Superparamagnetic Iron Oxide–Enhanced Magnetic Resonance Imaging of Neuroinflammation in a Rat Model of Radicular Pain

Thorek, Daniel L.J.; Weisshaar, Christine L.; Czupryna, Julie; Winkelstein, Beth A.; Tsourkas, Andrew

doi:10.2310/7290.2010.00042

Cited by 17 publications

(24 citation statements)

References 52 publications

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“…An extensive number of studies have evaluated the biocompatibility of SPIONs in a variety of cells (e.g., fibroblast, T cells, keratinocytes, and macrophages) [24][25][26] and a number of animal studies (e.g., radicular pain and mammary adenocarcinomas). 12,27 However, there is only limited information available about the evaluation of cellular responses within human tumor tissues. Therefore, the goal of this study was to evaluate the in vitro cellular responses of USPIONs with cells from human breast tissue.…”

Section: Discussionmentioning

confidence: 99%

Effects of iron oxide nanoparticles on biological responses and MR imaging properties in human mammary healthy and breast cancer epithelial cells

et al. 2015

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Superparamagnetic iron oxide nanoparticles (SPIONs, diameters >50 nm) have received great attention due to their promising use as magnetic resonance imaging (MRI) contrast agents. In this study, we evaluated the cellular uptake and biological responses in vitro of ultrasmall SPIONs (USPIONs, diameters < 50 nm). We compared the cellular responses between breast epithelia isolated from healthy and breast cancer donors after exposure to carboxy-terminated USPIONs (10 and 30 nm PEG-coated, 10 and 30 nm non-PEG-coated). The particles were characterized using transmission electron microscopy (TEM), dynamic light scattering (DLS) and gel electrophoresis. Cellular interactions with USPIONs were assessed by confocal microscopy and TEM. Cellular uptake of USPIONs was quantified using ICP-MS. Cell viability was measured by MTT and neutral red uptake assays. T2* weighted MRI scans were performed using a 7T scanner. Results demonstrated that cell association/internalization of USPIONs was size- and surface coating-dependent (PEG vs. non-PEG), and higher cellular uptake of 10 and 30 nm non-coated particles was observed in both cell types compared with PEG-coated particles. Cell uptake for 10 and 30 nm non-coated particles was higher in cancer cells from two of three tested donors compared to healthy cells from three donors. There was no significant cytotoxicity observed for all tested particles. Significantly enhanced MRI contrast was observed following exposure to 10 and 30 nm non-coated particles compared to PEG-coated particles in both cell types. In comparison, cancer cells showed more enhanced MRI signals when compared to normal cells. The data indicate that cell responses following exposure to USPIONs are dependent on particle properties. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1032-1042, 2016.

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Section: Discussionmentioning

confidence: 99%

Effects of iron oxide nanoparticles on biological responses and MR imaging properties in human mammary healthy and breast cancer epithelial cells

et al. 2015

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“…[32] Immediately after compression, either 100 µl (137 U/mL) of SOD-loaded porous polymersomes in saline, empty polymersomes (no SOD encapsulation), or free SOD at either a comparable dose (137 U/mL) or higher dose (17505 U/mL) of free SOD in saline was administered directly to the nerve root. As shown in Figure 3a, administration of antioxidant polymersomes prevents the development of pain that is typically induced by a nerve root compression.…”

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confidence: 99%

Superoxide Dismutase‐Loaded Porous Polymersomes as Highly Efficient Antioxidants for Treating Neuropathic Pain

Kartha

Yan

Weisshaar

et al. 2017

Adv Healthcare Materials

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Highly efficient antioxidants based on superoxide dismutase (SOD) -loaded porous polymersomes are developed for treating neuropathic pain. The SOD-loaded porous polymersomes are highly permeable to superoxide radical, while retaining the antioxidant enzyme within their aqueous interiors. Administration of the antioxidant porous polymersomes following a painful nerve root compression is substantially more effective in preventing the onset of pain in rats than comparable or higher doses of free SOD alone.

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“…MRI has been used to image nanoparticle distribution in a variety of contexts, such as neuroinflammation in root compression (28), soft tissue infection (29), atherosclerosis (30, 31), and angiogenesis in tumor models (32, 33), as well as to confirm SPIO delivery to rabbit liver tumors (8, 34). Recent work in a different rodent model of HCC showed that MRI can quantify both intratumoral uptake of SPIOs after intravenous delivery (17) and labeled NK cells after intra-arterial delivery (35).…”

Section: Discussionmentioning

confidence: 99%

MR Imaging Enables Measurement of Therapeutic Nanoparticle Uptake in Rat N1-S1 Liver Tumors after Nanoablation

McDevitt

Mouli

Tyler

et al. 2014

Journal of Vascular and Interventional Radiology

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Purpose Superparamagnetic iron oxide nanoparticles (SPIOs) functionalized with doxorubicin (DOX) can serve dual diagnostic and therapeutic purposes. Nanoablation is an approach to increase intratumoral nanoparticle uptake that combines IV nanoparticle delivery with reversible electroporation. However, a method to quantify drug delivery during this therapy is needed. This study tested the hypothesis that MRI can quantify intratumoral SPIO uptake after nanoablation. Methods DOX-SPIOs were synthesized. N1-S1 hepatomas were successfully induced in 17 Sprague-Dawley rats distributed into three dosage groups. Baseline tumor R2* values (the reciprocal of T2*) were determined using 7T MRI. Following IV injection of SPIOs, reversible electroporation (1300 V/cm, 8 pulses, 100 µs pulse duration) was applied. Animals were imaged to determine post-procedural tumor R2* and change in R2* (ΔR2*) was calculated. Inductively-coupled plasma mass spectrometry was used to determine post-procedure intratumoral iron concentration, which served as a proxy for SPIO uptake. Mean tumor iron concentration and ΔR2* for each subject were assessed for correlation with linear regression, and mean iron concentration for each dosage group was compared with analysis of variance. Results ΔR2* significantly correlated with tumor SPIO uptake after nanoablation (r=0.50, p=0.039). On average, each 0.1 ms−1 increase in R2* corresponded to a 0.1394 mM increase in iron concentration. There was no significant difference in mean SPIO uptake among dosage groups (p=0.57). Conclusion Intratumoral SPIO uptake after nanoablation can be successfully quantified non-invasively with 7T MRI. Imaging can thus be used as a method to estimate localized drug delivery after nanoablation.

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Superparamagnetic Iron Oxide–Enhanced Magnetic Resonance Imaging of Neuroinflammation in a Rat Model of Radicular Pain

Cited by 17 publications

References 52 publications

Effects of iron oxide nanoparticles on biological responses and MR imaging properties in human mammary healthy and breast cancer epithelial cells

Effects of iron oxide nanoparticles on biological responses and MR imaging properties in human mammary healthy and breast cancer epithelial cells

Superoxide Dismutase‐Loaded Porous Polymersomes as Highly Efficient Antioxidants for Treating Neuropathic Pain

MR Imaging Enables Measurement of Therapeutic Nanoparticle Uptake in Rat N1-S1 Liver Tumors after Nanoablation

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