Superoxide Dismutases

Purification, molecular cloning, and some properties of a manganese-containing superoxide dismutase from Japanese flounder (Paralichthys olivaceus)

“…The oxygen-induced cell injury in mitochondria includes the increased production of superoxide anion, mitochondrial injury, and decreased ATP production, which result in cell dysfunction and death (Crapo et al, 1980;Hyslop et al, 1988;Schoonen et al, 1990). Mn-SOD, a mitochondrial protein, has the ability to catalyse the toxic superoxide anion into molecular oxygen and hydrogen peroxide (McCord and Fridovich, 1969), then hydrogen peroxide will be removed by catalase and glutathione peroxidase (Beyer et al, 1991;Whittaker, 2000).…”

“…Among the enzymatic system, superoxide dismutase (SOD; EC 1.15.1.1) is a family of important antioxidant metalloenzymes. It catalyzes the dismutation of toxic superoxide anion into dioxygen and hydrogen peroxide (McCord and Fridovich, 1969;Whittaker, 2000), which is subsequently reduced to water by glutathione peroxidase in the cytosol, or by catalase in the peroxisomes (Beyer et al, 1991;Whittaker, 2000;Hermes-Lima and Zenteno-Savın, 2002). Increasing evidence has shown that SOD is related to numerous physiological and pathological situations, such as aging, tumors, autoimmunity diseases and radioprotection (Oberley and Buettner, 1979;Warner, 1994;Lih-Brody et al, 1996;Zhang et al, 2008), so it has fine foreground of development and application.…”

Novel Insights into the Basis for Escherichia coli Superoxide Dismutase's Metal Ion Specificity from Mn-Substituted FeSOD and Its Very High Em

“…However, consistent with the seminal work of Yamakura (20,38) Mn(Fe)SOD is completely inactive in the standard assay ( Table 1). The somewhat variable substoichiometric binding of Mn lowers the spectroscopic signal intensities obtained but should not affect the nature of the signals observed or the activity of individual sites since MnSOD activity is directly proportional to the fraction of sites occupied by Mn (39). Figure 2 compares the EPR spectrum of Mn 2+ (Fe)SOD with that of Mn 2+ SOD.…”

Superoxide dismutase and catalase anti-oxidant activity in leucocyte lysates from hypertensive patients: effects of eprosartan treatment

“…This mechanism means that both enzymes constitute an important defence mechanism against oxidative stress. 3 Various types of SODs have been described, 4 and all provide an essential defence system for live cells. 5 CAT uses hydrogen peroxide as a source of electrons and is found in almost all eucaryotic cells, located in peroxisomes.…”