Superoxid dismutase activity in portal vein endothelium after partial liver resection

Abstract

PURPOSE:To investigate superoxide dismutase (SOD) activity in the portal vein endothelium and malondialdehyde acid (MDA) production in liver tissue of rats submitted to 70% hepatectomy. METHODS:Twelve rats were distributed in two groups (hepatectomy and sham). Animals were sacrificed on post operative day 1 and portal vein, liver tissue and blood samples were collected. Portal vein SOD production was measured using lucigenin-amplified chemiluminescence assays. MDA measurement was used as an index of oxidative stress through the formation of TBARS (Thiobarbituric Acid Reactive Species). RESULTS:There was no difference in post operative bilirrubin, AST, ALT levels between groups. DHL level was higher in the hepatectomy group (p=0.01). MDA production in the remnant liver tissue and endothelial portal vein SOD activity were also significantly (p<0.05) elevated in the hepatectomy group when compared to control group. There was no correlation between MDA and SOD activity.SOD activity, on the other hand, showed a positive correlation with LDH level (p=0.038) and MDA levels showed a positive correlation with AST and ALT levels (p<0.001). CONCLUSION: There is an increased production of malondialdehyde acid in liver tissue after partial hepatectomy and increased activity of superoxide dismutase in portal vein endothelium as well.

Detection and Quantification of Free Radicals in Peroxisomal Disorders: A Comparative Study with Oxidative Stress Parameters

“…It is a sensitive and reliable marker that indirectly reflects the intensity of cellular injury and ROS production [24]. MDA elevation was considered one of the earliest quantitative disease markers [24].…”

Efeitos do N2-Mercaptopropionilglicina na isquemia e reperfusão hepática em ratos

“…8 Considerando a importância e gravidade das lesões causadas por I/R, diversas drogas foram testadas com o objetivo de minimizar estes efeitos. 7,29,[31][32][33][34][35][36][37][38][39][40][41][42][43] No cenário das moléculas capazes de interferir no processo deletério de I/R apresenta-se o N2-mercaptopropioniglicina (N2-MPG), um composto tiólico sintético, que exerce efeitos benéficos contra as lesões de reperfusão através de suas propriedades varredoras de radicais livres. 14,30 O efeito protetor do N2-MPG já foi testado com sucesso em I/R em ratos com hipertrofia cardíaca 14 , em enxertos cutâneos de ratos 15 , pancreatite aguda experimental 16 , e isquemia e reperfusão hepática em ratos 7…”