Superovulation in Beef Cows and Heifers with a Single Injection of FSH dissolved in Polyvinylpyrrolidone.

Yamamoto, Masao; Suzuki, Takahiro; Ooe, Masato; Takagi, Mitsuhiro

doi:10.1262/jrd.39.353

Cited by 9 publications

(6 citation statements)

References 8 publications

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“…In a previous study of superovulation induced by a single im administration of pFSH in Japanese Black cows, 30 mg of pFSH dissolved in 30% PVP induced multiple ovulations and produced embryos, whereas a single im administration of 30 mg of pFSH dissolved in 10 mL of saline stimulated multiple follicular growth but did not lead to ovulation [13]. In contrast, the present study showed that it was possible to induce a superovulatory response when pFSH was administered subcutaneously, and the results were similar to those obtained previously with sc administration [22].…”

Section: Discussionmentioning

confidence: 92%

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Superovulatory response in Japanese Black cows receiving a single subcutaneous porcine follicle–stimulating hormone treatment or six intramuscular treatments over three days

Hiraizumi

Nishinomiya

Oikawa³

et al. 2015

Theriogenology

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Section: Discussionmentioning

confidence: 92%

“…Polyvinylpyrrolidone (PVP) is a diluent polymer suitable for prolonging the absorption of pFSH after a single intramuscular (im) administration [12][13][14][15][16][17][18]. However, PVP is viscous and difficult to mix with pFSH.…”

Section: Introductionmentioning

confidence: 99%

Superovulatory response in Japanese Black cows receiving a single subcutaneous porcine follicle–stimulating hormone treatment or six intramuscular treatments over three days

Hiraizumi

Nishinomiya

Oikawa³

et al. 2015

Theriogenology

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“…Then it was divided into aliquots of 10 ml each and stored at 4 C. Just before use, FSH (Folltropin ® -V; Bioniche Animal Health, Belleville, ON, Canada) was dissolved at 20 mg/ml in diluent (Folltropin ® -V Diluent; Bacteriostatic Sodium Chloride Injection, Bioniche Animal Health) and mixed well with 10 ml of 30% PVP solution to create pFSH [11]. …”

Section: Methodsmentioning

confidence: 99%

“…Before FSH treatments, the estrus cycles of 16 Thai native heifers (2.13 ± 0.14 years old and 187.52 ± 1.82 kg; mean ± SEM) were synchronized by injecting 500 μg PGF 2α (Cloprostenol, Estrumate; Coopers, Berkhamsted, England) twice at an interval of 12 days. Follicular growth in heifers was stimulated by injection of pFSH between days 9 and 12 of the estrous cycle (day of estrus = day 0) [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

“…To prepare follicle stimulating hormone dissolved in PVP (FSHp), firstly 30 g of PVP (PVP-40, molecular weight 40,000; Sigma-Aldrich Chemical, St. Louis, MO, USA) was dissolved in 100 ml of distilled water to produce a 30% (wt/vol) PVP solution and sterilized by autoclaving. Then it was divided into aliquots of 10 ml each and stored at 4 C. Just before use, FSH (Folltropin ® -V; Bioniche Animal Health, Belleville, ON, Canada) was dissolved at 20 mg/ml in diluent (Folltropin ® -V Diluent; Bacteriostatic Sodium Chloride Injection, Bioniche Animal Health) and mixed well with 10 ml of 30% PVP solution to create pFSH [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

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Superstimulation of Follicular Growth in Thai Native Heifers by a Single Administration of Follicle Stimulating Hormone Dissolved in Polyvinylpyrrolidone

Chasombat

Sakhong

Nagai

et al. 2013

Journal of Reproduction and Development

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This study was undertaken to determine whether a single i.m. injection of FSH dissolved in 10 ml of 30% (wt/vol) polyvinylpyrrolidone (PVP; MW=40,000) to form FSHp would induce follicular growth in Thai native heifers and to determine its optimal dose. In Group 1, heifers (n=4) were given multiple i.m. injections of FSHp every 12 h for 3 days at decreasing doses, for a total of 100 mg (control). In Groups 2, 3 and 4, heifers (n=4 in each group) were given single i.m. injections of FSHp at 50, 100 and 150 mg. All heifers received a single injection of 750 µg PGF2α 48 h after the initiation of exogenous FSH treatment. Ovaries of treated heifers were examined by transrectal ultrasonography every day until they showed estrus. Group 3 showed significantly higher numbers of ovulation follicles, significantly higher growth rates of follicles per day and significantly larger diameters of follicles and corpora lutea than groups 1 and 2 but not Group 4 (P<0.05). Group 4 showed significantly higher numbers of large follicles (≥5 mm in diameter), unovulated follicles and ovulations, a significantly higher growth rate of follicles per day, and significantly larger diameters of follicles and corpora lutea (P<0.05) than those of the other groups. This indicates a state of overstimulation of ovaries in this group. Besides, the plasma levels of FSH in Group 4 were significantly higher (P<0.05) than in the other group and were maintained in the range of 2.2–0.7 ng/ml over a period of 6 to 66 h after the FSHp injection. Meanwhile, the plasma levels of P4 and E2 did not differ in any of the groups in the period of 0 to 96 h during the superstimulation program. In conclusion, it was demonstrated that a single i.m. injection of 100 mg FSHp was the most effective dose for superstimulation of follicular growth in Thai native heifers under the experimental conditions in this study.

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Responsiveness of rabbits to superovulation treatment by a single injection of follicle‐stimulating hormone with aluminum hydroxide gel

Hashimoto¹,

Kimura

Kuramochi³

et al. 2007

Molecular Reproduction Devel

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Aluminum hydroxide gel (Al-gel), which is used as an adjuvant, can absorb macromolecules. We investigated the applicability of Al-gel to the sustained release of follicle-stimulating hormone (FSH) as a simplified method of superovulation (SOV) in rabbits. The responsiveness of rabbits to SOV by a single injection of FSH dissolved in Al-gel suspension (3.2 mg Al/ml) and in 10% (w/v) polyvinylpyrrolidone (PVP), and by multiple injections of FSH in saline was examined. The numbers of total and fertilized eggs recovered from rabbits treated with FSH in Al-gel (40.5 and 26.3, respectively) were similar to multiple injections (47.4 and 28.6, respectively) and were significantly greater (P < 0.05) than single injection of FSH with PVP (17.3 and 11.5, respectively). We also compared the plasma FSH levels of rabbits which were induced SOV by multiple or a single injection of Al-gel. Al-gel provided sustained release of FSH to the blood stream at a high enough dose for SOV. Moreover, the developmental competence of the pups of DNA-injected embryos from rabbits treated with a single injection of FSH mixed with Al-gel (18%) was similar to that of DNA-injected embryos, recovered from rabbits treated with FSH dissolved in saline (21%). Two transgenic pups were obtained from embryos recovered from rabbits by a single injection of FSH with Al-gel. These results indicate that a single injection of FSH with Al-gel is an effective method for SOV of rabbit and that this technique is applicable to research requiring large numbers of rabbit embryos such as the production of transgenic rabbits.

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Superovulation in Beef Cows and Heifers with a Single Injection of FSH dissolved in Polyvinylpyrrolidone.

Cited by 9 publications

References 8 publications

Superovulatory response in Japanese Black cows receiving a single subcutaneous porcine follicle–stimulating hormone treatment or six intramuscular treatments over three days

Superovulatory response in Japanese Black cows receiving a single subcutaneous porcine follicle–stimulating hormone treatment or six intramuscular treatments over three days

Superstimulation of Follicular Growth in Thai Native Heifers by a Single Administration of Follicle Stimulating Hormone Dissolved in Polyvinylpyrrolidone

Responsiveness of rabbits to superovulation treatment by a single injection of follicle‐stimulating hormone with aluminum hydroxide gel

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