Superoxide dismutase, catalase, and glutathione peroxidase activities in muscle and lymphoid organs of sedentary and exercise-trained rats

Pereira, Benedito; Rosa, L. De; Safi, D A; Medeiros, Marisa H. G.; Curi, Rui; Bechara, Etelvino J.H.

doi:10.1016/0031-9384(94)90349-2

Cited by 50 publications

(43 citation statements)

References 20 publications

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“…In situations where H 2 O 2 is significantly elevated, CAT becomes an important H 2 O 2 reducing system, and its enzymatic activity prevails because there is no apparent V max and cannot be saturated by H 2 O 2 at any concentration 136. CAT enzymatic activity is higher in oxidative myofibres compared with fast glycolytic fibres137 and does not require reducing equivalents to function as a H 2 O 2 reducer; thus, CAT is considered an energy‐efficient enzyme 138…”

Section: Regulatory Reactive Oxygen and Nitrogen Species Enzymes Exprmentioning

confidence: 99%

Redox homeostasis and age‐related deficits in neuromuscular integrity and function

Sakellariou

Lightfoot

Earl

et al. 2017

J cachexia sarcopenia muscle

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Skeletal muscle is a major site of metabolic activity and is the most abundant tissue in the human body. Age‐related muscle atrophy (sarcopenia) and weakness, characterized by progressive loss of lean muscle mass and function, is a major contributor to morbidity and has a profound effect on the quality of life of older people. With a continuously growing older population (estimated 2 billion of people aged >60 by 2050), demand for medical and social care due to functional deficits, associated with neuromuscular ageing, will inevitably increase. Despite the importance of this ‘epidemic’ problem, the primary biochemical and molecular mechanisms underlying age‐related deficits in neuromuscular integrity and function have not been fully determined. Skeletal muscle generates reactive oxygen and nitrogen species (RONS) from a variety of subcellular sources, and age‐associated oxidative damage has been suggested to be a major factor contributing to the initiation and progression of muscle atrophy inherent with ageing. RONS can modulate a variety of intracellular signal transduction processes, and disruption of these events over time due to altered redox control has been proposed as an underlying mechanism of ageing. The role of oxidants in ageing has been extensively examined in different model organisms that have undergone genetic manipulations with inconsistent findings. Transgenic and knockout rodent studies have provided insight into the function of RONS regulatory systems in neuromuscular ageing. This review summarizes almost 30 years of research in the field of redox homeostasis and muscle ageing, providing a detailed discussion of the experimental approaches that have been undertaken in murine models to examine the role of redox regulation in age‐related muscle atrophy and weakness.

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Section: Regulatory Reactive Oxygen and Nitrogen Species Enzymes Exprmentioning

confidence: 99%

Redox homeostasis and age‐related deficits in neuromuscular integrity and function

Sakellariou

Lightfoot

Earl

et al. 2017

J cachexia sarcopenia muscle

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show abstract

“…De fato, os sítios formadores de EROs encontram-se elevados no interior das mitocôndrias dos músculos de animais treinados em resistência devido a proliferação mitocondrial promovida por esta m odalidade de treinamento físico (Benzi, 1993, Booth & Thomason, 1991, Holloszy, 1975. Demonstramos recentemente que o treinam ento físico de resistência aeróbica em natação promove aumento da atividade da enzim a citrato sintetase e M n-SOD do músculo sóleo de ratos (Pereira et alii, 1994c). Portanto, os dados referentes ao aumento da atividade destas enzimas, ambas mitocondriais, indicam que o treinam ento físico de resistência promove efeito adaptativo predom inantemente mitocondrial nas fibras musculares verm elhas do músculo sóleo dos ratos treinados em exercício de resistência aeróbica (Booth & Thomason, 1991, Pereira et alii, 1994c.…”

Section: Efeito S Do Ex Er C íC Io Físico So Bre As Defesas a N T Io unclassified

“…Demonstramos recentemente que o treinam ento físico de resistência aeróbica em natação promove aumento da atividade da enzim a citrato sintetase e M n-SOD do músculo sóleo de ratos (Pereira et alii, 1994c). Portanto, os dados referentes ao aumento da atividade destas enzimas, ambas mitocondriais, indicam que o treinam ento físico de resistência promove efeito adaptativo predom inantemente mitocondrial nas fibras musculares verm elhas do músculo sóleo dos ratos treinados em exercício de resistência aeróbica (Booth & Thomason, 1991, Pereira et alii, 1994c. Em paralelo, constatamos menor atividade da glutationa peroxidase nos músculos sóleo e gastrocnêmio com o treinam ento de resistência imposto.…”

Section: Efeito S Do Ex Er C íC Io Físico So Bre As Defesas a N T Io unclassified

“…Estes dados estão de acordo com os descritos por Benzi (1993). Ou seja, as fibras musculares demonstram alta produção de oxi-radicais e peróxido de hidrogênio e baixa capacidade antioxidante enzimática (Benzi, 1993, Pereira et alii, 1994c. Isto levaria, como conseqüência, ao incremento da peroxidação lipídica detectada nestas fibras musculares em conseqüência do treinam ento físico com exercício intenso (Benzi, 1993, Pereira et alii, 1994c.…”

Section: Efeito S Do Ex Er C íC Io Físico So Bre As Defesas a N T Io unclassified

“…Ou seja, as fibras musculares demonstram alta produção de oxi-radicais e peróxido de hidrogênio e baixa capacidade antioxidante enzimática (Benzi, 1993, Pereira et alii, 1994c. Isto levaria, como conseqüência, ao incremento da peroxidação lipídica detectada nestas fibras musculares em conseqüência do treinam ento físico com exercício intenso (Benzi, 1993, Pereira et alii, 1994c. Os resultados conflitantes referentes as enzim as superóxido dismutases, catalase e glutationa peroxidase podem ser explicados, em parte, pelas diferentes espécies anim ais estudadas (camundongos vs ratos vs cavalos), diferentes intensidades do exercício físico empregado e diferentes procedimentos técnicos utilizados no ensaio destas enzim as (Jenkins, 1988).…”

Section: Efeito S Do Ex Er C íC Io Físico So Bre As Defesas a N T Io unclassified

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Physical exercise as pro-oxidant

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The impact of antioxidant supplements and endurance exercise on genes of the carbohydrate and lipid metabolism in skeletal muscle of mice

Meier

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To ascertain whether reactive oxygen species (ROS) contribute to training-induced adaptation of skeletal muscle, we administered ROS-scavenging antioxidants (AOX; 140 mg/l of ascorbic acid, 12 mg/l of coenzyme Q10 and 1% N-acetyl-cysteine) via drinking water to 16 C57BL/6 mice. Sixteen other mice received unadulterated tap water (CON). One cohort of both groups (CON(EXE) and AOX(EXE) ) was subjected to treadmill exercise for 4 weeks (16-26 m/min, incline of 5°-10°). The other two cohorts (CON(SED) and AOX(SED) ) remained sedentary. In skeletal muscles of the AOX(EXE) mice, GSSG and the expression levels of SOD-1 and PRDX-6 were significantly lower than those in the CON(EXE) mice after training, suggesting disturbance of ROS levels. The peak power related to the body weight and citrate synthase activity was not significantly influenced in mice receiving AOX. Supplementation with AOX significantly altered the mRNA levels of the exercise-sensitive genes HK-II, GLUT-4 and SREBF-1c and the regulator gene PGC-1alpha but not G6PDH, glycogenin, FABP-3, MCAD and CD36 in skeletal muscle. Although the administration of AOX during endurance exercise alters the expression of particular genes of the ROS metabolism, it does not influence peak power or generally shift the metabolism, but it modulates the expression of specific genes of the carbohydrate and lipid metabolism and PGC-1alpha within murine skeletal muscle.

show abstract

Superoxide dismutase, catalase, and glutathione peroxidase activities in muscle and lymphoid organs of sedentary and exercise-trained rats

Cited by 50 publications

References 20 publications

Redox homeostasis and age‐related deficits in neuromuscular integrity and function

Redox homeostasis and age‐related deficits in neuromuscular integrity and function

Physical exercise as pro-oxidant

The impact of antioxidant supplements and endurance exercise on genes of the carbohydrate and lipid metabolism in skeletal muscle of mice

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