Superparamagnetic Nanoparticles as a Powerful Systems Biology Characterization Tool in the Physiological Context

Salaklang, Jatuporn; Steitz, Benedikt; Finka, Andrija; O’Neil, Conlin P.; Moniatte, Marc; Vlies, André J. van der; Giorgio, Todd D.; Hofmann, Heinrich; Hubbell, Jeffrey A.; Petri‐Fink, Alke

doi:10.1002/ange.200800357

Cited by 6 publications

(6 citation statements)

References 12 publications

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“…Interestingly, another study has shown that the improvement of in vitro cellular uptake of larger carboxy-dextran-coated SPIOs is rather due to surface properties than particle size, which may have large impacts on monocyte phagocytosis [31]. Studies have also shown that cationic surfaces facilitate cellular internalization [43, 44]. Accordingly, polycationic protamine sulfate was reported to bind on the dextran coating of iron oxide particles and modify the distribution of positive and negative surface charges, making these particles easily adhere to the cell membrane and facilitating uptake by the cell [45].…”

Section: Discussionmentioning

confidence: 99%

A New Nano-sized Iron Oxide Particle with High Sensitivity for Cellular Magnetic Resonance Imaging

Chen

Zhang

et al. 2010

Mol Imaging Biol

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Purpose-In this study, we investigated the labeling efficiency and magnetic resonance imaging (MRI) signal sensitivity of a newly synthesized, nano-sized iron oxide particle (IOP) coated with polyethylene glycol (PEG), designed by Industrial Technology Research Institute (ITRI).Procedures-Macrophages, bone-marrow-derived dendritic cells, and mesenchymal stem cells (MSCs) were isolated from rats and labeled by incubating with ITRI-IOP, along with three other iron oxide particles in different sizes and coatings as reference. These labeled cells were characterized with transmission electron microscopy (TEM), light and fluorescence microscopy, phantom MRI, and finally in vivo MRI and ex vivo magnetic resonance microscopy (MRM) of transplanted hearts in rats infused with labeled macrophages.Results-The longitudinal (r 1 ) and transverse (r 2 ) relaxivities of ITRI-IOP are 22.71 and 319.2 s −1 mM −1 , respectively. TEM and microscopic images indicate the uptake of multiple ITRI-IOP particles per cell for all cell types. ITRI-IOP provides sensitivity comparable or higher than the other three particles shown in phantom MRI. In vivo MRI and ex vivo MRM detect punctate spots of hypointensity in rejecting hearts, most likely caused by the accumulation of macrophages labeled by ITRI-IOP.Conclusion-ITRI-IOP, the nano-sized iron oxide particle, shows high efficiency in cell labeling, including both phagocytic and non-phagocytic cells. Furthermore, it provides excellent sensitivity in T 2 *-weighted MRI, and thus can serve as a promising contrast agent for in vivo cellular MRI.

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Section: Discussionmentioning

confidence: 99%

A New Nano-sized Iron Oxide Particle with High Sensitivity for Cellular Magnetic Resonance Imaging

Chen

Zhang

et al. 2010

Mol Imaging Biol

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“…A novel way of synthesizing and functionalizing ultrasmall Fe 3 O 4 NPs as contrast agents for in vivo tumor detection using MRI was presented by Chen and Sun [152], which was based on Mannich ligation between the iron chelated cathecol unit (157) and the -amino group of the lysine within the c(RGDyK) integrin binder. The schematic illustration of this chemistry is shown in Scheme 9.…”

Section: Optical and Magnetic Resonance Imagingmentioning

confidence: 99%

“…A superparamagnetic iron oxide nanoparticle-based (SPION) probe for dual-modality magnetic biology characterization/imaging with dual-targeting capability, was recently introduced by Petri-Fink et al [157] by exploiting the polyvalent nature of a SPION corona. To this purpose, the APS-coated SPIONs (APS = aminopropyltriethoxysilane) were manufactured by appending a fluorescent 7-hydroxycoumarin label, a mitochondrial targeting 22-mer peptide (MTP), and a v 3 -targeting c(RGDfK) moiety.…”

Section: Multimodal Display Systemsmentioning

confidence: 99%

Targeting αvβ3 Integrin: Design and Applications of Mono- and Multifunctional RGD-Based Peptides and Semipeptides

Auzzas

Zanardi²,

Battistini³

et al. 2010

CMC

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The outstanding physio-pathological role played by integrin receptors in living subjects motivates the enormous interest shown by scientists worldwide for this topic. More than twenty years of research has spanned across the structural and functional elucidation of these proteins and over their antagonism-based biomedical applications. The proof-of concept stage, aimed at identifying potent inhibitors, covered a decade of studies, and paved the way for a more advanced era of research where these antagonist molecules were thrown into the deep end of applicative studies. This review intends to summarize the major efforts conducted thus far and focuses on the design, synthesis and biomedical applications of cyclic RGD-containing alpha(v)beta(3) integrin antagonists, in both their small and macromolecular formats. In particular, Chapters 1 and 2 offer a comprehensive outlook on the rational basis for the design of integrin inhibitors, Chapter 3 chronicles the biological and medical applications of monofunctional RGD integrin ligands both in their monomeric and multimeric asset, and Chapter 4 illustrates the potential of RGD-based multifunctional systems in molecular medicine.

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“…A magnetic separation technique and the magnetic properties of SPIONs are useful to overcome these problems. Moreover, this technique creates the precise gradient removal of the NP-attached protein coronas3536. Thus, this study aims to probe the corona compositions of two important types of polymers (dextran and polyvinyl alcohol; PVA) with various surface charges using a magnetic separation method.…”

mentioning

confidence: 99%

Protein Corona Composition of Superparamagnetic Iron Oxide Nanoparticles with Various Physico-Chemical Properties and Coatings

Sakulkhu

Mahmoudi

Maurizi

et al. 2014

Sci Rep

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218

179

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Because of their biocompatibility and unique magnetic properties, superparamagnetic iron oxide nanoparticles NPs (SPIONs) are recognized as some of the most prominent agents for theranostic applications. Thus, understanding the interaction of SPIONs with biological systems is important for their safe design and efficient applications. In this study, SPIONs were coated with 2 different polymers: polyvinyl alcohol polymer (PVA) and dextran. The obtained NPs with different surface charges (positive, neutral, and negative) were used as a model study of the effect of surface charges and surface polymer materials on protein adsorption using a magnetic separator. We found that the PVA-coated SPIONs with negative and neutral surface charge adsorbed more serum proteins than the dextran-coated SPIONs, which resulted in higher blood circulation time for PVA-coated NPs than the dextran-coated ones. Highly abundant proteins such as serum albumin, serotransferrin, prothrombin, alpha-fetoprotein, and kininogen-1 were commonly found on both PVA- and dextran-coated SPIONs. By increasing the ionic strength, soft- and hard-corona proteins were observed on 3 types of PVA-SPIONs. However, the tightly bound proteins were observed only on negatively charged PVA-coated SPIONs after the strong protein elution.

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Superparamagnetic Nanoparticles as a Powerful Systems Biology Characterization Tool in the Physiological Context

Cited by 6 publications

References 12 publications

A New Nano-sized Iron Oxide Particle with High Sensitivity for Cellular Magnetic Resonance Imaging

A New Nano-sized Iron Oxide Particle with High Sensitivity for Cellular Magnetic Resonance Imaging

Targeting αvβ3 Integrin: Design and Applications of Mono- and Multifunctional RGD-Based Peptides and Semipeptides

Protein Corona Composition of Superparamagnetic Iron Oxide Nanoparticles with Various Physico-Chemical Properties and Coatings

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Superparamagnetic Nanoparticles as a Powerful Systems Biology Characterization Tool in the Physiological Context

Cited by 6 publications

References 12 publications

A New Nano-sized Iron Oxide Particle with High Sensitivity for Cellular Magnetic Resonance Imaging

A New Nano-sized Iron Oxide Particle with High Sensitivity for Cellular Magnetic Resonance Imaging

Targeting &#945;v&#946;3 Integrin: Design and Applications of Mono- and Multifunctional RGD-Based Peptides and Semipeptides

Protein Corona Composition of Superparamagnetic Iron Oxide Nanoparticles with Various Physico-Chemical Properties and Coatings

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Targeting αvβ3 Integrin: Design and Applications of Mono- and Multifunctional RGD-Based Peptides and Semipeptides