Superporous collagen‐sericin scaffolds

Lungu, Adriana; Kaya, Mădălina Georgiana Albu; Stancu, Izabela‐Cristina; Florea, Nicoleta Mihaela; Vasile, Eugeniu; Iovu, Horia

doi:10.1002/app.37934

Cited by 41 publications

(36 citation statements)

References 40 publications

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“…Type I fibrillar COLL was extracted from calf hide in gel form with an initial concentration of 1.54% (w/w) by acid and alkaline treatments as previously described [35]. Silk sericin (SS) (purchased from Sigma-Aldrich -Japan) in different weight ratios was added to COLL gel, keeping the COLL concentration constant (1.2%).…”

Section: Collagen-sericin Hydrogel Preparationmentioning

confidence: 99%

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Dose-related effects of sericin on preadipocyte behavior within collagen/sericin hybrid scaffolds

Mitran

Kaya

Vasile

et al. 2015

Progress in Natural Science: Materials International

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This paper aims at demonstrating the biocompatibility of recently developed 3D hydrogel scaffolds containing the same amount of collagen (COLL) and variable concentrations of sericin (SS) in order to find the most suitable formula for adipose tissue engineering (ATE) applications. These scaffolds were obtained by COLL crosslinking with glutaraldehyde followed by freeze-drying and, subsequently, seeded with 3T3-L1 preadipocytes. Scanning electron microscopy studies revealed the scaffolds' architecture and cellular colonization. Also, in vitro biocompatibility of the developed scaffolds was evaluated by LDH and MTT assays and Live/Dead analysis of 3T3-L1 preadipocyte populating these 3D matrices. The best results in terms of cell survival and proliferation status were obtained in the case of the hybrid COLL scaffold containing 40% SS (COLL-SS4). Furthermore, the biological performance of the analyzed COLL-based hydrogels at 5-and 8-days post-seeding was found to decrease as follows: COLL-SS4 4 COLL-SS24 COLL 4 COLL-SS6. Consequently, our study highlights that hybrid scaffolds obtained by the addition of variable concentrations of SS to a constant COLL composition positively influences the behavior of 3T3-L1 cells with the exception of the COLL-SS6 matrix (60% SS). Altogether, the data obtained recommend SS as a component of COLL-based hydrogels providing them with features that may be useful in ATE applications.

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Section: Collagen-sericin Hydrogel Preparationmentioning

confidence: 99%

“…The high water content of hydrogels combined with a controllable network structure provides a natural synergy with the human body. Therefore, the preparation of some COLL-SS hydrogel scaffolds, having different grade of porosity and interconnected pores [35], has become one of the major challenges in ATE.…”

Section: Introductionmentioning

confidence: 99%

Dose-related effects of sericin on preadipocyte behavior within collagen/sericin hybrid scaffolds

Mitran

Kaya

Vasile

et al. 2015

Progress in Natural Science: Materials International

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“…It is widely used in the pharmaceutical and medical fields [23]. Although collagen has many advantages [24], the reconstructed collagen-based scaffolds usually suffer from poor physicochemical properties which limit their application [25].…”

Section: Introductionmentioning

confidence: 99%

Hydrophilic PCU scaffolds prepared by grafting PEGMA and immobilizing gelatin to enhance cell adhesion and proliferation

Shi

Yuan

Khan

et al. 2015

Materials Science and Engineering: C

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“…This is clearly due to the existence of the GAGs in the collagen, because the GAGs are well-known for its high water absorptivity and sustainability. [18] On the other hand, the water uptake was similar to that to the collagen matrix in the case of collagen-heparin complex. It is not clear why the heparin was different from other GAGs complexes, despite the fact that the amount of collagen was highest of all.…”

Section: Physical Characteristics Of Collagen-gags Complex Matrixmentioning

confidence: 75%

Preparation Fibrillized Collagen‐Glycosaminoglycan Complex Matrix Using Fibrillogenesis

Nam

Kimura

Kishida

2015

Macromolecular Symposia

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Summary: In order to replicate the function of the native dermis, we prepared fibrillized collagen-GAGs complex matrix possessing the similar structure as native dermis structure using collagen re-arrangement phenomenon in the physiological conditions. For GAGs, hyaluronic acid, chondroitin-6-sulfate, and heparin were used. In the process of preparing a collagen-GAGs complex matrix, the fibrillization of the collagen was observed when hyaluronic acid and chondroitin-6-sulfate were mixed together with collagen in the physiological conditions. However, no fibrillization was observed when collagen was mixed with heparin to prepare a collagen-heparin complex matrix. The swelling ratio and the mechanical strength had changed by the fibrillization, where low water absorption and brittleness was shown for collagenheparin complex matrix which is not fibrillized. This study shows that hyaluronic acid and chondroitin-6-sulfate contributes to the fibrillization of the collagen while the heparin forms intercalation with collagen molecules preventing fibrillogenesis.

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Superporous collagen‐sericin scaffolds

Cited by 41 publications

References 40 publications

Dose-related effects of sericin on preadipocyte behavior within collagen/sericin hybrid scaffolds

Dose-related effects of sericin on preadipocyte behavior within collagen/sericin hybrid scaffolds

Hydrophilic PCU scaffolds prepared by grafting PEGMA and immobilizing gelatin to enhance cell adhesion and proliferation

Preparation Fibrillized Collagen‐Glycosaminoglycan Complex Matrix Using Fibrillogenesis

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