolates were ascribed to Weissella species, a genus constituting bacteria frequently isolated from food that are emerging for their promising health-promoting activities ( 37 ), such as the demonstrated properties in the oral cavity for the treatment of halitosis ( 38 ).…”

Propionate and Dietary Fermentable Fibers Upregulate Intestinal Heat Shock protein70 in Intestinal Caco-2 Cells and Mouse Colon

“…SCFAs in the cecal contents were determined by ultraperformance liquid chromatography−mass spectrometry as described previously. 34 Data Calculation and Statistical Analysis. All data are presented as means ± standard error of the means (SEM).…”

“…Colonic epithelial cells were isolated as described previously and subjected to immunoblot analysis as described above. SCFAs in the cecal contents were determined by ultraperformance liquid chromatography–mass spectrometry as described previously …”