Superoxide Anions Induce the Maturation of Human Dendritic Cells

Kantengwa, Salomé; Jornot, Lan; Devenoges, Christiane; Nicod, Laurent P.

doi:10.1164/rccm.200205-425oc

Cited by 118 publications

(80 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…5). These results indicate that ROS, as demonstrated in the activation and maturation processes [22][23][24], also play a crucial role in DC differentiation and that mitochondria are an important source of ROS in the process.…”

Section: Discussionmentioning

confidence: 72%

See 1 more Smart Citation

Role of mitochondria and reactive oxygen species in dendritic cell differentiation and functions

Prete

Zaccagnino

Paola

et al. 2008

Free Radical Biology and Medicine

100

View full text Add to dashboard Cite

Dendritic cells (DC) are potent antigen-presenting cells capable of inducing T and B responses and immune tolerance. We have characterized some aspects of energy metabolism accompanying the differentiation process of human monocytes into DC. Compared to precursor monocytes, DC exhibited a much larger number of mitochondria and consistently (i) a higher endogenous respiratory activity and (ii) a more than sixfold increase in ATP content and an even larger increase in the activity of the mitochondrial marker enzyme citrate synthase. The presence in the culture medium of rotenone, an inhibitor of the respiratory chain Complex I, prevented the increase in mitochondrial number and ATP level, without affecting cell viability. Rotenone inhibited DC differentiation, as revealed by the observation that the expression of CD1a, which is a specific surface marker of DC differentiation, was strongly reduced. Cells cultured in the presence of rotenone displayed a lower content of growth factor-induced, mitochondrially generated, hydrogen peroxide. A similar drop in ROS was observed upon addition of catalase, which caused functional effects similar to those produced by rotenone treatment. These results suggest that ROS play a crucial role in DC differentiation and that mitochondria are an important source of ROS in this process. © 2008 Elsevier Inc. All rights reserved.Keywords: Dendritic cells; Mitochondria; Reactive oxygen species; Oxidative phosphorylation; Free radicals Effective immune responses require correct localization and functioning of dendritic cells (DC). Dendritic cells are the most potent and versatile antigen-presenting cells, with a unique ability to induce specific immune responses as well as tolerance [1,2]. In peripheral tissues they reside in an immature state waiting for incoming antigens. After capturing and processing the antigens, DC undergo a maturation process which culminates in dramatic changes in functions and migratory properties [3,4]. The localization of mature DC to the draining lymph nodes coincides with the presentation of processed antigens to naïve T cells, triggering the initiation of specific immune responses [2,5]. An in vitro method to differentiate immature DC from CD14 + monocyte precursors cultured in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) is very well established [6,7].Reactive oxygen species (ROS) have been identified as important second messengers involved in the transduction of several signaling pathways [8,9], gene expression, and cell proliferation [10]. Furthermore, recent studies have shown that growth factors, through the actions of their specific receptors, are able to increase

show abstract

Section: Discussionmentioning

confidence: 72%

“…Recent studies showed that, whereas NADPH oxidase activation is required for DC killing of intracellular Escherichia coli, no role in DC differentiation or functions can be ascribed to NADPH oxidasederived oxygen radicals [20,21]. However, an involvement of ROS has been recently described in the activation and maturation process of DC [22][23][24].…”

mentioning

confidence: 99%

Role of mitochondria and reactive oxygen species in dendritic cell differentiation and functions

Prete

Zaccagnino

Paola

et al. 2008

Free Radical Biology and Medicine

100

View full text Add to dashboard Cite

show abstract

“…In DC, oxygen radicals were implicated in both DC differentiation (43) and maturation (26,27) and in IL-8/CXCL8 production (24,25). Controversial data are present in the literature about the inhibitory action (52) or the positive role (26,53) of oxygen radicals on the ability of DC to stimulate T cell response.…”

Section: Discussionmentioning

confidence: 99%

Toll Receptor-Mediated Regulation of NADPH Oxidase in Human Dendritic Cells

Vulcano

Dusi

Lissandrini

et al. 2004

The Journal of Immunology

122

108

View full text Add to dashboard Cite

Activation of NADPH oxidase represents an essential mechanism of defense against pathogens. Dendritic cells (DC) are phagocytic cells specialized in Ag presentation rather than in bacteria killing. Human monocyte-derived DC were found to express the NADPH oxidase components and to release superoxide anions in response to phorbol esters and phagocytic agonists. The NADPH oxidase components p47phox and gp91phox were down-regulated during monocyte differentiation to DC, and maturation of DC with pathogen-derived molecules, known to activate TLRs, increased p47phox and gp91phox expression and enhanced superoxide anions release. Similar results were obtained with plasmacytoid DC following maturation with influenza virus. In contrast, activation of DC by immune stimuli (CD40 ligand) did not regulate NADPH oxidase components or respiratory burst. NADPH oxidase-derived oxygen radicals did not play any role in DC differentiation, maturation, cytokine production, and induction of T cell proliferation, as based on the normal function of DC generated from chronic granulomatous disease patients and the use of an oxygen radical scavenger. However, NADPH oxidase activation was required for DC killing of intracellular Escherichia coli. It is likely that the selective regulation of oxygen radicals production by pathogen-activated DC may function to limit pathogen dissemination during DC trafficking to secondary lymphoid tissues.

show abstract

“…Also, tobacco smoke has many toxic low molecular weight compounds that are able to directly stimulate bronchoalveolar dendritic cells (DCs). Activated DCs can discharge many of inflammatory chemokines that will induce the recruitment and activation of more DCs and other inflammatory cells such as neutrophils 13 .The pathogenic changes of lung consequenced due to its immune responsive on cigarette smoke and similar observation was found by other worker [13][14] . This observation may suggest effects of cigarettes smoke on the lung could be due to metabolites of cigarette smoke in the lung that can change antioxidant defense system leading to oxidative stress.…”

Section: Discussionmentioning

confidence: 61%

Ameliorative Effect of Tocopheryl Acetate in Lung of Cigarette Smoke Exposed Adult Swiss Albino Mice J

Chaudhary¹,

Singh²,

Shamal³

et al. 2017

IJTA

View full text Add to dashboard Cite

Lung is the one of the first organ to metabolise the cigarette smoke contents soon after inhalation. Adult male mice were assigned to six different groups different groups (Group I (control),Group II (Tocopheryl acetate), Group III (soyabean oil used as vehicle for Tocopheryl acetate), Group IV (Cigarette smoke Exposed), Group V (Cigarette smoke exposed plus Tocopheryl acetate) and Group VI(Cigarette smoke exposed plus soyabean oil) were exposed to cigarette smoke 3 times a day for 20 minutes each time for 28 days and Tocopheryl acetate with dose of 200mg/kg/day in 0.3ml of soyabean oil as vehicle orally through oral gavage. Cigarette smoke exposed lung appreciated highly in pathogenicby lymphatic nodular around hyperplasia bronchus collapsed alveolar sac, inflammatory interalveolar septum. Lung of cigarette smoke exposed group showed significantly increased oxidant level as malondialdehyde 815.2±54.62 µmol/mg protein (Group V) and 823.5±68.17 µmol/mg protein (Group VI).Tocopheryl acetate administrated to cigarette smoke exposed lung(Group V) markedly reduced the severity of the pathogenesis and oxidant level(570.5±56.32 µmol/mg protein). Present study, tocopheryl acetate thought to be as a protective agent against cigarette smoke.

show abstract

Superoxide Anions Induce the Maturation of Human Dendritic Cells

Cited by 118 publications

References 31 publications

Role of mitochondria and reactive oxygen species in dendritic cell differentiation and functions

Role of mitochondria and reactive oxygen species in dendritic cell differentiation and functions

Toll Receptor-Mediated Regulation of NADPH Oxidase in Human Dendritic Cells

Ameliorative Effect of Tocopheryl Acetate in Lung of Cigarette Smoke Exposed Adult Swiss Albino Mice J

Contact Info

Product

Resources

About