Superoxide dismutase activity of Mycobacterium tuberculosis isolated from tuberculosis patients and the immunoreactivity of superoxide dismutase from M. tuberculosis H37Rv

Deshpande, Rajashri G.; Khan, Mahfuz; Bhat, Deepashree A.; Navalkar, R. G.

doi:10.1016/0962-8479(93)90082-9

Cited by 10 publications

(11 citation statements)

References 17 publications

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“…A blast search of protein databases showed that M.tuberculosis SodA homologs are present in M.avium complex and other environmental mycobacteria. However, there is enough experimental evidence to support that several of SodA epitopes (the most immunogenic B-cell epitope is with peptide sequence 7–26) are recognized in PPD positive individuals as well as in tuberculosis (TB) and leprosy patients404142. Moreover, previously in our laboratory, we have shown the potential of DNAsod in imparting protection against M.tuberculosis challenge in guinea pigs22.…”

Section: Discussionmentioning

confidence: 99%

Adjunctive immunotherapy with α-crystallin based DNA vaccination reduces Tuberculosis chemotherapy period in chronically infected mice

Chauhan

Jain

Dey

et al. 2013

Sci Rep

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By employing modified Cornell model, we have evaluated the potential of adjunctive immunotherapy with DNA vaccines to shorten the tuberculosis chemotherapy period and reduce disease reactivation. We demonstrate that α-crystallin based DNA vaccine (DNAacr) significantly reduced the chemotherapy period from 12 weeks to 8 weeks when compared with the chemotherapy alone. Immunotherapy with SodA based DNA vaccine (DNAsod) reduced the pulmonary bacilli only as much as DNAvec. Both DNAacr and DNAsod, although significantly delayed the reactivation in comparison to the chemotherapy alone, this delay was associated with the immunostimulatory sequences present in the vector backbone and was not antigen specific. Both DNA vaccines resulted in the production of significantly higher number of TEM cells than the chemotherapy alone, however, only in the case of DNAsod, this enhancement was significant over the DNAvec treatment. Overall, our findings emphasize the immunotherapeutic potential of DNAacr in shortening the duration of TB chemotherapy.

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Section: Discussionmentioning

confidence: 99%

Adjunctive immunotherapy with α-crystallin based DNA vaccination reduces Tuberculosis chemotherapy period in chronically infected mice

Chauhan

Jain

Dey

et al. 2013

Sci Rep

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“…SOD is one of the largest secretory proteins among the bacterial culture filtrate proteins in growing M. tuberculosis cultures (5-7), and clinical M. tuberculosis isolates from tuberculosis patients are rich in SOD (8). These data indicate that SOD secretion may play a valuable role in M. tuberculosis pathogenesis.…”

Section: Introductionmentioning

confidence: 85%

The Role of Superoxide Dismutase in the Survival of <i>Mycobacterium tuberculosis</i> in Macrophages

2013

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SUMMARY:There is a large amount of information available regarding the chemical structure and biological activity of superoxide dismutase (SOD), which is abundantly generated by Mycobacterium tuberculosis in the early stages of growth. SOD is a strong superoxide radical scavenger, which plays a significant role in resisting oxidative stress. On the other hand, SOD mutant strains have been constructed to define the role of this molecule in the immune response to M. tuberculosis infection. These studies have suggested that the absence or attenuation of SOD can motivate innate immunity and SOD avoid destruction or growth inhibition of M. tuberculosis. For detailed investigation of how SOD proteins aid M. tuberculosis survival within macrophages, we cloned 2 SOD genes (SODA and SODC) from the M. tuberculosis H37Rv genome, overexpressed, identified, and purified the proteins, and then exposed macrophages to the proteins. Following this, we assessed NO production, the secretion of cytokine interferon-g (IFN-g) and intercellular adhesion molecule-1, the expression of IFN-g receptor and Toll-like receptor 2 on the surface of macrophages, and caspase-3 enzyme activity as well as macrophage apoptosis. Our results showed that both SODA and SODC proteins considerably reduced the production of NO and oxygen radicals and impaired cell immunologic function in early infection.

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“…Moreover, macrophages-derived NO could also decrease host susceptibility to M.tb and the virulence of M.tb [24]. In addition to NO, superoxide dismutase (SOD) and superoxide dismutase C (SodC) in activated macrophages also demonstrate resistance activities against M.tb infection [25,26].…”

Section: Introductionmentioning

confidence: 99%

The fibroblast growth factor-2 arrests Mycobacterium avium sp. paratuberculosis growth and immunomodulates host response in macrophages

Wang

Yao

et al. 2015

Tuberculosis

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Superoxide dismutase activity of Mycobacterium tuberculosis isolated from tuberculosis patients and the immunoreactivity of superoxide dismutase from M. tuberculosis H37Rv

Cited by 10 publications

References 17 publications

Adjunctive immunotherapy with α-crystallin based DNA vaccination reduces Tuberculosis chemotherapy period in chronically infected mice

Adjunctive immunotherapy with α-crystallin based DNA vaccination reduces Tuberculosis chemotherapy period in chronically infected mice

The Role of Superoxide Dismutase in the Survival of <i>Mycobacterium tuberculosis</i> in Macrophages

The fibroblast growth factor-2 arrests Mycobacterium avium sp. paratuberculosis growth and immunomodulates host response in macrophages

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