Superoxide Anion Production by the Spermatozoa of Men with Varicocele: Relationship with Varicocele Grade and Semen Parameters

Alkan, İlter; Yüksel, Meral; Canat, Lütfi; Atalay, Hasan Anıl; Can, Osman; Özveri, Hakan; Başar, Mehmet Murad

doi:10.5534/wjmh.180028

Cited by 21 publications

(19 citation statements)

References 26 publications

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“…These studies suggest that ADR has direct inhibitory effect on androgen biosynthesis in Leydig cells. The significant reduction in sperm count and motility might be due to increase in toxin ROS which is a crucial factor in sperm DNA fragmentation and sperm motility [24, 25]. This study also demonstrates that DA-9401 can improve sperm count, sperm motility, and serum testosterone level.…”

Section: Discussionmentioning

confidence: 64%

Cross-talk between ER stress and mitochondrial pathway mediated adriamycin-induced testicular toxicity and DA-9401 modulate adriamycin-induced apoptosis in Sprague–Dawley rats

et al. 2019

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Background DA-9401 was prepared as a mixture of Chinese medicinal herb extracts from roots of Morinda officinalis How (Rubiaceae), outer scales of Allium cepa L. (Liliceae) and seeds of Cuscuta chinensis Lamark (Convolvulaceae). The present study was designed to investigate the possible protective role of DA-9401 in adriamycin (ADR)-induced testicular toxicity associated with oxidative stress, endoplasmic reticulum (ER) stress, and apoptosis. Methods Fifty healthy 8-week-old male Sprague–Dawley rats were equally divided into five groups. The first CTR group was treated with normal saline 2 ml/day by gavage. The second was treated with DA-100 (DA-9401 100 mg/kg/day). The third (ADR) group received ADR (2 mg/kg/once a week) intraperitoneally, while the combination of ADR and DA-9401 was given to the fourth ADR + DA-100 (100 mg/kg/day p.o) group and fifth ADR + DA-200 (200 mg/kg/day p.o) group. At the end of the 8-week treatment period, body weight, reproductive organ weights, fertility rate, pups per female were recorded, and serum were assayed for hormone concentrations. Tissues were subjected to semen analysis, histopathological changes, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), oxidative stress markers and expression levels of endoplasmic reticulum (ER) stress markers, apoptosis markers, tight junction protein markers, steroidogenic acute regulatory protein (StAR), cation channel of sperm (CatSper) and glycogen synthase kinase-3 (GSK-3) by western blot. Results DA-9401 administration to ADR-treated rats significantly decreased serum luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels, interleukin-6, TNF-α, MDA level, ROS/RNS level, ER stress response protein levels, tunnel positive cells, cleaved caspase-3, and Bax/Bcl2 ratio. Moreover, pretreatment with DA-9401 significantly increased body weight, reproductive organ weights, fertility rate, pups per female, Johnsen’s score, spermatogenic cell density, sperm count and sperm motility, serum testosterone concentration, testicular superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), tight junction protein markers, star protein level, CatSper, and GSK-3 level. Conclusions ADR treatment can markedly impair testicular function and induce testicular cell death presumably by causing significant changes in oxidative stress, ER stress, and mitochondrial pathway. DA-9401 exerts beneficial effects against oxidative stress, ER stress, and mitochondria-mediated cell death pathway in testis tissue by up-regulating expression levels of tight junction protein markers, steroidogenic acute regulatory protein, GSK-3 alpha, and cation channels of sperm. Electronic supplementary material The online version of this article (10.1186/s12935-019-0805-2) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 64%

Cross-talk between ER stress and mitochondrial pathway mediated adriamycin-induced testicular toxicity and DA-9401 modulate adriamycin-induced apoptosis in Sprague–Dawley rats

et al. 2019

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“…Also, Mostafa, Anis, Nashar, Imam, and Osman () pointed to the significant increase of seminal plasma OS in cases associated with higher varicocele grades. Lately, Alkan et al () showed higher superoxide anion and total seminal ROS levels in varicocele grade III cases compared with grade II cases.…”

Section: Discussionmentioning

confidence: 99%

Seminal SIRT1‐oxidative stress relationship in infertile oligoasthenoteratozoospermic men with varicocele after its surgical repair

et al. 2019

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| INTRODUC TI ONVaricocele is among the causes of male factor infertility and is one of the most controversial issues concerning to whom varicocelectomy should be beneficial. Varicocele is found in about 40% of men complaining of primary infertility and 80% of men presented with secondary infertility, though it occurs in 12% of normzoospermic men (Brannigan, 2017;Hassanin, Ahmed, & Kaddah, 2018). Several theories were raised to explain how varicocele affects male infertility. It includes hypoxia, hyperthermia; impaired arterial perfusion, retrograde flow of adrenal/renal metabolites and increased seminal ROS Oxidative stress (OS) results from the disproportion between reactive oxygen species (ROS) and antioxidants. Lately, seminal OS gets impotence being responsible about sperm DNA damage, sperm membrane changes with decreased fertilising ability. Several studies showed high seminal OS generally in infertile men and particularly Abstract This work assessed seminal SIRT1-oxidative stress (OS) relationship in infertile oligoasthenoteratozoospermic (OAT) men after varicocele repair. Overall, thirty OAT men with varicocele were investigated. Inclusion criteria were infertile males (males who were unable to initiate a pregnancy within 1 year of regular unprotected intercourse), confirmed OAT and normal female factor. These cases were subjected to history taking, clinical checkup and semen analysis. In their semen, seminal SIRT1, malondialdehyde (MDA) and glutathione peroxidase (GPx) levels were assessed.These men were subjected to varicocele surgical repair and were followed up for 3 months. Post-operatively, the mean seminal SIRT1, GPx levels showed significant increases and the mean MDA level showed significant decrease compared to the pre-operative levels linked to improved sperm parameters. The mean seminal SIRT1, GPx, MDA levels showed more significant improvement in grade III varicocele cases compared to grade II cases after surgical repair. Seminal SIRT1 levels showed significant positive correlations with sperm concentration, sperm motility, sperm normal morphology, seminal GPx levels and a significant negative correlation with seminal MDA levels. It could be concluded that seminal SIRT1 is significantly decreased in infertile OAT men with varicocele after its surgical repair linked to improved sperm parameters as well as seminal OS. K E Y W O R D Smale infertility, oxidative stress, semen, SIRT1, varicocele

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“…Our work indicated that formalin declined sperm motility. As stated by Alkan and colleagues immotile and atypical sperm are able to generate superoxidase, which has an oxidizing effect can decline the sperm quality like motility (43).…”

Section: Discussionmentioning

confidence: 94%

L-Carnitine reduces the negative effects of formalin on sperm parameters, chromatin condensation and apoptosis in mice: An experimental study

Ezati

Vardiyan

Talebi

et al. 2020

IJRM

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Background: Formalin is commonly applied as an antiseptic and tissue fixative. It has reactive molecules that lead to its cytotoxic effects. According to recent studies, formalin causes a change in the testicular and sperm structure and L-carnitine (LC) acts as an antioxidant to counteract its effects. Objectives: This study aimed to investigate the protective effects of LC on the parameters, chromatin condensation and apoptosis of mice sperm exposed to formalin. Materials and Methods: In this experimental study, 24 balb/c mice (25-40 gr ,10-12 wk) were divided into three groups (n = 8/each): group I without any injections or gavage; group II, received 10 mg/ kg formalin intraperitoneally (I.P); and group III was exposed to formalin and LC, where a dose of 10 mg/kg formalin was injected I.P daily and LC the dose of 100 mg/kg was kept in a solvent solution. After 31 days, the sperm examination was performed as follows: to evaluate chromatin and DNA quality of the sperm, we applied aniline blue (AB), toluidine blue (TB), chromomycin A3 (CMA3), and terminal transferase-mediated deoxy uridine triphosphate biotin end labeling (TUNEL) tests. Results: Sperm parameters such as count, motility, morphology, and viability displayed a significant decrease in the formalin group. While the data exhibited a considerable augment in sperm parameters in the formalin + LC than the formalin and control groups (p < 0.001), significant differences were detected between groups with respect to TB staining, TUNEL test, CMA3 test and AB staining in the formalin and formalin + LC groups. Conclusion: LC can reduce the negative effects of formalin on sperm parameters, chromatin stability, and percentage of apoptosis in an animal model. Key words: Formalin, L-carnitine, Mice, Sperm chromatin, Apoptosis.

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Superoxide Anion Production by the Spermatozoa of Men with Varicocele: Relationship with Varicocele Grade and Semen Parameters

Cited by 21 publications

References 26 publications

Cross-talk between ER stress and mitochondrial pathway mediated adriamycin-induced testicular toxicity and DA-9401 modulate adriamycin-induced apoptosis in Sprague–Dawley rats

Cross-talk between ER stress and mitochondrial pathway mediated adriamycin-induced testicular toxicity and DA-9401 modulate adriamycin-induced apoptosis in Sprague–Dawley rats

Seminal SIRT1‐oxidative stress relationship in infertile oligoasthenoteratozoospermic men with varicocele after its surgical repair

L-Carnitine reduces the negative effects of formalin on sperm parameters, chromatin condensation and apoptosis in mice: An experimental study

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