Superoxide generation by pig alveolar macrophages

Zeidler, Robert; Conley, Nick

doi:10.1016/0305-0491(86)90228-2

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…We were unable to find the specific parameters and that determine the kinetics with which Akt phosphorylates Raf in macrophages. Instead we estimate these parameters to be equal to the parameters that determine the kinetics of the reaction between Akt and a small peptide substrate [101] under the assumptions that the concentration of ATP in a resting pig alveolar macrophage is equal to that of resting human alveolar macrophage [102], and that the volume of a pig alveolar macrophage is [103]. The parameters which determine the rate of Raf activation by Lyn and Pak are also unknown.…”

Section: Methodsmentioning

confidence: 99%

A Mathematical Model of CR3/TLR2 Crosstalk in the Context of Francisella tularensis Infection

et al. 2012

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Complement Receptor 3 (CR3) and Toll-like Receptor 2 (TLR2) are pattern recognition receptors expressed on the surface of human macrophages. Although these receptors are essential components for recognition by the innate immune system, pathogen coordinated crosstalk between them can suppress the production of protective cytokines and promote infection. Recognition of the virulent Schu S4 strain of the intracellular pathogen Francisella tularensis by host macrophages involves CR3/TLR2 crosstalk. Although experimental data provide evidence that Lyn kinase and PI3K are essential components of the CR3 pathway that influences TLR2 activity, additional responsible upstream signaling components remain unknown. In this paper we construct a mathematical model of CR3 and TLR2 signaling in response to F. tularensis. After demonstrating that the model is consistent with experimental results we perform numerical simulations to evaluate the contributions that Akt and Ras-GAP make to ERK inhibition. The model confirms that phagocytosis-associated changes in the composition of the cell membrane can inhibit ERK activity and predicts that Akt and Ras-GAP synergize to inhibit ERK.

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Section: Methodsmentioning

confidence: 99%

A Mathematical Model of CR3/TLR2 Crosstalk in the Context of Francisella tularensis Infection

et al. 2012

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Subpopulation of alveolar macrophages inhibits superoxide anion production by macrophages

et al. 1987

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Pig alveolar macrophages are a heterogeneous population of cells. Three subpopulations or bands exist when the whole population is separated according to density. Band 1 cells are the least dense cells and constitute 9% of the total population. Bands 2 and 3 represent 44 and 47% of the total population. The three subpopulations generate superoxide anions, although to varying degrees. Band 3 cells are the most active, while band 1 cells are the least active. The amount of superoxide anions released in a mixed population of bands 1, 2, and 3 cells was less than the sum of that produced from each band assayed separately. Band 1 cells were found to inhibit by 47% the production of superoxide anions by band 3 cells. Conditioned medium from band 1 cells contains a heat-sensitive, nondialyzable, soluble factor responsible for this inhibition.

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Superoxide generation by pig alveolar macrophages

Cited by 2 publications

References 17 publications

A Mathematical Model of CR3/TLR2 Crosstalk in the Context of Francisella tularensis Infection

A Mathematical Model of CR3/TLR2 Crosstalk in the Context of Francisella tularensis Infection

Subpopulation of alveolar macrophages inhibits superoxide anion production by macrophages

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