Superovulation and in vitro oocyte maturation in three species of mice (Mus musculus, Mus spretus and Mus spicilegus)

Martin-Coello, Juan; González, Raquel; Crespo, C.; Gomendio, Montserrat; Roldán, Eduardo R. S.

doi:10.1016/j.theriogenology.2008.06.002

Cited by 36 publications

(47 citation statements)

References 54 publications

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“…In mice, treatment with an eCG-hCG combination is the most reliable and the regimen most frequently used for inducing superovulation at any stage of the estrous cycle, although there is a great strain-dependent variation ranging from 5.4 to 39.5 oocytes per female [16]. Wild species such as M spretus and M spicilegus are especially poor responders to this conventional superovulation regimen [17], and in this study, we confirmed the fact that females of the wild-derived subspecies M musculus molossinus were also poor responders. To circumvent the difficulty in collecting mature oocytes from these mice, we attempted to superovulate them by passive immunization with AIS [18][19][20], which is known to stimulate folliculogenesis in different mammalian species, including mice, rats, hamsters, guinea pigs, cows, mares, ewes, and goats [7].…”

Section: Discussionmentioning

confidence: 99%

26. Equilibrium vitrification of mouse embryos

et al. 2009

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Section: Discussionmentioning

confidence: 99%

26. Equilibrium vitrification of mouse embryos

et al. 2009

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“…Mus musculus and Mus musculus x Mus spretus F1 embryos were obtained by IVF following a previously described method with minor modifications (Martín-Coello et al 2008). Spermatozoa were collected from the epididymis of either young (3 months) or old (1 year) Mus musculus C57CBAF1 mature fertile males or Mus spretus males and were pre-incubated in a 500 ml drop of human tubular fluid (HTF) (Quinn et al 1985) medium supplemented with 2 mg/ml BSA under oil at 37 8C in 5% CO 2 /air for 15 min to allow sperm dispersion.…”

Section: Production Of Mouse Embryosmentioning

confidence: 99%

Spermatozoa telomeres determine telomere length in early embryos and offspring

Frutos¹,

López‐Cardona²,

Balvís³

et al. 2016

Reproduction

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Offspring telomere length (TL) has been correlated with paternal TL, but the mechanism for this parent of origin-specific inheritance remains unclear. The objective of this study has been to determine the role of spermatozoa TL in embryonic telomere lengthening by using two mouse models showing dimorphism in their spermatozoa TL: Mus musculus vs Mus spretus and old vs young Mus musculus. Mus spretus spermatozoa displayed a shorter TL than Mus musculus. Hybrid offspring exhibited lower TL compared with Mus musculus starting at the two-cell stage, before the onset of telomerase expression. To analyze the role of spermatozoa telomeres in early telomere lengthening, we compared the TL in oocytes, zygotes, two-cell embryos and blastocysts produced by parthenogenesis or by fertilization with Mus musculus or Mus spretus spermatozoa. TL was significantly higher in spermatozoa compared with oocytes, and it increased significantly from the oocyte to the zygote stage in those embryos fertilized with Mus musculus spermatozoa, but not in those fertilized with Mus spretus spermatozoa or produced by parthenogenesis. A further increase was noted from the zygote to the two-cell stage in fertilized Mus musculus embryos, whereas hybrid embryos maintained the oocyte TL. Spermatozoa TL shortened with age in Mus musculus and the offspring from young males showed a significantly higher TL compared with that fathered by old males. These significant differences were already noticeable at the two-cell stage. These results suggest that spermatozoa telomeres act as a guide for telomerase-independent telomere lengthening resulting in differences in TL that persist after birth. Free Spanish abstract: A Spanish translation of this abstract is freely available at

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“…小鼠因其繁殖率高、繁殖周期短等特点被常用于生物医学研究, 尤其是胚胎生物学和胚胎工程研究 (Brooke et al, 2007;Ding et al, 2007;Martín-Coello et al, 2008)。超数排卵技术作为在同一时间段获得足够数量卵母细胞或胚胎的主要手段之一, 是开展胚胎生物学和胚胎工程研究的基础 (Chen et al, 1991;He et al, 1999He et al, , 2005。小鼠超排效果除受小鼠日龄和超排激素剂量影响之外 (Cui et al, 2012;Yang, 2007), 主要受超排注射促性腺激素时小鼠所处发情周期阶段的影响, 但国内外对于小鼠超排的适合发情周期阶段所获得的研究结果并不一致，如 Fu et al (2005 细胞以白细胞为主, 间或少量中间层细胞和完全角化上皮细胞。白细胞形态小而圆, 细胞核较大, 为马蹄状或分叶状, 深紫色; 细胞质为浅紫红色。中间层细胞呈鳞状, 可见深蓝色圆形细胞核, 胞质为 …”

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