Superoxide anion scavenging effect and superoxide dismutase activity ofGinkgo biloba extract

Abstract

Ginkgo biloba extract is known to be efficient in diseases associated with free radical generation. The purpose of this work was to study, under in vitro conditions, the action of Ginkgo biloba extract (Gbe) against superoxide anion (O2-.), which is directly or indirectly implicated in cell damage. Gbe appears to have both an O2-. scavenging effect and also a superoxide dismutase activity. Its antiradical effect was demonstrated by low temperature electron spin resonance and in a non-enzymatic system (phenazine methosulfate-NADH), and its enzymatic activity was shown by polarographic determination.

EGb 761 protects liver mitochondria against injury induced by in vitro anoxia/reoxygenation

“…In the present study, we decided to use EGb 761, the Ginkgo biloba extract. EGb 761 was well known for its antioxidant properties [3,12,14,15] and its protective effects against injuries induced by in vivo ischemia/reperfusion [2,7,8] EGb 761 was used at 50, 100, and 200 g/ml, concentrations that did not impair the mitochondrial respiratory parameters before anoxia/reoxygenation (Figs. 3A and 4).…”

“…Recent studies have provided considerable support for the in vitro and in vivo protective effects of EGb 761 in ischemia/reperfusion injury [5][6][7][8] or oxidative stress [9 -11]. These effects were closely related to the ability of EGb 761 to scavenge free radicals such as superoxide anion [12,13], hydroxyl and peroxyl radicals [3,14], and nitric oxide [15]. Thus EGb 761 has wide antioxidant effects and inhibits peroxidation reactions in vivo and in vitro [16 -19].…”

“…EGb 761 protected the mitochondrial oxidative phosphorylation efficiency against damage induced by in vitro anoxia/reoxygenation, probably by its antioxidant action. EGb 761 was able to react with superoxide anion [12] and to scavenge free radicals. In our EPR experiments, we have shown that anoxia/reoxygenation in the presence of EGb 761 (200 g/ml) decreased the EPR signal intensity (Fig.…”

The Effect of Ginkgo Biloba Extract on Free Radical Production in Hypoxic Rats.

“…Many studies have targeted a superxide anion (O 2 Ϫ ) scavenging effect of Gb extract. 10) Recent studies of mitochondria from brain, liver or heart cells exposed to hypoxia and/or ischemic stress show the protective effect of Gb extract against oxidative stress and also a limiting effect membrane lipoperoxidation. [11][12][13][14][15] In our investigation, we aimed to determine the effect of prolonged Gb extract treatment on free radical production by phagocytic cells.…”

Suppression of Oxidized LDL-Induced PDGF Receptor .BETA. Activation by Ginkgo Biloba Extract Reduces MMP-1 Production in Coronary Smooth Muscle Cells

“…An extract of Ginkgo Biloba L., a mixture mainly composed of flavonoid glycosides (glycosides of quercetin and kaempferol) and terpenoides (ginkgolides and bilobalide), is shown to suppress the generation of reactive oxygen species (ROS) [18][19][20][21] as well as the oxidation of LDL 19,22) , which is a critical event in atherogenesis. The extract and its ingredients, especially ginkgolides, also exhibit an antagonistic effect on platelet-activating factor 23) .…”