Superoxide dismutase: a review and a modified protocol for activities measurements in rat livers

Campos-Shimada, Lilian Brites; Gilglioni, Eduardo Hideo; Garcia, Renata Porto Alegre; Martins-Maciel, Elismari Rizato; Ishii–Iwamoto, Emy Luiza; Salgueiro-Pagadigorria, Clairce Luzia

doi:10.1080/13813455.2018.1520891

Cited by 19 publications

(10 citation statements)

References 68 publications

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“…Binding of Ang II to the Ang II type 1 receptor (AT 1 R) enhances the activation of NOX ( Masi et al, 2019 ), which is a substantial producer of ROS, including O 2 −• ( Wen et al, 2019 ). The elevated levels of O 2 −• detected in the H9c2 cells depleted the antioxidant SOD, which functions as a first line of defense against cardiomyocyte hypertrophy by converting the radical anion into water and hydrogen peroxide ( Campos-Shimada et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Parkia speciosa Hassk. Empty Pod Extract Alleviates Angiotensin II-Induced Cardiomyocyte Hypertrophy in H9c2 Cells by Modulating the Ang II/ROS/NO Axis and MAPK Pathway

et al. 2021

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Cardiac hypertrophy is characteristic of heart failure in patients who have experienced cardiac remodeling. Many medicinal plants, including Parkia speciosa Hassk., have documented cardioprotective effects against such pathologies. This study investigated the activity of P. speciosa empty pod extract against cardiomyocyte hypertrophy in H9c2 cardiomyocytes exposed to angiotensin II (Ang II). In particular, its role in modulating the Ang II/reactive oxygen species/nitric oxide (Ang II/ROS/NO) axis and mitogen-activated protein kinase (MAPK) pathway was examined. Treatment with the extract (12.5, 25, and 50 μg/ml) prevented Ang II-induced increases in cell size, NADPH oxidase activity, B-type natriuretic peptide levels, and reactive oxygen species and reductions in superoxide dismutase activity. These were comparable to the effects of the valsartan positive control. However, the extract did not significantly ameliorate the effects of Ang II on inducible nitric oxide synthase activity and nitric oxide levels, while valsartan did confer such protection. Although the extract decreased the levels of phosphorylated extracellular signal-related kinase, p38, and c-Jun N-terminal kinase, valsartan only decreased phosphorylated c-Jun N-terminal kinase expression. Phytochemical screening identified the flavonoids rutin (1) and quercetin (2) in the extract. These findings suggest that P. speciosa empty pod extract protects against Ang II-induced cardiomyocyte hypertrophy, possibly by modulating the Ang II/ROS/NO axis and MAPK signaling pathway via a mechanism distinct from valsartan.

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Section: Discussionmentioning

confidence: 99%

Parkia speciosa Hassk. Empty Pod Extract Alleviates Angiotensin II-Induced Cardiomyocyte Hypertrophy in H9c2 Cells by Modulating the Ang II/ROS/NO Axis and MAPK Pathway

et al. 2021

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“…The specific proteins that experienced highly significant changes as a result of cryopreservation were also evaluated and thus can be discriminants for the major consequences of stress imposed by the procedure. Cryopreservation caused a marked and highly significant decrease in the amount of Superoxide dismutase (Cu–Zn) (SOD1), an enzyme which forms part of the first line of defense against oxidative stress in most organisms. − Although it has previously been reported that this enzyme is affected by cryopreservation, , this is the first time that SOD1 is identified as a discriminating variable using bioinformatic analysis, being one of the most highly significantly different proteins between fresh and frozen thawed semen. This finding strongly supports the theory that alterations in redox regulation and oxidative stress are a major factor involved in cryodamage as also seen in the present study, indicated by the increased level of 4-HNE in cryopreserved spermatozoa.…”

Section: Discussionmentioning

confidence: 98%

In Stallion Spermatozoa, Superoxide Dismutase (Cu–Zn) (SOD1) and the Aldo-Keto-Reductase Family 1 Member b (AKR1B1) Are the Proteins Most Significantly Reduced by Cryopreservation

et al. 2021

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Although cryopreservation is widely used in animal breeding, the technique is still suboptimal. The population of spermatozoa surviving the procedure experiences changes attributed to alteration in their redox regulation. In order to expand our knowledge regarding this particular aspect, the proteome in fresh and frozen thawed aliquots of equine spermatozoa was studied to identify the proteins most severely affected by the procedure. If alteration of redox regulation is a major factor explaining cryodamage, proteins participating in redox regulation should be principally affected. Using a split sample design, 30 ejaculates from 10 different stallions were analyzed as fresh spermatozoa, and another aliquot from the same ejaculate was analyzed as a frozen thawed sample. The proteome was studied under both conditions using UHPLC-MS/MS and bioinformatic analysis conducted to identify discriminant variables between both conditions. Data are available through the ProteomeXchange Consortium with identifier PXD022236. The proteins most significantly reduced were Aldo-keto reductase family 1 member B ( p = 2.2 × 10 –17 ) and Superoxide dismutase (Cu–Zn) ( p = 4.7 × 10 –14 ). This is the first time that SOD1 has been identified as a discriminating variable using bioinformatic analysis, where it was one of the most highly significantly different proteins seen between fresh and frozen thawed semen. This finding strongly supports the theory that alteration in redox regulation and oxidative stress is a major factor involved in cryodamage and suggests that control of redox regulation should be a major target to improve current cryopreservation procedures.

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“…Catalase (CAT) activity was evaluated according to the method described by Chance and Greenstein 1992 27 . Meanwhile, the activities of superoxide dismutase (SOD) 28 and glutathione peroxidase (GPx) were determined using the method of Misra and Fridovich (1972) 29 .…”

Section: Antioxidants and Oxidative Stressmentioning

confidence: 99%

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2021

IJPSR

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The study was aimed at knowing the effect of mobile phone electromagnetic radiation (EMR) on fertility in Wistar Albino Rats and to assess the capability of polyphenolic rich Withania somnifera root extract in shielding rat testis against EMR-induced injuries. Methodology: One hundred and forty-four male Wistar Albino Rats were included in the study and grouped in to 4. Group I received a standard diet, Group II exposed to active mobile phone radiation of 2700 MHz fields for 3 hours/day, Group III exposed to 2700 MHz fields for 3 hours/day along with administration of 250 mg/kg aqueous extract of Withania somnifera root (Aq-Wsr) orally and Group IV received standard diet and 250 mg/kg Aq-Wsr during the experimental period of 180 days. Results: Group-II showed a significant decline in the sperm parameters and reduced in-vivo antioxidants. Group III revealed significant augmentation of sperm parameters and regulated the in vivo antioxidants. On microscopic examination, Group II showed irregular seminiferous tubules, atrophy of spermatogonial cells, hyper-cellularity, degenerated spermatozoa, and decreased number of Leydig cells. Conclusion: This research concludes that long-term exposure to EMR results in testicular trauma, which was statistically significant in sperm parameters and microscopic changes of testis in Group II. This study also proved the shielding effect of Withania somnifera root extract against EMR.

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Superoxide dismutase: a review and a modified protocol for activities measurements in rat livers

Cited by 19 publications

References 68 publications

Parkia speciosa Hassk. Empty Pod Extract Alleviates Angiotensin II-Induced Cardiomyocyte Hypertrophy in H9c2 Cells by Modulating the Ang II/ROS/NO Axis and MAPK Pathway

Parkia speciosa Hassk. Empty Pod Extract Alleviates Angiotensin II-Induced Cardiomyocyte Hypertrophy in H9c2 Cells by Modulating the Ang II/ROS/NO Axis and MAPK Pathway

In Stallion Spermatozoa, Superoxide Dismutase (Cu–Zn) (SOD1) and the Aldo-Keto-Reductase Family 1 Member b (AKR1B1) Are the Proteins Most Significantly Reduced by Cryopreservation

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