Superoxide Dismutase Activity in Renal Cell Carcinoma

Nakada, Teruhiro; Akiya, Tohru; Koike, Hiroshi; Katayama, Takashi

doi:10.1159/000472897

Cited by 14 publications

(7 citation statements)

References 14 publications

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“…Many studies supporting the conclusions that human cancer tissue is low in MnSOD activities have been reviewed by Oberley and Oberley [20]. MnSOD activities in RCC tissues have been reported to be lower [21], equal [22,23], or higher [21] than those in corresponding normal tissues. However, there are few reports on the alterations of MnSOD at the protein level in RCC.…”

Section: Discussionmentioning

confidence: 99%

Differential protein profiling in renal‐cell carcinoma

Shi

Fan

Liou

et al. 2004

Molecular Carcinogenesis

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Characterizing the alterations of protein expression in cancer cells can be very useful in providing insight into the changes in the functional pathways and thus the fundamental mechanisms of cancer development at the molecular level. In this study, we profiled protein expressions in eleven pairs of primary cell cultures derived from renal-cell carcinoma (RCC) tissues and patient-matched normal kidney tissues utilizing two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Together with the immunoblot analysis of proteins from the RCC tissues, the study also demonstrated that the alterations of protein expression observed in RCC primary cell cultures reflected those observed in the original RCC tissues. We analyzed the expression profiles and identified proteins differentially expressed in RCC primary cell cultures by 2-D PAGE and mass spectrometry (MS). We found sixteen proteins were overexpressed and seven proteins underexpressed in RCC. The deregulated expressions of proteins include those involved in metabolism, cellular morphology, heat shock response, cell growth, etc. Overexpression of three proteins, alphabeta-crystallin, manganese superoxide dismutase (MnSOD), and annexin IV, most commonly observed in primary RCC cell cultures, were also observed by immunoblot analysis of proteins from the RCC tissues from which the primary cell cultures were derived. Semi-quantitative reverse transcription (RT)-polymerase chain reaction (PCR) analysis revealed the direct correlation between deregulated gene expression and the corresponding protein abundance in two of the three most commonly upregulated proteins we found in RCC.

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Section: Discussionmentioning

confidence: 99%

Differential protein profiling in renal‐cell carcinoma

Shi

Fan

Liou

et al. 2004

Molecular Carcinogenesis

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“…Nakada et al [19] found no changes in SOD activity in renal carcinoma. They reported that the age of the patients and the grade or stage of the tumors had no apparent effect on SOD activity.…”

Section: Discussionmentioning

confidence: 93%

Superoxide dismutase and glutathione peroxidase enzyme activities in larynx carcinoma

Kalaycı

Öztürk

Öztürk³

et al. 2005

Acta Oto-Laryngologica

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“…For instance, in a study the activities of SOD and GSH-Px enzymes were found lower in malignant liver tissues during rat hepatocarcinogenesis (26). In another one, Corrocher et al (27) established that in human hepatoma the enzymatic antioxidant system was severely impaired owing to lowered GSH-Px activities. Nakada et al (28) measured SOD activities in renal cell carcinoma and nontumorous renal tissues.…”

Section: Resultsmentioning

confidence: 99%