Superoxide anion production in the interaction of wheat roots and rhizobacteria Azospirillum brasilense Sp245

Méndez-Gómez, Manuel; Castro-Mercado, Elda; Alexandre, Gladys; García-Pineda, Ernesto

doi:10.1007/s11104-015-2709-9

Cited by 5 publications

(1 citation statement)

References 59 publications

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“…The protein content was determined according to the Bradford method. The activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were measured using azalantetrazole reduction, guaiacol, and hydrogen peroxide, respectively [30,31]. The absorbance of each reaction was measured at wavelengths of 560 nm, 470 nm, and 240 nm, using a UV spectrophotometer (GENESY-50, Thermo Fisher Scientific, Waltham, MA, USA), respectively.…”

Section: Detection Of Reactive Oxygen Species (Ros) and Antioxidant E...mentioning

confidence: 99%

Two Nematicidal Compounds from Lysinimonas M4 against the Pine Wood Nematode, Bursaphelenchus xylophilus

Sun

Wang

et al. 2022

Forests

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A rich source of bioactive secondary metabolites from microorgannisms are widely used to control plant diseases in an eco-friendly way. To explore ideal candidates for prevention of pine wilt disease (PWD), a bacterial strain from rhizosphere of Pinus thunbergii, Lysinimonas M4, with nematicidal activity against pine wood nematode (PWN), Bursaphelenchus xylophilus, was isolated. Two nematicidal compounds were obtained from the culture of Lysinimonas M4 by silica gel chromatography based on bioactivity-guided fractionation and were subsequently identified as 2-coumaranone and cyclo-(Phe-Pro) by nuclear magnetic resonance (NMR) and mass spectrometry (MS). The 2-coumaranone and cyclo-(Phe-Pro) showed significant nematicidal activity against PWN, with LC50 values at 24 h of 0.196 mM and 0.425 mM, respectively. Both compounds had significant inhibitory effects on egg hatching, feeding, and reproduction. The study on nematicidal mechanisms revealed that 2-coumaranone and cyclo-(Phe-Pro) caused the accumulation of reactive oxygen species (ROS) in nematodes, along with a notable decrease in CAT and POS activity and an increase in SOD activity in nematodes, which might contribute to the death of pine wood nematodes. Bioassay tests demonstrated that the two compounds could reduce the incidence of wilting in Japanese black pine seedlings. This research offers a new bacterial strain and two metabolites for biocontrol against PWN.

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Section: Detection Of Reactive Oxygen Species (Ros) and Antioxidant E...mentioning

confidence: 99%