Superior performance and mechanism of chlorobenzene degradation by a novel bacterium

Zhang, Shihan; Ying, Zanyun; You, Juping; Ye, Jiexu; Cheng, Zhuowei; Chen, Dongzhi; Chen, Jianmeng

doi:10.1039/c9ra01229j

Cited by 14 publications

(12 citation statements)

References 52 publications

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“…Part of CB is decomposed into Cl − by a ring‐opening reaction, and benzene generates phenol 45 . The other part is decomposed into O/M/P‐chlorophenol by ortho‐ring cleavage, further transformed into 3‐catechol, and oxidized to acyl chloride 29,46 . During oxidation, ring‐opening dechlorination and chain‐breaking reactions occur to produce oxalic acid, acetic acid, and other substances.…”

Section: Resultsmentioning

confidence: 99%

“…Bacterial solution was spread in solid medium, then incubated at a constant temperature of 30°C for 48 h. To obtain pure cultures, colonies were subjected to transfer twice on agar plates. The determination of the strain degradation ability was to pick the colony and reinoculate it into a serum bottle containing CBs to analyze CB concentration 29 . The selection of strain was based on its superior capability of the CB reduction efficiency above 85% within 8 h.…”

Section: Methodsmentioning

confidence: 99%

“…The determination of the strain degradation ability was to pick the colony and reinoculate it into a serum bottle containing CBs to analyze CB concentration. 29 The selection of strain was based on its superior capability of the CB reduction efficiency above 85% within 8 h.…”

Section: Isolation and Screening Of Strainsmentioning

confidence: 99%

“…45 The other part is decomposed into O/M/Pchlorophenol by ortho-ring cleavage, further transformed into 3-catechol, and oxidized to acyl chloride. 29,46 During oxidation,…”

Section: Degradation Pathway Of Cbmentioning

confidence: 99%

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Reduction characteristic of chlorobenzene by a newly isolated Paenarthrobacter ureafaciens LY from a pharmaceutical wastewater treatment plant

Liu,

Yao,

Zhang

et al. 2024

Cell Biochemistry & Function

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A highly efficient chlorobenzene‐degrading strain was isolated from the sludge of a sewage treatment plant associated with a pharmaceutical company. The strain exhibited a similarity of over 99.9% with multiple strains of Paenarthrobacter ureafaciens. Therefore, the strain was suggested to be P. ureafaciens LY. This novel strain exhibited a broad spectrum of pollutant degradation capabilities, effectively degrading chlorobenzene and other organic pollutants, such as 1, 2, 4‐trichlorobenzene, phenol, and xylene. Moreover, P. ureafaciens LY co‐metabolized mixtures of chlorobenzene with 1, 2, 4‐trichlorobenzene or phenol. Evaluation of its degradation efficiency showed that it achieved an impressive degradation rate of 94.78% for chlorobenzene within 8 h. The Haldane–Andrews model was used to describe the growth of P. ureafaciens LY under specific pollutants and its concentrations, revealing a maximum specific growth rate (μmax) of 0.33 h−1. The isolation and characterization of P. ureafaciens LY, along with its ability to degrade chlorobenzene, provides valuable insights for the development of efficient and eco‐friendly approaches to mitigate chlorobenzene contamination. Additionally, investigation of the degradation performance of the strain in the presence of other pollutants offers important information for understanding the complexities of co‐metabolism in mixed‐pollutant environments.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Isolation and Screening Of Strainsmentioning

confidence: 99%

“…45 The other part is decomposed into O/M/Pchlorophenol by ortho-ring cleavage, further transformed into 3-catechol, and oxidized to acyl chloride. 29,46 During oxidation,…”

Section: Degradation Pathway Of Cbmentioning

confidence: 99%

See 2 more Smart Citations

Reduction characteristic of chlorobenzene by a newly isolated Paenarthrobacter ureafaciens LY from a pharmaceutical wastewater treatment plant

Liu,

Yao,

Zhang

et al. 2024

Cell Biochemistry & Function

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“…The chlorobenzene remediation technologies mainly include gas thermal remediation (Xu et al 2019), advanced oxidation (Xie et al 2020) and microbial degradation (Zhang et al 2019). Thermal desorption technology is energy-intensive and costly, and the soil temperature can still be as high as 70°C after a week of restoration, which seriously damages the native environment of the soil (Xu et al 2019).…”

Section: Introductionmentioning

confidence: 99%

A Pandoraea sp. strain efficiently degrades chlorobenzene via monooxygenation pathways with high potential for groundwater bioremediation

Pan,

Yuan,

et al. 2024

Preprint

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Chlorobenzene (CB) is often detected in contaminated soil and groundwater at in-service petrochemical facilities. Given the high safety requirements for these petrochemical facilities, a sustainable and low-consumption microbial remediation technology is preferred. The aim of this study was to isolate an efficient chlorobenzene-degrading bacterial strain and understand its degradation mechanism to be used for in-situ bioremediation of chlorobenzene-contaminated sites in in-service petrochemical enterprises. A degrading bacterium was isolated from chlorobenzene-contaminated soil at a pesticide plant, identified as Pandoraea sp. XJJ-1 (CCTCC M 2021057). This strain completely degraded 100 mg·L− 1 CB and showed extensive degradability across a range of pH (5.0–9.0), temperature (10°C-37°C), and CB concentrations (100–600 mg·L− 1). Notably, the degradation efficiency was 85.2% at 15°C, and the strain could also degrade six other aromatic hydrocarbons, including benzene, toluene, ethylbenzene, and xylene (o-, m-, p-). The metabolic pathway of chlorobenzene was inferred using Ultra Performance Liquid Chromatography (UPLC), Gas chromatography-mass spectrometry (GC-MS), and genomic analysis. In strain XJJ-1, CB was metabolized to o-chlorophenol and 3-chloroxychol by chlorobenzene monooxygenase, followed by ortho-cleavage by the action of 3-chlorocatechol 1,2-dioxygenase. This is the first report of chlorobenzene monooxygenase as the rate-limiting step in Pandoraea spp.. Strain XJJ-1, which exhibits excellent degradation ability for CB at low temperatures, was isolated in this study. Moreover, the presence of the chlorobenzene monooxygenation pathway metabolism in strain XJJ-1 is reported for the first time in Pandoraea. As a biomaterial with low temperature resistance and composite pollutant degradation capacity, strain XJJ-1 has potential applications prospects in the in-situ bioremediation of chlorobenzene-contaminated sites.

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Efficient biodegradation of chlorobenzene via monooxygenation pathways by Pandoraea sp. XJJ-1 with high potential for groundwater bioremediation

Pan,

Yuan,

et al. 2024

Int Microbiol

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Superior performance and mechanism of chlorobenzene degradation by a novel bacterium

Abstract: A newly isolated strain was identified as Ochrobactrum sp. by 16S rRNA sequence analysis and named as ZJUTCB-1.

Cited by 14 publications

References 52 publications

Reduction characteristic of chlorobenzene by a newly isolated Paenarthrobacter ureafaciens LY from a pharmaceutical wastewater treatment plant

Reduction characteristic of chlorobenzene by a newly isolated Paenarthrobacter ureafaciens LY from a pharmaceutical wastewater treatment plant

A Pandoraea sp. strain efficiently degrades chlorobenzene via monooxygenation pathways with high potential for groundwater bioremediation

Efficient biodegradation of chlorobenzene via monooxygenation pathways by Pandoraea sp. XJJ-1 with high potential for groundwater bioremediation

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