Superovulatory response, embryo quality and fertility after treatment with different gonadotrophins in native cattle

Lopes-da-Costa, Luís; Silva, J. Chagas e; Silva, J. Robalo

doi:10.1016/s0093-691x(01)00543-x

Cited by 30 publications

(18 citation statements)

References 49 publications

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“…A single injection of eCG (2,500 IU) is sufficient to induce superovulation because of its long biological half-life (40 h; [1]). But the long half-life of eCG causes continued ovarian stimulation, unovulated follicles and abnormal endocrine profiles, resulting in reduced embryo quality [2][3][4]. In contrast, due to it's short biological half-life (5 h or less), FSH is usually administered in multiple injections (8 to 10 times) twice daily over 4 to 5 days with a total dose of 28 to 50 mg [5][6][7][8][9][10].…”

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“…In contrast, due to it's short biological half-life (5 h or less), FSH is usually administered in multiple injections (8 to 10 times) twice daily over 4 to 5 days with a total dose of 28 to 50 mg [5][6][7][8][9][10]. Some previous reports on superovulation using FSH yielded more transferable embryos compared to eCG [4,6,11]; however, Goulding et al [12] found no differences between FSH-and eCGtreated cows. Although multiple injections of FSH may produce more viable embryos, the multiple injections require more labor and expense and are also more stressful to the donor animals.…”

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The Effect of Reduced Dose and Number of Treatments of FSH on Superovulatory Response in CIDR-Treated Korean Native Cows

Son

Choe

Cho

et al. 2007

Journal of Reproduction and Development

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Abstract. The objective of this study was to investigate the effect of dosage and number of days of follicle stimulating hormone (FSH) treatment on superovulatory response in controlled internal drug release (CIDR)-treated Korean native cows. Forty cows underwent two superovulatory treatments with a crossover design. Cows, at random stages of the estrous cycle, received a CIDR together with injections of 1 mg estradiol benzoate and 50 mg progesterone, and gonadotropin treatment began 4 days later. The cows were divided into 2 groups based on the dosage and number of days of treatment with porcine FSH; a total of 28 mg FSH was given in twice daily intramascular injections in decreasing doses over 4 days (5, 5, 4, 4, 3, 3, 2 and 2 mg; T1 group, n=20) or a total of 24 mg FSH was given in twice daily decreasing doses over 3 days (5, 5, 4, 4, 3 and 3 mg; T2 group, n=20). This was followed by the alternate treatment in the subsequent superovulation. The cows were treated identically in all other respects. PGF2α (25 mg and 15 mg) was given with the 5 th and 6 th injections of FSH, CIDR were withdrawn at the 6 th FSH injection and the cows received 200 µg GnRH 36 h after CIDR withdrawal. The cows were artificially inseminated twice, at 48 and 60 h after CIDR withdrawal, using commercial semen from four Korean native bulls, and embryos were recovered 6 or 7 days after the 2 nd insemination. The numbers of corpora lutea (CL; 7.9 ± 1.0 vs. 8.3 ± 1.1) and large follicles (1.2 ± 0.2 vs. 1.3 ± 0.3) present at the time embryo recovery, as detected by ultrasonography, did not differ between the T1 and T2 groups (P>0.05). Similarly, the numbers of total ova/embryos (6.2 ± 0.9 vs. 6.4 ± 1.1), transferable embryos (3.4 ± 0.8 vs. 3.2 ± 0.7), degenerate embryos (0.8 ± 0.2 vs. 1.0 ± 0.3) and unfertilized ova (2.1 ± 0.5 vs. 2.2 ± 0.5) did not differ between the groups (P>0.05). These data indicate that a reduced dose (24 vs. 28 mg) and number of treatments (6 vs. 8) of FSH for superovulation of CIDR-treated Korean native cows does not affect the embryo yield.

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The Effect of Reduced Dose and Number of Treatments of FSH on Superovulatory Response in CIDR-Treated Korean Native Cows

Son

Choe

Cho

et al. 2007

Journal of Reproduction and Development

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“…The changes in ovarian response have been found to be related with differences in superstimulatory treatments such as total dose, duration and timing of treatment, and the use of additional hormones in the treatment protocol (38,39). Additional factors for the differences in ovarian response might be the genetic of the animal and its environment (3), dietary intake (46), breed (1,9,42), season (33), age (27,29), ovarian status at the time of the treatment (5, 18) and the effects of repeated superstimulations (30). It has been reported that application of FSH in various forms and ways other than im injection can induce superovulatory response and subcunaneous fat amount is considered to be a determinant factor in cattle (10,25).…”

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confidence: 99%

The effect of single epidural plus intramusculer injection of FSH on superovulatory response in Anatolian Black cow

SATILMIŞ¹

2012

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The present study was designed to determine whether a single epidural injection plus intramuscular (im) injection of FSH in order to reduce the number of injection treatments required to induce supurovulatory response, as well as to determine the effects of this treatment on Anatolian Black cow (Bos Taurus). The results will be compared to those obtained by administration of twice daily injections of FSH. Twenty-one Anatolian Black cows were used in this study. Anatolian Black cows received Cue-mate containing 1.56 g progesterone. The cows were divided into three groups (D1, D2 and E) based on the dose and administration route of FSH. In group D1 a total of 490 IU FSH and in group D2 a total of 700 IU FSH was given intramuscularly in decreasing doses for twice daily for 4 days. In both groups superovulatory treatments were initiated on day 7 of the cycle. In group E, FSH was given 175 IU epidural plus 175 IU im injection on day 7 of the cycle. On the 9th day of the cycle, the Cue-mate was removed and the cows received 500 µg prostaglandinF2α in all goups. The cows were artificially inseminated (AI) using semen obtained from native Anatolian Black bulls twice; in the evening of day 11 and in the morning of day 12 with 12 hours intervals. Superovulated cows were non-surgically flushed 7 days after AI. Embryos were morphologically evaluated and classified according to the criteria recommended by the IETS. No significant differences were observed in the mean number of Corpus luteum (CL) among groups which were 3.7 ± 0.56 in D1; 3.4 ± 0.55 in D2 and 6.0 ± 1.14 in E . There was no difference in transferable embryo yield among groups (10/42, 23.8% in D1; 14/32, 43.8% in D2 and 19/82, 23.2% in E). Untransferable embryo yield was not also significantly different among the groups (32/42, 76.2% in D1; 18/32, 56.2% in D2 and 63/82, 76.8% in E). The UFO rate was similar in all groups (17/77, 22.1% in D1; 13/71, 18.3% in D2 and 28/132, 21.2% in E). Recovery rate was found to be significant among groups (59/77, 76.6% in D1; 45/71, 63.4% in D2 and 110/132, 83.3% in E). To conclude, a single epidural injection plus an im injection of FSH application can be obtained acceptable results compared to twice daily injection of FSH for superstimulatory response. However transferable embryo rate of Anatolian Black cow was found to be low in both single epidural injection plus im injection and twice daily FSH applications. Further studies are required to obtain more concrete results on increasing transferable embryo yields in different breeds.Key words: Cow, embryo, epidural injection, FSH, superovulation.İntramuskuler uygulama ile birlikte epidural boşluğa uygulanan FSH'nın Yerli Kara inekte süperovulasyona olan etkisi Özet: Bu çalışma süperovulasyon oluşturmak için tekrarlanan dozlarda yapılan FSH uygulama sayısını azaltmak, FSH'nın tek uygulama şeklinde epidural-im olarak uygulanmasının süperovulasyon oluşturma yönünde etkisini belirlemek ve bu uygulamanın Yerli Kara inekte etkisini ortaya koymak amacıyla tasarlandı. Elde edile...

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“…Estas características parecen favorecer la respuesta superovulatoria con dosis menores de la hormona folículo estimulante (FSH), en comparación a las dosis que se utilizan comercialmente en ganado bovino productor de carne (12)(13)(14)(15) . En México, en la región de la Sierra Madre Occidental que comparten los estados de Durango, Jalisco, Nayarit y Zacatecas existen hatos de animales localmente adaptados denominados Criollo Coreño (4,16) .…”

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Caracterización de la respuesta ovárica a la superovulación en bovino Criollo Coreño utilizando dosis reducidas de FSH

Villaseñor-González

Sánchez

Velázquez

et al. 2017

Rev. Mex. Cienc. Pecu.

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To evaluate the ovarian response to superovulation using reduced doses of FSH in Criollo Coreño cattle two experiments were conducted at the Experimental Station "El Verdineño" (INIFAP) located in Nayarit, México. Twelve (12) cows of 12.4 ± 2.9 yr old (Exp 1) and six heifers of 3.0 ± 0.3 yr old (Exp 2) were used. Three treatments were assigned to each female consisting of 280 mg (T1), 200 mg (T2) and 140 mg (T3) of FSH, so that all females received all treatments. The response variables were transferable embryos (ET), corpuscles retrieved (CR) = (embryos+non fertilized ova), degenerated embryos (ENT), number of corpora lutea (CL), non-fertilized ova (ONF), ovarian volume (VO), serum progesterone (P4), fertilization rate (%F) = ((ET+ENT)*100)/CR) and percent recovery (%R)=CL*100/CR. A cross-over balanced experimental design was used. Analyses were carried out with the GLM procedure of SAS. The statistical model included the fixed effects of treatment (T1, T2 and T3), animal (twelve or six) and period (three periods). In Exp 1 differences among treatments (P<0.05) favorable to T1 were detected for CR and ONF. No differences were found (P>0.05) among treatments for ET, ENT, CL, VO, P4, %F or %R. In Exp 2 no differences were detected (P>0.05) among treatments for any of the variables. It is feasible to use reduced doses of FSH for the superovulation of Criollo Coreño heifers without affecting the response to superovulation or to embryo production.

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Superovulatory response, embryo quality and fertility after treatment with different gonadotrophins in native cattle

Cited by 30 publications

References 49 publications

The Effect of Reduced Dose and Number of Treatments of FSH on Superovulatory Response in CIDR-Treated Korean Native Cows

The Effect of Reduced Dose and Number of Treatments of FSH on Superovulatory Response in CIDR-Treated Korean Native Cows

The effect of single epidural plus intramusculer injection of FSH on superovulatory response in Anatolian Black cow

Caracterización de la respuesta ovárica a la superovulación en bovino Criollo Coreño utilizando dosis reducidas de FSH

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