Supplemental Figures from Glycogen Synthase Kinase-3β: A Prognostic Marker and a Potential Therapeutic Target in Human Bladder Cancer

Abstract

<p>Supplemental Figure 1. Immunohistochemical staining of GSK-3β in low (A-C) and high (D-F) grade urothelial tumors. Supplemental Figure 2. Hoechst staining of T24 cancer cells treated with 3 distinct small molecule inhibitors of GSK-3 (AR-A014418, SB-415286, and TDZD-8). The arrows point at apoptotic cells. The percentage of apoptotic cells is shown as indicated on each picture. Supplemental Figure 3. HT1376 and T24 cancer cells were treated with indicated doses of AR-A014418; 48, 72 and 96 h after treatment, cell lysates were separated by SDS-PAGE, transferred to PVDF membrane and probed with antibodies to indicated proteins.</p>