Superoxide Dismutases in Polymorphonuclear Leukocytes

Salin, Marvin L.; McCord, Joe M.

doi:10.1172/jci107816

Cited by 254 publications

(71 citation statements)

References 17 publications

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“…Like hydrogen peroxide production, superoxide production is defective in neutrophils from patients with chronic granulomatous disease (51). Superoxide dismutase may be present in phagocytic vacuoles, because it has been localized to the cytoplasm of neutrophils (52), and may then play a significant role in catalyzing the formation of hydrogen peroxide from superoxide (49). However, in addition to the phagocytic vacuole, superoxide production appears to take place on the outer surface of the cell membrane (52,53).…”

Section: Discussionmentioning

confidence: 99%

“…Superoxide dismutase may be present in phagocytic vacuoles, because it has been localized to the cytoplasm of neutrophils (52), and may then play a significant role in catalyzing the formation of hydrogen peroxide from superoxide (49). However, in addition to the phagocytic vacuole, superoxide production appears to take place on the outer surface of the cell membrane (52,53). In fact, superoxide may be produced by neutrophils after exposure to certain stimuli in the absence of phagocytosis (54).…”

Section: Discussionmentioning

confidence: 99%

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Mechanisms of Attachment of Neutrophils to Candida albicans Pseudohyphae in the Absence of Serum, and of Subsequent Damage to Pseudohyphae by Microbicidal Processes of Neutrophils In Vitro

Daimond¹,

Krzesicki²

1978

J. Clin. Invest.

121

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A B S T R A C T Mechanisms were studied that might explain the attachment and damage to Candida albicans pseudohyphae by neutrophils in the absence of serum. Attachment of neutrophils to pseudo hyphae was inhibited by Candida mannans (1-10 mg/ml), but not by mannose, dextran, chitin, conconavalin A, or highly charged polyamino acids. Contact was also inhibited by pretreatment of Candida before incubation with neutrophils with chymotrypsin, but not trypsin or several inhibitors of proteases. Similar results were obtained with pretreatment of neutrophils, except that trypsin was inhibitory. When pseudohyphae were killed with ultraviolet light, proteinpolysaccharide complexes of mol wt <10,000 were released which appeared to bind to the surfaces of neutrophils and inhibit contact between neutrophils and Candida, as well as other fungi.Damage to Candida by neutrophils was inhibited by agents known to act on neutrophil oxidative microbicidal mechanisms, including sodium cyanide, sodium azide, catalase, superoxide dismutase, and 1, 4 diazobicyclo (2, 2, 2) octane, a singlet oxygen quencher. Neutrophils from a patient with chronic granulomatous disease did not damage Candida at all. However, the hydroxyl radical scavengers mannitol and benzoate were not inhibitory. Cationic proteins and lactoferrin also did not appear to play a major role in this system. Low concentrations of lysozyme which Preliminary findings were presented in part at the Annual

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Mechanisms of Attachment of Neutrophils to Candida albicans Pseudohyphae in the Absence of Serum, and of Subsequent Damage to Pseudohyphae by Microbicidal Processes of Neutrophils In Vitro

Daimond¹,

Krzesicki²

1978

J. Clin. Invest.

121

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“…Overwhelming radicals generated in the blood stream and tissues can induce oxidative damage to cell membranes, which contain lipoproteins, proteins and deoxyribonucleic acid [32]. The present study suggests that, Raphnus sativus partial purified protein with SOD-like activity may be one of the treatment strategies.…”

Section: Discussionmentioning

confidence: 62%

“…Malondialdehyde (MDA) was determined in the blood by the method of [31]. Superoxide dismutase (SOD) activity in serum was assayed by the method of [32]. Blood hemoglobin (Hb) concentration was colourimetrically determined according to the method of [33].…”

Section: Biochemical Testsmentioning

confidence: 99%

Suppression Effect of Superoxide Dismutase (SOD)-Like Activity Protein Partially Purified from <i>Raphnus sativus</i> Leaves against Liver Metastasis in Mice Intraperitoneally Infected with Ehrlich Ascites Carcinoma Cell

Toson¹,

Almutairi²,

Elfalal³

et al. 2016

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Liver metastasis is a normal result in Ehrlich Ascites Carcinoma Cell (EAC) infected mice. A partially purified protein with Superoxide Dismutase (SOD)-like activity extracted from leaves of Raphnus sativus was tested for its ability to down regulate liver metastasis of Ehrlich Ascites Carcinoma Cell (EAC) intraperitoneally implanted in albino mice. Sixty albino mice were divided equally into 4 groups. Group I mice (G1 control) were treated with physiologic saline solution for 7 days. Group 2 mice (G2) were injected with the partially purified protein. Group 3 mice (G3) were intraperitoneally inoculated with EAC cells and treated with the partially purified protein. Group 4 mice (G4) were inoculated EAC cells and treated with physiologic saline solution. The results showed a disturbance in liver functions. This disturbance was observed as a reduction in the values of serum albumin, hemoglobin, total counts of erythrocytes and platelets, total lipids in liver tissues, erythrocytes SOD activity, serum glucose, reduced glutathione (GSH) and the trace elements (Fe, Mn, Zn, and Cu) contents in liver and spleen tissues as well as the elevation of serum transaminases activities, the levels of malondialdehyde in blood, DNA and RNA of liver tissues in the infected mice (G4). On the other hand, in G3, after treatment with the partial purified protein all these parameters restored their normal values and became near from the normal group (GI). Moreover, the protein showed no toxicity towards the mice as shown in G2. It could be concluded 322that a partially purified protein with Superoxide Dismutase (SOD)-like activity extracted from leaves of Raphnus sativus as a nature source has a promised future in controlling liver metastasis.

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“…In contrast, the intracellularly generated H202, if any, could not be similarly excluded. However, the cell should be protected against any H202 toxicity by cytosol peroxidases (Salin & McCord, 1974).…”

Section: Rsh+h+±+02--+rsmentioning

confidence: 99%

Oxygen-dependent free radicals in spermine oxidation cytostasis and chemiluminescence and the role of superoxide dismutase

Gaugas¹,

Dewey²

1980

Br J Cancer

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Summary.-Spermine interacted with serum polyamine oxidase (PAO) to arrest proliferation of cultured Bri8 lymphocytes. Arrest was independent of catalase activity and was not directly due to an H202 byproduct. Arrest was averted by 3-hydroxybenzyloxyamine, which inactivates the pyridoxal co-factor of PAO. The oxidation of spermine in the presence of different concentrations of PAO was nonlinear, which implied complex intermediate events for conversion of spermine to labile di-oxidized spermine (N,N'-bis(3-propionaldehyde)-1,4-butanediamine) with, perhaps, overall generation of free radicals (02-and-OH) which are damaging to cells. Exogenous free radicals were apparently neither direct participants in cytostasis, nor in the chemiluminescence demonstrable for spermine oxidation. Thiourea, an 'OH scavenger, protected against both proliferation arrest and luminescence. Many other powerful OH scavengers, however, were ineffective. Though reaction mixtures reduced ferricytochrome c initially, reduction was not inhibited by superoxide dismutase (SOD) which indicated that the anion 02-had not been generated. The powerful reducing capability of di-oxidized spermine itself could have competed against any 02-for ferricytochrome c reduction. Nevertheless, 02-was generated during further PAO conversion and/or auto-oxidation of di-oxidized spermine. Curiously, addition of SOD to destroy presumptive 02-variably potentiated cytotoxicity. Blockage of any anion channels in the cell plasma membrane by stilbene derivatives did not influence cytotoxicity. Thus, findings support our previous evidence that cationic di-oxidized spermine is a potent G1 inhibitor of cell proliferation. The possibility of intracellular free-radical and thiol involvement is discussed.

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Superoxide Dismutases in Polymorphonuclear Leukocytes

Cited by 254 publications

References 17 publications

Mechanisms of Attachment of Neutrophils to Candida albicans Pseudohyphae in the Absence of Serum, and of Subsequent Damage to Pseudohyphae by Microbicidal Processes of Neutrophils In Vitro

Mechanisms of Attachment of Neutrophils to Candida albicans Pseudohyphae in the Absence of Serum, and of Subsequent Damage to Pseudohyphae by Microbicidal Processes of Neutrophils In Vitro

Suppression Effect of Superoxide Dismutase (SOD)-Like Activity Protein Partially Purified from <i>Raphnus sativus</i> Leaves against Liver Metastasis in Mice Intraperitoneally Infected with Ehrlich Ascites Carcinoma Cell

Oxygen-dependent free radicals in spermine oxidation cytostasis and chemiluminescence and the role of superoxide dismutase

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Superoxide Dismutases in Polymorphonuclear Leukocytes

Cited by 254 publications

References 17 publications

Mechanisms of Attachment of Neutrophils to Candida albicans Pseudohyphae in the Absence of Serum, and of Subsequent Damage to Pseudohyphae by Microbicidal Processes of Neutrophils In Vitro

Mechanisms of Attachment of Neutrophils to Candida albicans Pseudohyphae in the Absence of Serum, and of Subsequent Damage to Pseudohyphae by Microbicidal Processes of Neutrophils In Vitro

Suppression Effect of Superoxide Dismutase (SOD)-Like Activity Protein Partially Purified from &lt;i&gt;Raphnus sativus&lt;/i&gt; Leaves against Liver Metastasis in Mice Intraperitoneally Infected with Ehrlich Ascites Carcinoma Cell

Oxygen-dependent free radicals in spermine oxidation cytostasis and chemiluminescence and the role of superoxide dismutase

Contact Info

Product

Resources

About

Suppression Effect of Superoxide Dismutase (SOD)-Like Activity Protein Partially Purified from <i>Raphnus sativus</i> Leaves against Liver Metastasis in Mice Intraperitoneally Infected with Ehrlich Ascites Carcinoma Cell