Superoxide dismutase, glutathione peroxidase activities and the hydroperoxide concentration are modified in the hippocampus of epileptic rats

Bellissimo, Maria Ines; Amado, Débora; Abdalla, Dulcinéia Saes Parra; Ferreira, Edmar Cid; Cavalheiro, Ésper A.; Naffah-Mazzacoratti, Maria da Graça

doi:10.1016/s0920-1211(01)00269-8

Cited by 139 publications

(54 citation statements)

References 32 publications

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“…In a pilocarpine model, SOD and CAT activities were increased at 2 h and 60 d after SE, respectively, indicating that endogenous antioxidant reagents are up-regulated due to increased oxidant radicals (Freitas, 2009). However, other reports have demonstrated no change or even a decrease in SOD activity (Bellissimo et al, 2001;Freitas et al, 2005). This discrepancy may result from differences in the animal models, severity of injury, selected time points and/or methods of detection.…”

Section: Ka-induced Se and Oxidative Stressmentioning

confidence: 88%

Neuroprotective effects of flavonoids extracted from licorice on kainate-induced seizure in mice through their antioxidant properties

Zeng

Zhang

et al. 2013

J. Zhejiang Univ. Sci. B

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Abstract:A relationship between status epilepticus (SE) and oxidative stress has recently begun to be recognized. To explore whether the flavonoids extracted from licorice (LFs) have any protective effect on kainate (KA)-induced seizure in mice, we treated mice with LFs before and after KA injection. In KA-treated mice, we found that superoxide dismutase (SOD) activity decreased immediately after the onset of seizure at 1 h and then increased at 6 h. It returned to baseline 1 d after seizure and then increased again at 3, 7, and 28 d, while malondialdehyde (MDA) content remained at a high level at 1 h, 6 h, 3 d, 7 d, and 28 d, indicating a more oxidized status related to the presence of more reactive oxygen species (ROS). Treatment with LFs before KA injection reversed the seizure-induced change in SOD activity and MDA content at 1 h, 6 h, 3 d, 7 d, and 28 d. Treatment with LFs after seizure decreased KA-induced SOD activity and MDA content at 7 and 28 d. Also, LF pre-and post-KA treatments decreased seizure-induced neuronal cell death. Subsequently, Morris water maze tests revealed that the escape latency was significantly decreased and the number of target quadrant crossings was markedly increased in the LF-treated groups. Thus, our data indicate that LFs have protective effects on seizure-induced neuronal cell death and cognitive impairment through their anti-oxidative effects.

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Section: Ka-induced Se and Oxidative Stressmentioning

confidence: 88%

Neuroprotective effects of flavonoids extracted from licorice on kainate-induced seizure in mice through their antioxidant properties

Zeng

Zhang

et al. 2013

J. Zhejiang Univ. Sci. B

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“…[26,27] Researchers have observed contradictory results in the levels of SOD, either in acute or chronic models. [28][29][30] SOD is one of the major antioxidants. In current study, SOD levels increased in doxycycline treated when compared with saline-given.…”

Section: Discussionmentioning

confidence: 99%

Presentation to Affirmative Effect of Doxcycyline in pentylene tetrazole induced Seizures models

Erbaş¹,

Çelik²,

Cinar³

et al. 2014

Epilepsi

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Amaç: Daha önce yapılan çalışmalarda enflamasyonun epilepsi patogeneziyle olan ilişkisi gösterilmiştir, buna göre oksidatif stresin de epilepsi patogeneziyle ilişkili olabileceği düşünülebilir. Literatürde doksisiklinin hem antienflamatuvar hem de antioksidan etkilerini inceleyen çalışmalar bulunmaktadır. Bu çalışmanın amacı doksisiklinin muhtemel antikonvulzan etkilerini elektroensefelografi kayıtları (EEG), laboratuvar bulguları ve davranışsal bulgular eşliğinde incelemektir. Gereç ve Yöntem:Otuz altı sıçan rastgele iki gruba ayrıldı. Grup A EEG kayıtları, Group B ise davranışsal değişikliklerin incelenmesi için belirlendi. 35 mg/kg pentilentetrazol (PTZ) EEG kayıtları için, 70 mg/kg PTZ davranışsal değişiklikler oluşturmak için kullanıldı. Davranışsal değişikliklerin değerlendirilmesinde ilk miyoklonik jerk zamanı ve Racine konvulziyon skalası kullanıldı, EEG kayıtlarının değerlendirilmesinde ise spike yüzdeleri kullanıldı. Bulgular:Yapılan istatistiksel analiz sonucunda doksisiklin verilen gruplarda spike yüzdeleri, ilk miyoklonik jerk zamanları ve Racine konvulziyon skoları açısından doksisiklinin olumlu etkileri tespit edildi. Ayrıca çalışmamızda doksisiklin verilen grupta malonedialdehit seviyeleri düşmüşken, süperoksitdismutaz seviyelerinin artmış olduğu görüldü.Sonuç: Sunulan çalışmada doksisiklinin PTZ indükte konvulziyonlar üzerine olumlu etkileri olduğu görülmüştür, bu etkisini de antienflamatuvar ve nöroprotektif özellikler yoluyla olabileceğini düşünmekteyiz. SummaryObjectives: There were some experimental and clinical evidence that inflammation in the brain is likely to predispose epileptogenesis. Also, it is thought to be that oxidative stress can play a role in epilepsy. Both laboratory and clinical studies have demonstrated the antiinflammatory-antioxidant properties of doxycycline. We aimed to highlight the anticonvulsant action of doxycycline based on clinical, laboratory and EEG findings in animal models. Methods: 36 rats were randomly divided in two groups. Group A for EEG recordings and Group B for behavioral assesment. 35 mg/kg pentylene tetrazole (PTZ) used for EEG recording and 70 mg/kg PTZ used for behavioral evaulations. For behavioral evaluations we assessed first myoclonic jerk time (FMJ) and Racine convulsion scores (RCS). Results:The groups were evaluated according to EEG records and severety of seizures. It was found to be doxcycyline is effective both on the time of the first myoclonic jerk' (FMJ) latency and Racine convulsion scale (RCS) scores. Besides, doxcycyline significantly decreased to the percentage of spikes on electroencephalography (EEG) records. Also In current study, it was detected that Malone dialdehit (MDA) levels decreased and superoxide dismutase (SOD) activity increased in doxcycyline-treated groups. Conclusion:In our study, we evaluated that the anticonvulsant effects of doxycycline showed a dose dependent protective effect against PTZ-induced seizures in rats by its anti-inflammatory and neuroprotective properties.

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“…MPO can inactivate inhibitors of matrix metalloproteinases (MMP) (7) and activate MMP (8) to break down the blood-brain barrier (BBB), and BBB leakage is implicated in seizure induction and progression (9). The brain is particularly susceptible to oxidative stress, since it contains large quantities of oxidizable lipids and metals and has fewer antioxidant mechanisms than other tissues (10). During epileptogenesis, there is a marked increase in oxidative stress (11,12).…”

Section: Seizure Inductionmentioning

confidence: 99%

“…During epileptogenesis, there is a marked increase in oxidative stress (11,12). Moreover, increased levels of hydrogen peroxide, a critical substrate for MPO, are found in mice brains during epileptogenesis (10).…”

Section: Seizure Inductionmentioning

confidence: 99%

Myeloperoxidase Nuclear Imaging for Epileptogenesis

Zhang¹,

Seeburg²,

Pulli³

et al. 2017

Radiology

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q RSNA, 2015 Purpose:To determine if myeloperoxidase (MPO) is involved in epileptogenesis and if molecular nuclear imaging can be used to noninvasively map inflammatory changes in epileptogenesis. Materials and Methods:The animal and human studies were approved by the institutional review boards. Pilocarpine-induced epileptic mice were treated with 4-aminobenzoic acid hydrazide (n = 46), a specific irreversible MPO inhibitor, or saline (n = 42). Indium-111-bis-5-hydroxytryptamide-diethylenetriaminepentaacetate was used to image brain MPO activity (n = 6 in the 4-aminobenzoic acid hydrazide and saline groups; n = 5 in the sham group) by using single photon emission computed tomography/computed tomography. The role of MPO in the development of spontaneous recurrent seizures was assessed by means of clinical symptoms and biochemical and histopathologic data. Human brain specimens from a patient with epilepsy and a patient without epilepsy were stained for MPO. The Student t test, one-way analysis of variance, and Mann-Whitney and Kruskal-Wallis tests were used. Differences were regarded as significant if P was less than .05. Results:MPO and leukocytes increased in the brain during epileptogenesis (P , .05). Blocking MPO delayed spontaneous recurrent seizures (99.6 vs 142 hours, P = .016), ameliorated the severity of spontaneous recurrent seizures (P , .05), and inhibited mossy fiber sprouting (Timm index, 0.31 vs 0.03; P = .003). Matrix metalloproteinase activity was upregulated during epileptogenesis in an MPO-dependent manner (1.44 vs 0.94 U/mg, P = .049), suggesting that MPO acts upstream of matrix metalloproteinases. MPO activity was mapped during epileptogenesis in vivo in the hippocampal regions. Resected temporal lobe tissue from a human patient with refractory epilepsy but not the temporal lobe tissue from a patient without seizures demonstrated positive MPO immunostaining, suggesting high translational potential for this imaging technology. Conclusion:The findings of this study highlight an important role for MPO in epileptogenesis and show MPO to be a potential therapeutic target and imaging biomarker for epilepsy.q RSNA, 2015

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Superoxide dismutase, glutathione peroxidase activities and the hydroperoxide concentration are modified in the hippocampus of epileptic rats

Cited by 139 publications

References 32 publications

Neuroprotective effects of flavonoids extracted from licorice on kainate-induced seizure in mice through their antioxidant properties

Neuroprotective effects of flavonoids extracted from licorice on kainate-induced seizure in mice through their antioxidant properties

Presentation to Affirmative Effect of Doxcycyline in pentylene tetrazole induced Seizures models

Myeloperoxidase Nuclear Imaging for Epileptogenesis

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