Superoxide dismutase and cytochrome oxidase activities in light and heavy synaptic mitochondria from rat cerebral cortex during aging

Vanella, A.; Villa, R. F.; Gorini, A.; Campisi, A.; Giuffrida-Stella, Anna Maria

doi:10.1002/jnr.490220316

Cited by 39 publications

(12 citation statements)

References 25 publications

(19 reference statements)

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“…No significant difference among testing groups was observed from the distribution of demographic characteristics analyzed by chi-squared or one-way ANOVA. The concentration of superoxide anions in blood was previously indicated being influenced by age [26,[33][34][35]. Therefore, the subjects over 30 years old were excluded from the test to avoid the effect of age.…”

Section: Resultsmentioning

confidence: 99%

Antioxidative Effect of Far-Infrared Radiation in Human

Lin

Lee²,

Lung³

et al. 2013

PHF

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Abstract-The antioxidative effect of far-infrared radiation (FIR) in human was evaluated by half maximal inhibitory concentration of blood against superoxide anions. All samples ranging from 18 to 30 years old were grouped into sympathetic, parasympathetic, sympathetic plus parasympathetic and the control group. The ability of antioxidation of blood from the subjects was measured with an ultraweak chemiluminescence analyzer. According to the results, the level of superoxide anions was decreased in sympathetic, parasympathetic, and sympathetic plus parasympathetic group, while that in the control group was increased. This suggested that the FIR radiation performed a significantly antioxidative effect by defending human from oxidative damage of superoxide anions in blood. This may probably be achieved by increasing SOD concentration and/or increasing heart rate variability.

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Section: Resultsmentioning

confidence: 99%

Antioxidative Effect of Far-Infrared Radiation in Human

Lin

Lee²,

Lung³

et al. 2013

PHF

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“…4111 H However, disparate findings have been reported in an isolated mitochondrial preparation from aged rat brain. 42 In general. reports demonstrating agedependent increases in superoxide dismutase employed more complete tissue preparations (i.e.…”

Section: Resultsmentioning

confidence: 99%

“…reports demonstrating agedependent increases in superoxide dismutase employed more complete tissue preparations (i.e. whole homogenates), while those in disagreement 42 have essentially only shown data on Mn superoxide dismutase, the less active form of the enzyme. The concentration and distribution of antioxidant enzymes and oxygen radical scavengers ( a-tocopherol.…”

Section: Resultsmentioning

confidence: 99%

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Persistent protein damage despite reduced oxygen radical formation in the aging rat brain

LeBel

Bondy

1991

Intl J of Devlp Neuroscience

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Abstract-The relation between cerebral oxygen radicals and the aging process was investigated in crude synaptosomal (P2) fractions from rats. The rate of formation of oxygen radicals was measured using the probe 2' .7'-dichlorofluorescein diacetate (DCFH-DA). which is de-esterified and subsequently oxidized by oxygen radicals to a fluorescent product 2', 7' -dichlorofluorescein ( DCF). There was a significant agedependent decrease in the formation rate of oxygen radicals. observed by decreased formation of DCF. No difference in oxygen radical formation was apparent between age groups following an in vitro challenge with an ascorbate/FeSO. mixture. This age-dependent decrease in cerebral oxygen radical generation coincided with age-dependent increases in superoxide dismutase. No age-related alterations in lipid order in either the hydrophilic or lipophilic membrane regions were observed using fluorescence polarization analysis. Age-dependent losses in cerebral P2 tryptophan fluorescence (a measure of protein degradation). and increased liberation of [ 1 •C)protein fragments into the acid-soluble fraction (a measure of overall proteolytic activity) were observed. Results suggest that aging does not proceed as a result of elevated rates of generation of oxygen radicals, a finding that does not support the proposed free radical theory of aging. The observed age-dependent decrease in the formation of oxygen radicals does not effect membrane lipid order. These findings implicate modifications in proteins and activated protein catabolic pathways as major contributing factors in the normal physiological process of senescence.

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“…Conflicting reports have been published on agerelated changes in rat brain for peroxidative potential (Sawada and Carlson, 1987;Devasagayam, 1989;Cand and Verdetti, 1989;LeBel and Bondy, 199lb), activities of superoxide dismutase, catalase, glutathione peroxidase and cytochrome oxidase (Vitorica et al, 1984;Scarpa et al, 1987;Cand and Verdetti, 1989;Vanella et al, 1989;Barja de Quiroga et al, 1990;Semsei et al, 1991;LeBel and Bondy, 199lb), in the glutathione system (Benzi et al, 1989;Devasagayam, 1989;Barja de Quiroga et al, 1990) and in neuronal calcium turnover (Gibson and Peterson, 1987). Membrane rigidity has also been an area of controversy in aging research.…”

Section: Age-related Alterations In the Brain Associated With Reactivmentioning

confidence: 99%