Superior low-immunogenicity of tilapia type I collagen based on unique secondary structure with single calcium binding motif over terrestrial mammals by inhibiting activation of DC intracellular Ca2+-mediated STIM1-Orai1/NF-кB pathway

Xu, Xiao Ming; Sui, Baiyan; Liu, Xin; Sun, Jiao

doi:10.1016/j.msec.2021.112503

Cited by 4 publications

(2 citation statements)

References 32 publications

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“…59 In the studies on tilapia collagen, it can be found that tilapia collagen has higher biocompatibility and lower immunogenicity than mammal collagen. 60,61 When compared with porcine collagen, tilapia collagen shows better activity in promoting bone-regeneration in both in vivo and in vitro studies. 4,62 The result of micro-CT above in this study provided further evidence that tilapia-derived-collagen-based scaffolds as bone graft materials may be a better alternative compared to traditional bovine-derived scaffolds.…”

Section: Resultsmentioning

confidence: 99%

A novel natural-derived tilapia skin collagen mineralized with hydroxyapatite as a potential bone-grafting scaffold

Yao

Shang

Ren³

et al. 2022

J Biomater Appl

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Collagen is widely used in medical field because of its excellent biocompatibility and bioactivity. To date, collagen for biomedical use is always derived from bovine or swine. The purpose of this study was to evaluate collagen-based biomaterials from non-mammalian donors for bone repair. Thus, tilapia skin collagen-hydroxyapatite (T-col/HAp) scaffolds were fabricated in three different proportions and then cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-N-hydroxysuccinimide (EDC-NHS). The scaffolds were evaluated for their microstructure, chemical and physical properties, mechanical strength and degradability. Then the in vitro responses of bone mesenchymal stem cells (BMSCs) to the scaffolds were investigated in terms of cellular proliferation, differentiation, and mineralization. At last, the scaffolds were implanted into rat skull critical defections to investigate the potential of osteogenic activities. As a result, the pore sizes and the porosities of the scaffolds were approximately 106.67–196.67 μm and 81.5%–66.7%. Pure collagen group showed a mechanical strength of 0.065 MPa, and the mechanical strength was significantly enhanced almost 17 times and 32 times in collagen/HAp ratio 1:4 and 1:9 groups. In vitro studies revealed the most prominent and healthy growth of BMSCs in collagen/HAp ratio 1:4 group. All the scaffolds showed certain osteogenic activities and those loaded with small amount of hydroxyapatite showed the strongest bioactivities. The micro-CT showed that the critical bone defect was almost filled with generated bone 6 months after implantation in collagen/HAp ratio 1:4 group. The biomechanics tests further confirmed the highest generated bone strength was in the collagen/HAp ratio 1:4 group. This study indicated aquatic collagen might be a potential alternative for type I collagen from mammals in bone tissue engineering. The combination of collagen and inorganic materials was also important and appropriate inorganic component loading can achieve both osteogenic quality and osteogenic efficiency to a certain extent.

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Section: Resultsmentioning

confidence: 99%

A novel natural-derived tilapia skin collagen mineralized with hydroxyapatite as a potential bone-grafting scaffold

Yao

Shang

Ren³

et al. 2022

J Biomater Appl

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“…For example, a contributing factor to the potent immunogenicity of the keyhole limpet hemocyanin (KLH), a nonheme protein used as a carrier protein in antibody production, − is its “foreignness”. Hemocyanin is found in arthropods and mollusks, with KLH being derived from the hemolymph of the giant keyhole limpet, a mollusk that is phylogenetically distant from humans. , Also, bovine serum albumin is nonimmunogenic in cows but elicits a strong immune response in mice and rabbits. ,− However, it is important to note that immune responsiveness being predicated on self-vs foreign contrast is overly simplistic because some evolutionarily conserved biomolecules, such as collagen, are weakly immunogenic in organisms where they are “foreign”. − On the other hand, spermatozoa are strongly immunogenic in the same organisms where they are “self”. − Nevertheless, designing biomaterials with biomolecules from phylogenetically distant organisms to the target organism is another principle to impart immunomodulatory properties.…”

Section: Design Principles For Immunomodulatorsmentioning

confidence: 99%

Design Principles for Immunomodulatory Biomaterials

Oluwole,

Weldu,

Jayaraman

et al. 2024

ACS Appl. Bio Mater.

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Fish Collagen Cross‐Linking Strategies to Improve Mechanical and Bioactive Capabilities for Tissue Engineering and Regenerative Medicine

Leonard,

Cumming,

Ali

et al. 2024

Adv Funct Materials

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Collagen is the most abundant protein found in humans and is fundamental to tissue structure and function. Collagen products used in biomedical research are primarily derived from mammals, and despite being mainly responsible for providing strength to native connective tissue, collagen hydrogels have comparatively low mechanical properties without the use of additional cross‐linking strategies. Alternative sources of collagen, like fish collagen, are emerging as key biomaterials in tissue engineering and regenerative medicine (TERM). By addressing cultural/religious concerns, ease of extraction, absence of mammalian‐derived allergens, and retention of functional motifs, fish collagen has many promising characteristics that make it a suitable alternative to mammalian collagen. Several physical and chemical cross‐linking strategies of fish collagen are explored to create more stable and resilient scaffolds for a variety of TERM applications. This comprehensive review explores how these modifications are optimized in fish collagen hydrogel systems. Herein, the use of fish collagen and their reported sources for TERM research, as well as the types of treatments (including sterilization) used to alter collagen structures and functions, are presented to date.

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Superior low-immunogenicity of tilapia type I collagen based on unique secondary structure with single calcium binding motif over terrestrial mammals by inhibiting activation of DC intracellular Ca2+-mediated STIM1-Orai1/NF-кB pathway

Cited by 4 publications

References 32 publications

A novel natural-derived tilapia skin collagen mineralized with hydroxyapatite as a potential bone-grafting scaffold

A novel natural-derived tilapia skin collagen mineralized with hydroxyapatite as a potential bone-grafting scaffold

Design Principles for Immunomodulatory Biomaterials

Fish Collagen Cross‐Linking Strategies to Improve Mechanical and Bioactive Capabilities for Tissue Engineering and Regenerative Medicine

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