Superior myocardial preservation with modified UW solution after prolonged ischemia in the rat heart

Yeh, Thomas; Sa, Hanan; de, J.P. Marques; Im, Rebeyka; As, Abd-Elfattah; Kf, Lee; As, Wechsler

doi:10.1016/0003-4975(90)90869-8

Cited by 44 publications

(11 citation statements)

References 12 publications

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“…With the lactobionic acidbased preservation solution, the liver can be preserved extended lung preservation in a large animal orsuccessfully for up to 48 h in laboratory studies, the thotopic model will be carried out at the University pancreas and kidney for up to 72 h, and the heart for of Minnesota. These plans involve using on a swine up to 12 h [9][10][11][12]. orthotopic lung transplantation model to focus on Hydroxyl starch is another additive to UW which evaluating the long-term pulmonary function of the produces colloid osmotic pressure, thus helping prevent transplanted lung.…”

Section: Discussionmentioning

confidence: 99%

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Comparative Study of Solutions for Pulmonary Preservation Using an Isolated Rabbit Lung Model

Al‐Dossari

Shumway

1998

Journal of Surgical Research

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Section: Discussionmentioning

confidence: 99%

“…The heart-UW solution, used for preserving livers, pancreases, lung blocks of donor rabbits were flushed with, and hearts, and kidneys, has permitted successful longthen preserved in, one of the solutions at 4ЊC. Five rab-term allograft preservation [9][10][11][12][13][14]. Using UW solution bits were studied in each group.…”

mentioning

confidence: 99%

Comparative Study of Solutions for Pulmonary Preservation Using an Isolated Rabbit Lung Model

Al‐Dossari

Shumway

1998

Journal of Surgical Research

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“…Storage solutions (Table I) 24,30,40, and 48 hours of preservation to assess the length of protection afforded by these modifications.…”

Section: Methodsmentioning

confidence: 99%

Prolonging myocardial preservation with a modified University of Wisconsin solution containing 2,3-butanedione monoxime and calcium

Stringham

Paulsen

Southard

et al. 1994

The Journal of Thoracic and Cardiovascular Surgery

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solution is an effective preservative for cold storage of the cardiac aUograft. In an earlier study we showed that addition of calcium and 2,3-butanedione monoxime, a reversible inhibitor of myocardial contracture, further improved preservation of the rabbit heart. In this study we investigated the foUowing: (1) the effects of different concentrations of 2,3-butanedione monoxime and calcium on function of the preserved rabbit heart, (2) how heart preservation is affected when 2,3-butanedione monoxime and calcium are added to the St. Thomas' Hospital and Stanford solutions, and (3) how 2,3-butanedione monoxime and calcium, at optimal concentrations in University of Wisconsin solution, affect hearts preserved up to 48 hours. Rabbit hearts were flushed with preservative and stored at 4°C for 24, 30, 40, or 48 hours. Myocardial function was assessed during 60 minutes of isolated reperfusion, and myocardial adenine nucleotide content was measured after completion of reperfusion. Three concentrations of 2,3-butanedione monoxime (15, 30, and 60 mmolj L) in the University of Wisconsin solution were studied in hearts preserved for 30 hours. Storage with 2,3-butanedione monoxime at 30 mmoljL resulted in significantly better left ventricular developed pressure (p < 0.01), left ventricular end-diastolic volume (p < 0.01), rate of left ventricular pressure rise (p < 0.01), coronary flow (p < 0.05), rate-pressure product (p < 0.001), and adenine nucleotide regeneration (p < 0.05) than with 60 mmoljL, although function was not significantly different when the osmolarity of the solutions was equalized. There was significant reduction in end-diastolic volume (p <0.05) and adenine nucleotide recovery (p <0.01) when 2,3-butanedione monoxime was lowered to 15 mmoljL. Decreasing the calcium concentration from 1.0 to 0.1 mmoljL also had a deleterious effect on myocardial function (p < 0.05). The addition of 30 mmoljL 2,3-butanedione monoxime and 1.0 mmoljL calcium to the St. Thomas' or Stanford solutions improved preservation of the heart when compared with the unmodified solutions, but to a lesser degree than with the modified University of Wisconsin solution. After 24 to 48 hours of storage in University of Wisconsin solution containing 30 mmol/L 2,3-butanedione monoxime and 1.0 mmoljL calcium, there was substantial improvement in developed pressure (p < 0.001), end-diastolic volume (p < 0.05), and rate pressure product (p < 0.001), although there was little effect on heart rate and coronary flow, when compared with the unmodified University of Wisconsin solution. We conclude that modification of the University of Wisconsin solution with 30 mmoljL 2,3-butanedione monoxime and 1.0 mmoljL calcium significantly improves long-term preservation of the rabbit heart. Further extension in preservation might be accomplished by optimizing the osmolarity of this modified solution.

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“…The large impermeants that have osmotic actions in this solution may play a role in extending the safe period for myocardial storage without excessive reperfusion edema. 27 Whether abnormalities of cell volume expansion are primary in creating myocardial injury or whether there is an additive effect as a consequence of the excessive energy necessary to restore cell volume is unknown, but the problem can be addressed.…”

Section: Myocardial Edemamentioning

confidence: 99%

Future Cardioprotective Considerations

Wechsler

Abd‐Elfattah

1993

J Cardiac Surgery

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Remarkable strides have been made in perioperative myocardial protection for heart operations. Recent advances in understanding the physiology of myocardial ischemia and its protective responses suggest that there is a possibility for further improvement. Some of these strategies are discussed in this article, which updates current thinking in regard to operative developments contributing to myocardial protection, preconditioning, inhibition of adenosine triphosphate catabolism, the critical role of adenosine, management of myocardial edema, antioxidant therapy, endothelial cell injury, and the interaction between activated leukocytes and the endothelium. Some potential new directions for cardioprotection are identified.

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Superior myocardial preservation with modified UW solution after prolonged ischemia in the rat heart

Cited by 44 publications

References 12 publications

Comparative Study of Solutions for Pulmonary Preservation Using an Isolated Rabbit Lung Model

Comparative Study of Solutions for Pulmonary Preservation Using an Isolated Rabbit Lung Model

Prolonging myocardial preservation with a modified University of Wisconsin solution containing 2,3-butanedione monoxime and calcium

Future Cardioprotective Considerations

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