Superovulation in the Mare: A Work in Progress

Roser, Janet F.; Meyers-Brown, Geraldine

doi:10.1016/j.jevs.2012.05.055

Cited by 15 publications

(7 citation statements)

References 104 publications

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“…More recent successful applications of such two-step protocols include mature human oocytes (Kuwayama et al 2005). However, for horse oocytes, despite good maturation rates (Rosati et al 2012;de Leon et al 2012) and some blastocyst development from equine vitrified oocytes, the relatively long exposure to CPAs was associated with CPA toxicity, affecting subsequent embryo development (Canesin et al 2017). Tharasanit et al (2006aTharasanit et al ( , 2006b) tried vitrification of equine oocytes after only 30 s exposure to the equilibration solution followed by 25 s in the vitrification solution.…”

Section: Equilibration Solutionmentioning

confidence: 99%

Cryopreservation of equine oocytes: looking into the crystal ball

Coster

Angel‐Velez

Soom

et al. 2020

Reprod. Fertil. Dev.

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In vitro embryo production has evolved rapidly in the horse over the past decade, but blastocyst rates from vitrified equine oocytes remain quite poor and further research is needed to warrant application. Oocyte vitrification is affected by several technical and biological factors. In the horse, short exposure of immature oocytes to the combination of permeating and non-permeating cryoprotective agents has been associated with the best results so far. High cooling and warming rates are also crucial and can be obtained by using minimal volumes and open cryodevices. Vitrification of in vivo-matured oocytes has yielded better results, but is less practical. The presence of the corona radiata seems to partially protect those factors that are necessary for the construction of the normal spindle and for chromosome alignment, but multiple layers of cumulus cells may impair permeation of cryoprotective agents. In addition to the spindle, the oolemma and mitochondria are also particularly sensitive to vitrification damage, which should be minimised in future vitrification procedures. This review presents promising protocols and novel strategies in equine oocyte vitrification, with a focus on blastocyst development and foal production as most reliable outcome parameters.

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Section: Equilibration Solutionmentioning

confidence: 99%

Cryopreservation of equine oocytes: looking into the crystal ball

Coster

Angel‐Velez

Soom

et al. 2020

Reprod. Fertil. Dev.

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“…In some species, response to superovulation is very poor. In mares (Roser and Meyers-Brown 2012 ) and water buffalo (Drost 2007 ), for instance, embryo recovery after superovulation is often less than after natural ovulation. In the pig, in which the high natural ovulation rate limits the need for superovulation, embryonic viability can sometimes be reduced by superovulation ( Youngs 2011 ).…”

Section: Embryo Transfermentioning

confidence: 99%

Current and Future Assisted Reproductive Technologies for Mammalian Farm Animals

Hansen

2013

Advances in Experimental Medicine and Biology

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Reproduction in domestic animals is under control by man and the technologies developed to facilitate that control have a major impact on the efficiency of food production. Reproduction is an energy-intensive process. In beef cattle, for example, over 50 % of the total feed consumption required to produce a unit of meat protein is consumed by the dam of the meat animal (Anim Prod 27:367-379, 1978). Sows are responsible for about 20 % of the total feed needed to produce animals for slaughter (Adv Pork Prod 19:223-237, 2008). Accordingly, energy input to produce food from animal sources is reduced by increasing number of offspring per unit time a breeding female is in the herd. Using beef cattle as an example again, life-cycle efficiency for production of weaned calves is positively related to early age at puberty and short calving intervals (J Anim Sci 57:852-866, 1983). Reproductive technologies also dictate the strategies that can be used to select animals genetically for traits that improve production. Of critical importance has been artificial insemination (AI) (Anim Reprod Sci 62:143-172, 2000; Stud Hist Philos Biol Biomed Sci 38:411-441, 2007; Reprod Domest Anim 43:379-385, 2008; J Dairy Sci 92:5814-5833, 2009) and, as will be outlined in this chapter, emerging technologies offer additional opportunities for improvements in genetic selection. Given the central role of reproduction as a determinant of production efficiency and in genetic selection, improvements in reproductive technologies will be crucial to meeting the challenges created by the anticipated increases in world population (from seven billion people in 2011 to an anticipated nine billion by 2050; World population prospects: the 2010 revision, highlights and advance tables. Working Paper No. ESA/P/WP.220, New York) and by difficulties in livestock production wrought by climate change (SAT eJournal 4:1-23, 2007).The purpose of this chapter will be to highlight current and emerging reproductive technologies that have the potential to improve efficiency of livestock production. The focus will be on technologies that manipulate male and female gametes as well as the stem cells from which they are derived and the preimplantation embryo. While technology is crucial to other interventions in the reproductive process like control of seasonal breeding, hormonal regulation of ovulation, estrous cyclicity and pregnancy establishment, feeding to optimize reproduction, minimizing environmental stress, and selection of genes controlling reproduction, these will not be considered here. Rather the reader is directed to other chapters in this volume as well as some reviews on other aspects of artificial manipulation of reproduction (Reprod Fertil Dev 24:258-266, 2011; Reprod Domest Anim 43:40-47, 2008; Reprod Domest Anim 43:122-128, 2008; Soc Reprod Fertil Suppl 66:87-102, 2009; Comprehensive biotechnology, Amsterdam, pp 477-485; Dairy production medicine, Chichester, pp 153-163; Theriogenology 76:1619-1631, 2011; Theriogenology 76:1568-1582, 2011; Theriogenology...

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“…In vitro equine embryo production following intracytoplasmic sperm injection (ICSI) and in vitro embryo culture is currently used worldwide, both for research and clinical production of foals (Galli et al 2007, Claes et al 2019. Superovulation remains problematic in the horse (Roser & Meyers-Brown 2012), meaning that recovery of immature oocytes followed by in vitro oocyte maturation (IVM) is typically used to maximise the number of mature oocytes available for fertilisation. The most widely used media for equine IVM are M199 (Zhang et al 1989) and DMEM/F-12, (Galli et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Glucose concentration during equine in vitro maturation alters mitochondrial function

et al. 2020

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The use of in vitro embryo production in the horse is increasing in clinical and research settings; however, protocols are yet to be optimised. Notably, the two most commonly used base media for in vitro maturation (IVM) supply glucose at markedly different concentrations: physiological (5.6 mM, M199) or supraphysiological (17 mM, DMEM/F-12). Exposure to high glucose has detrimental effects on oocytes and early embryos in many mammalian species, but the impact has not yet been examined in the horse. To address this, we compared the energy metabolism of equine COCs matured in M199-based maturation medium containing either 5.6 or 17 mM glucose, as well as expression of key genes in oocytes and cumulus cells. Oocytes were fertilised by ICSI and cultured. Analysis of spent medium revealed that COC glucose consumption and production of lactate and pyruvate were similar between treatments. However, the glycolytic index was decreased at 17 mM and analysis of mitochondrial function of COCs revealed that IVM in 17 mM glucose was associated with decreased ATP-coupled respiration and increased non-mitochondrial respiration compared to that for 5.6 mM glucose. We also found that the metabolic enzyme lactate dehydrogenase-A (LDHA) was downregulated in cumulus cells of oocytes that completed IVM in 17 mM glucose. There was no difference in maturation or blastocyst rates. These data indicate that COC mitochondrial function and gene expression are altered by high glucose concentration during IVM. Further work is needed to determine if these changes are associated with developmental changes in the resulting offspring.

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Superovulation in the Mare: A Work in Progress

Cited by 15 publications

References 104 publications

Cryopreservation of equine oocytes: looking into the crystal ball

Cryopreservation of equine oocytes: looking into the crystal ball

Current and Future Assisted Reproductive Technologies for Mammalian Farm Animals

Glucose concentration during equine in vitro maturation alters mitochondrial function

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