Superoxide dismutase and catalase significantly improve the osteogenic differentiation potential of osteogenetically compromised human adipose tissue-derived stromal cells in vitro

Sahlender, Benita; Windolf, Joachim; Suschek, Christoph V.

doi:10.1016/j.scr.2022.102708

Cited by 9 publications

(8 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Mammalian cells have many anti-oxidative defense components to secure against oxidative stress. This incorporates the expression of specialized proteins such as catalase, superoxide dismutase, and a few peroxidases, as well as unspecific ROS-degrading antioxidants such as vitamin E, ascorbate, uric corrosive, carotenoids, or glutathione [ 93 ]. Therefore, using antioxidant supplements could increase the expression of exogenous antioxidant proteins and enzymes to improve the antioxidant ability of BM-MSCs, but their effect is limited [ 94 ].…”

Section: Discussionmentioning

confidence: 99%

Sodium selenite preserves rBM-MSCs’ stemness, differentiation potential, and immunophenotype and protects them against oxidative stress via activation of the Nrf2 signaling pathway

Rahimi

Panahi

Lotfi

et al. 2023

BMC Complement Med Ther

View full text Add to dashboard Cite

Background The physiological level of reactive oxygen species (ROS) is necessary for many cellular functions. However, during the in-vitro manipulations, cells face a high level of ROS, leading to reduced cell quality. Preventing this abnormal ROS level is a challenging task. Hence, here we evaluated the effect of sodium selenite supplementation on the antioxidant potential, stemness capacity, and differentiation of rat-derived Bone Marrow MSCs (rBM-MSCs) and planned to check our hypothesis on the molecular pathways and networks linked to sodium selenite’s antioxidant properties. Methods MTT assay was used to assess the rBM-MSCs cells’ viability following sodium selenite supplementation (concentrations of: 0.001, 0.01, 0.1, 1, 10 µM). The expression level of OCT-4, NANOG, and SIRT1 was explored using qPCR. The adipocyte differentiation capacity of MSCs was checked after Sodium Selenite treatment. The DCFH-DA assay was used to determine intracellular ROS levels. Sodium selenite-related expression of HIF-1α, GPX, SOD, TrxR, p-AKT, Nrf2, and p38 markers was determined using western blot. Significant findings were investigated by the String tool to picture the probable molecular network. Results Media supplemented with 0.1 µM sodium selenite helped to preserve rBM-MSCs multipotency and keep their surface markers presentation; this also reduced the ROS level and improved the rBM-MSCs’ antioxidant and stemness capacity. We observed enhanced viability and reduced senescence for rBM-MSCs. Moreover, sodium selenite helped in rBM-MSCs cytoprotection by regulating the expression of HIF-1 of AKT, Nrf2, SOD, GPX, and TrxR markers. Conclusions We showed that sodium selenite could help protect MSCs during in-vitro manipulations, probably via the Nrf2 pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

Sodium selenite preserves rBM-MSCs’ stemness, differentiation potential, and immunophenotype and protects them against oxidative stress via activation of the Nrf2 signaling pathway

Rahimi

Panahi

Lotfi

et al. 2023

BMC Complement Med Ther

View full text Add to dashboard Cite

show abstract

“…Osteogenic differentiation of ASCs and DFATs was induced by DMEM containing 4.5 g/L glucose, 10% FCS, 1% Penicillin/Streptomycin, 100 nM dexamethasone, 50 µM L-ascorbic-2-phosphate and 10 mM β-glycerophosphate [ 32 ] and the degree of mineralization of the extracellular matrix as main parameter of osteogenic differentiated was quantified by Alizarin Red S staining [ 33 ] exactly as described previously [ 34 , 35 ].…”

Section: Methodsmentioning

confidence: 99%

Comparative Study of the Osteogenic Differentiation Potential of Adipose Tissue-Derived Stromal Cells and Dedifferentiated Adipose Cells of the Same Tissue Origin under Pro and Antioxidant Conditions

et al. 2022

Self Cite

View full text Add to dashboard Cite

Adipose tissue-derived stromal cells (ASCs) play an important role in various therapeutic approaches to bone regeneration. However, such applications become challenging when the obtained cells show a functional disorder, e.g., an impaired osteogenic differentiation potential (ODP). In addition to ASCs, human adipose tissue is also a source for another cell type with therapeutic potential, the dedifferentiated fat cells (DFATs), which can be obtained from mature adipocytes. Here, we for the first time compared the ODPs of each donors ASC and DFAT obtained from the same adipose tissue sample as well as the role of oxidative stress or antioxidative catalase on their osteogenic outcome. Osteogenic potential of ASC and DFAT from nine human donors were compared in vitro. Flow cytometry, staining for calcium accumulation with alizarin red, alkaline phosphatase assay and Western blots were used over an osteogenic induction period of up to 14 days. H2O2 was used to induce oxidative stress and catalase was used as an antioxidative measure. We have found that ASC and DFAT cultures’ ODPs are nearly identical. If ASCs from an adipose tissue sample showed good or bad ODP, so did the corresponding DFAT cultures. The inter-individual variability of the donor ODPs was immense with a maximum factor of about 20 and correlated neither with the age nor the sex of the donors of the adipose tissue. Oxidative stress in the form of exogenously added H2O2 led to a significant ODP decrease in both cell types, with this ODP decrease being significantly lower in DFAT cultures than in the corresponding ASC cultures. Regardless of the individual cell culture-specific ODP, however, exogenously applied catalase led to an approx. 2.5-fold increase in osteogenesis in the ASC and DFAT cultures. Catalase appears to be a potent pro-osteogenic factor, at least in vitro. A new finding that points to innovative strategies and therapeutic approaches in bone regeneration. Furthermore, our results show that DFATs behave similarly to ASCs of the same adipose tissue sample with respect to ODPs and could therefore be a very attractive and readily available source of multipotent stem cells in bone regenerative therapies.

show abstract

“…These limitations also affect the clinical applications of stem cells. Therefore, catalytic activities of antioxidant enzymes have been studied for ameliorating ROS for stem cell‐based osteogenic differentiation, tissue regeneration, etc [19–22] . However, the reduced stability of enzymes and their sensitivity to in vivo conditions hinder their practical use.…”

Section: Figurementioning

confidence: 99%

“…Therefore, catalytic activities of antioxidant enzymes have been studied for ameliorating ROS for stem cell-based osteogenic differentiation, tissue regeneration, etc. [19][20][21][22] However, the reduced stability of enzymes and their sensitivity to in vivo conditions hinder their practical use. To address this challenge, the development of antioxidant enzyme-based artificial enzymes that can efficiently scavenge ROS is necessary.…”

mentioning

confidence: 99%

Coordinated Axial Ligand and d‐π Conjugated Network Makes the Difference: Engineered 2D Mn‐Based Antioxidase Mimic for Enhancing Stem Cell Protection

Fatrekar,

Sreeram,

Vernekar

2023

ChemMedChem

View full text Add to dashboard Cite

Reactive oxygen species (ROS) refer to various partially reduced oxygen moieties that are naturally generated due to biochemical processes. Elevated formation of ROS leads to damage to biomolecules, resulting in oxidative stress and cell death. The increased level of ROS also affects therapeutics based on stem cell transplantation. Nanomaterials‐based enzyme mimetics have attracted immense attention, but there are several challenges to be addressed in terms of selectivity, efficiency, and biocompatibility. This highlight focuses on a recent investigation by Cheng and coworkers, who engineered an Mn‐superoxide dismutase (Mn‐SOD)‐inspired material with Mn‐N5 sites having an axial ligand and 2D d‐π‐conjugated network. This engineering approach enhances antioxidase‐like function and effectively rescues stem cells from ROS. In addition, it also protects osteogenesis‐related gene transcription, ensuring survival rates and osteogenic differentiation of hMSCs under ROS environment. This versatile and robust artificial antioxidase holds promise for stem cell therapies and ROS‐originated diseases.

show abstract

Superoxide dismutase and catalase significantly improve the osteogenic differentiation potential of osteogenetically compromised human adipose tissue-derived stromal cells in vitro

Cited by 9 publications

References 50 publications

Sodium selenite preserves rBM-MSCs’ stemness, differentiation potential, and immunophenotype and protects them against oxidative stress via activation of the Nrf2 signaling pathway

Sodium selenite preserves rBM-MSCs’ stemness, differentiation potential, and immunophenotype and protects them against oxidative stress via activation of the Nrf2 signaling pathway

Comparative Study of the Osteogenic Differentiation Potential of Adipose Tissue-Derived Stromal Cells and Dedifferentiated Adipose Cells of the Same Tissue Origin under Pro and Antioxidant Conditions

Coordinated Axial Ligand and d‐π Conjugated Network Makes the Difference: Engineered 2D Mn‐Based Antioxidase Mimic for Enhancing Stem Cell Protection

Contact Info

Product

Resources

About