Superoxide Dismutase Activity Measurement Using Cytochromec-Modified Electrode

Abstract

SOD activity was quantified by the use of a cytochrome c-modified gold electrode. The electrode responded rapidly to superoxide radicals in solution. Steady-state superoxide concentrations were established by control of the calibration conditions. On this basis very low SOD activities were detected (10-200 munits/mL). This method showed good correlation with the standard photometric test and was applied for the determination of SOD activity entrapped into liposomes. Interference by hydrogen peroxide and uric acid was characterized and minimized using long-chain thiols for the first electrode modification step. The complete modification proved to be stable for several days.

A Reactive Oxygen/ZNitrogen Species Sensor Fabricated from an Electrode Modified with a Polymerized Iron Porphyrin and a Polymer Electrolyte Membrane

“…[8][9][10] However, these methods are not suitable for in vivo and real-time O2 -• determination. 11 Conversely, several electrochemical methods using electrodes modified with superoxide dismutase (SOD) and cytochrome c have been shown to be useful for real-time detection. [12][13][14] For NO detection, the chemiluminescence method, the Griess method, and ESR methods are conventionally employed. )…”

Determination of Copper(II) by Anodic Stripping Voltammetry at a 2,5-Dimercapto-1,3,4-thiadiazol Self-assembled Monolayer-based Gold Electrode

“…9 SAM electrodes are very promising for the construction of electrochemical sensors because they can enhance the selectivity and sensitivity, improve the response time, and decrease the overpotential of the redox process of analytes, while exhibiting good stability. [10][11][12] Based on such prominent properties, the application of SAMs to the estimation of copper has been investigated. Rubinstein reported that a mixed functionalized SAM recognized selectively Cu(II) ions in the presence of other ions.…”

Amperometric Detection of Superoxide Dismutase at Cytochrome c-Immobilized Electrodes: Xanthine Oxidase and Ascorbate Oxidase Incorporated Biopolymer Membrane for in-vivo Analysis

“…[1][2][3][4][5][6] Detection of superoxide and SOD has been intensively pursued by spectrophotometric and electrochemical methods. [7][8][9][10][11][12][13][14][15][16] Electrochemical sensors are of great interest due to their advantages, which include microfabrication and in-vivo and in-vitro applications. 17,18 The hemoprotein, cytochrome c (cyt c), undergoes facile reductive reaction with superoxide.…”

“…Electrochemical biosensors for superoxide with cyt c as the sensing element were constructed employing a suitable electron-transfer promoter. [11][12][13][14] SOD is a selective scavenger of superoxide, and the best way for the detection of SOD thus could be the estimation of superoxide. Superoxide undergoes spontaneous disproportionation to produce H2O2, an electroactive species.…”