Supernates from stored red blood cells inhibit platelet aggregation

Matheu, Frederick A; McFaul, Steve J.

doi:10.1111/j.1537-2995.2010.02582.x

Cited by 9 publications

(3 citation statements)

References 27 publications

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“…By studying reconstituted whole blood instead of unmixed platelet concentrates, the interaction with RBCs and plasma, resembling the clinical situation of a transfused patient more closely, is also taken into account in our study. Multiple in vitro and in vivo studies have indicated that RBCs and plasma affect platelet function and haemostasis . When stored platelets are ex vivo mixed with whole blood, the agonist‐induced CD62P expression and aggregation improve , which implies the influence of RBCs and/or plasma on platelet function.…”

Section: Discussionmentioning

confidence: 99%

Comparison of haemostatic function of PAS‐C–platelets vs. plasma–platelets in reconstituted whole blood using impedance aggregometry and thromboelastography

et al. 2017

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Section: Discussionmentioning

confidence: 99%

Comparison of haemostatic function of PAS‐C–platelets vs. plasma–platelets in reconstituted whole blood using impedance aggregometry and thromboelastography

et al. 2017

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“…Mounting evidence suggests that RBC transfusions modify hemostasis and thrombosis, supported by their association with substantial increases in both venous and arterial thrombosis in epidemiologic studies 9 . Therefore, it is likely that stored RBCs and supernatant mediators alter recipient PLT function 56 . Indeed, an in vitro transfusion model demonstrated that RBC transfusions affect PLT function in healthy adults 57 .…”

Section: Pltsmentioning

confidence: 99%

New frontiers in transfusion biology: identification and significance of mediators of morbidity and mortality in stored red blood cells

et al. 2011

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Red cell transfusions are associated with inflammation and thrombosis, both arterial and venous, the mechanisms of which are not understood. Although a necessary life saving procedure in modern medicine, transfusions have rarely been subjected to modern assessments of efficacy and safety, including randomized trials. Storage of red blood cells induces changes, including the release of free hemoglobin and the accumulation of biologically active soluble mediators and microparticles. These mediators likely play a direct role in the inflammatory and pro-thrombotic properties of red cell transfusions. Methods such as leukoreduction, washing of red cells and rejuvenation may improve the quality of red cell transfusions.

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“…An in vitro study suggested that the platelets in fresh WB were more reactive with collagen and may therefore be more able to adhere at the site of injury and participate in primary platelet plug formation than those stored for extended periods . Additionally, stored supernatants of red cells have been shown to inhibit platelet aggregation .…”

Section: Introductionmentioning

confidence: 99%

An in vitro study of coagulation properties in refrigerated whole blood and reconstituted whole blood

Wang

Zhao

et al. 2019

Vox Sanguinis

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Objective Fresh whole blood (WB) has been used in military applications and cardiac surgery. We undertook a study of the coagulation properties of refrigerated WB stored for 21 days and compared them with the properties of reconstituted WB. Study design and methods Ten WB units were obtained from healthy volunteer donors and stored at 4 ± 2°C. Samples were obtained on Days 1, 2, 4, 6, 8, 10, 14 and 21 from the WB units. Ten units of reconstituted WB were prepared with a ratio of red cells, platelets and plasma of 1:1:1. Tests included complete blood count, electrolyte, routine coagulation, blood coagulation factor and thromboelastography. Results There was a progressive decline in Hb, WBC, PLT, sodium and coagulation factors but a progressive increase in APTT, PT and potassium in WB. The concentrations of factor (F)V and FVIII as well as FII and FX of WB were higher before Days 4, 2, 8 and 14, respectively, compared with the concentrations of reconstituted WB. The concentrations of FVII, FIX, FXI and FXII in WB were found to be equal to or higher than those in reconstituted WB throughout the course of 21 days. TEG variables in all WB units were normal throughout the course of 10 days. The mean PT and APTT of WB were lower than those of reconstituted WB before Days 14 and 10, respectively. Conclusion This study suggests that the coagulation properties of refrigerated WB were equal to or superior to those of reconstituted WB for a minimum of 10 days.

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Supernates from stored red blood cells inhibit platelet aggregation

Abstract: Substances that decrease expression of GPIIb/IIIa and inhibit PLT aggregation accumulate in NLR RBCs. Accumulation of this material is prevented by leukoreduction.

Cited by 9 publications

References 27 publications

Comparison of haemostatic function of PAS‐C–platelets vs. plasma–platelets in reconstituted whole blood using impedance aggregometry and thromboelastography

Comparison of haemostatic function of PAS‐C–platelets vs. plasma–platelets in reconstituted whole blood using impedance aggregometry and thromboelastography

New frontiers in transfusion biology: identification and significance of mediators of morbidity and mortality in stored red blood cells

An in vitro study of coagulation properties in refrigerated whole blood and reconstituted whole blood

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