Superparamagnetic maghemite nanoparticles from solid-state synthesis – Their functionalization towards peroral MRI contrast agent and magnetic carrier for trypsin immobilization

Kluchova, Katerina; Zbořil, Radek; Tuček, Jiří; Pecova, Michaela; Zajoncová, Ludmila; Šafařı́k, Ivo; Mashlan, M.; Markova, Ingrid; Jančík, Dalibor; Šebela, Marek; Bartonkova, H.; Bellesi, Vassiliki; Novák, Pavel; Petridis, Dimitris

doi:10.1016/j.biomaterials.2009.02.023

Cited by 156 publications

(89 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Particles such as crosslinked iron oxide [69,70], ultrasmall superparamagnetic iron oxide [71,72], and monocrystalline iron oxide nanoparticles [73,74] had all been developed as imaging agents in magnetic resonance imaging. Magnetic particles have been used to immobilize enzymes in order to enhance bioelement stability, ease separation from the reaction mixture, and increase catalyst stability, and they have been proposed for biotechnological applications [75] or for analytical devices, such as biosensors [76,77]. Furthermore, nanomedicine is an emerging field that uses nanoparticles to facilitate the diagnosis and treatment of diseases.…”

Section: Magnetic Nanoparticlesmentioning

confidence: 99%

Enzyme immobilization: an update

et al. 2013

View full text Add to dashboard Cite

Compared to free enzymes in solution, immobilized enzymes are more robust and more resistant to environmental changes. More importantly, the heterogeneity of the immobilized enzyme systems allows an easy recovery of both enzymes and products, multiple re-use of enzymes, continuous operation of enzymatic processes, rapid termination of reactions, and greater variety of bioreactor designs. This paper is a review of the recent literatures on enzyme immobilization by various techniques, the need for immobilization and different applications in industry, covering the last two decades. The most recent papers, patents, and reviews on immobilization strategies and application are reviewed.

show abstract

Section: Magnetic Nanoparticlesmentioning

confidence: 99%

Enzyme immobilization: an update

et al. 2013

View full text Add to dashboard Cite

show abstract

“…This phenomenon is usually observed in nanoparticle interacting systems. Such a reduction of maximum magnetization can be ascribed to surface effects arising from broken symmetry and reduced coordination of atoms lying at the surface of maghemite nanoparticles and also to a high degree of interparticle interactions [15]. Electrostatic stabilization is a simple way for imparting high stability to maghemite suspensions, which are sensitive to the changes in the pH.…”

Section: Figure 1 Tem Images Of Maghemite Nanoparticles For Stabilitmentioning

confidence: 99%

The Effect of pH and Time on The Stability of Superparamagnetic Maghemite Nanoparticle Suspensions

Nurdin¹,

Ridwan²,

Satriananda³

2016

MATEC Web of Conferences

View full text Add to dashboard Cite

Abstract. Maghemite (γ-Fe 2 O 3 ) nanoparticles have been synthesized using a chemical co-precipitation method. The morphology and particle size is characterized using Transmission Electron Microscopy (TEM), and magnetic characterization using Alternating Gradient Magnetometry (AGM). The stability of the maghemite nanoparticles suspension were studied at different pH and time of storage. Dynamic Light Scattering (DLS) and Zeta Potential were conducted to determine the stability of the suspensions. TEM observation showed that the particles size is 9.6 nm and have spherical morphology. The particles showed superparamagnetic behavior with saturation magnetization 25.5 emu/g. The suspensions are stable in the acidic condition at pH 4 and alkaline condition at pH 10. The suspensions remain stable after 4 weeks of storage.

show abstract

“…Generally, MRCP is performed without any special preparation, but the presence of ascites prompted radiologists to suppress the signal intensity of the bowel lumen. As recently described, one possibility is filling the bowel lumen with superparamagnetic iron oxide (SPIO) based negative per-oral contrast agent [3][4][5] , which allows better differentiation of the extrahepatic biliary tree. The need for bowel opacification with the use of oral contrast medium is mandatory to differentiate between collapsed or fluid-filled bowel loops, extrahepatic biliary ducts, intra-abdominal organs, ascites and pathological lesions.…”

Section: Introductionmentioning

confidence: 99%

Magnetic resonance cholangiopancreatography (MRCP) using new negative per-oral contrast agent based on superparamagnetic iron oxide nanoparticles for extrahepatic biliary duct visualization in liver cirrhosis

Poláková¹,

Mocikova²,

Purova³

et al. 2016

BIOMED PAP

Self Cite

View full text Add to dashboard Cite

Background and Aims. Magnetic resonance cholangiopancreatography (MRCP) is often used for imaging of the biliary tree and is required by surgeons before liver transplantation. Advanced liver cirrhosis and ascites in patients however present diagnostic problems for MRCP. The aim of this study was to find out if the use of our negative per-oral contrast agent containing superparamagnetic iron oxide nanoparticles (SPIO) in MRCP is helpful for imaging of hepatobiliary tree in patients with liver cirrhosis. Methods. Forty patients with liver cirrhosis were examined on a 1.5 T MR unit using standard MRCP protocol. Twenty patients (group A) underwent MRCP after administration of per-oral SPIO contrast agent 30 min before examination. In group B, twenty patients were examined without per-oral bowel preparation. Ascites was present in eleven patients from group A and in thirteen patients in group B. Four radiologists analyzed MR images for visibility and delineation of the biliary tree. χ 2 tests were used for comparison of the visibility of intrahepatic and extrahepatic biliary ducts in patients with and without ascites. Results. Better extrahepatic biliary duct visualization and visibility of extraluminal pathologies in patients with ascites was proved after administration of SPIO contrast agent. No statistically significant difference between group A and B was found for visualization of extrahepatic biliary ducts in patients without ascites. Delineation of intrahepatic biliary ducts was independent on bowel preparation. Conclusions. Application of our negative per-oral SPIO contrast agent before MRCP improves the visualization of extrahepatic biliary ducts in patients with ascites which is helpful during the liver surgery, mainly in liver transplantation.

show abstract

Superparamagnetic maghemite nanoparticles from solid-state synthesis – Their functionalization towards peroral MRI contrast agent and magnetic carrier for trypsin immobilization

Cited by 156 publications

References 62 publications

Enzyme immobilization: an update

Enzyme immobilization: an update

The Effect of pH and Time on The Stability of Superparamagnetic Maghemite Nanoparticle Suspensions

Magnetic resonance cholangiopancreatography (MRCP) using new negative per-oral contrast agent based on superparamagnetic iron oxide nanoparticles for extrahepatic biliary duct visualization in liver cirrhosis

Contact Info

Product

Resources

About