Superior inorganic phosphate solubilization is linked to phylogeny within the Pseudomonas fluorescens complex

Browne, Patrick; Rice, Olivia; Miller, Simon H.; Burke, Jimmy; Dowling, David N.; Morrissey, John P.; O’Gara, Fergal

doi:10.1016/j.apsoil.2009.06.010

Cited by 97 publications

(45 citation statements)

References 44 publications

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“…Another subgroup harboring many efficient phosphatesolubilizing bacteria was subgroup 1g (with reference strain SBW25). There are other studies that have identified Pseudomonas strains closely related to SBW25 as strong P solubilizers (4,13). For future studies, it would be interesting to test strong phosphate solubilizers selected from subgroups 1d and 1g for efficacy in planta in comparison to strains of other phylogenetic backgrounds.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies on the occurrence, diversity, and evolution of plant-associated Pseudomonas spp. have focused mostly on the diversity of the 16S rRNA (4,31,33), other housekeeping genes (4,8,32), or biocontrol-relevant genes involved in the suppression of various plant pathogenic fungi (6,8,9,18,31,33,40). In addition, little is known about the occurrence of pqq genes among Pseudomonas species.…”

Section: Genes Pqqa -B -C -D -E -F -H -I -J -K and Pqqm (5)mentioning

confidence: 99%

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Pyrroloquinoline Quinone Biosynthesis GenepqqC, a Novel Molecular Marker for Studying the Phylogeny and Diversity of Phosphate-Solubilizing Pseudomonads

Meyer

Frapolli

Keel

et al. 2011

Appl Environ Microbiol

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Many root-colonizing pseudomonads are able to promote plant growth by increasing phosphate availability in soil through solubilization of poorly soluble rock phosphates. The major mechanism of phosphate solubilization by pseudomonads is the secretion of gluconic acid, which requires the enzyme glucose dehydrogenase and its cofactor pyrroloquinoline quinone (PQQ). The main aim of this study was to evaluate whether a PQQ biosynthetic gene is suitable to study the phylogeny of phosphate-solubilizing pseudomonads. To this end, two new primers, which specifically amplify the pqqC gene of the Pseudomonas genus, were designed. pqqC fragments were amplified and sequenced from a Pseudomonas strain collection and from a natural wheat rhizosphere population using cultivation-dependent and cultivation-independent approaches. Phylogenetic trees based on pqqC sequences were compared to trees obtained with the two concatenated housekeeping genes rpoD and gyrB. For both pqqC and rpoD-gyrB, similar main phylogenetic clusters were found. However, in the pqqC but not in the rpoD-gyrB tree, the group of fluorescent pseudomonads producing the antifungal compounds 2,4-diacetylphloroglucinol and pyoluteorin was located outside the Pseudomonas fluorescens group. pqqC sequences from isolated pseudomonads were differently distributed among the identified phylogenetic groups than pqqC sequences derived from the cultivation-independent approach. Comparing pqqC phylogeny and phosphate solubilization activity, we identified one phylogenetic group with high solubilization activity. In summary, we demonstrate that the gene pqqC is a novel molecular marker that can be used complementary to housekeeping genes for studying the diversity and evolution of plant-beneficial pseudomonads.

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Section: Discussionmentioning

confidence: 99%

Section: Genes Pqqa -B -C -D -E -F -H -I -J -K and Pqqm (5)mentioning

confidence: 99%

Pyrroloquinoline Quinone Biosynthesis GenepqqC, a Novel Molecular Marker for Studying the Phylogeny and Diversity of Phosphate-Solubilizing Pseudomonads

Meyer

Frapolli

Keel

et al. 2011

Appl Environ Microbiol

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“…Recent studies with Pseudomonas spp. isolated from soil suggests that capacity to solubilize P is associated with phylogenetic lineage and that it may be feasible therefore to select specific microorganisms or communities for enhanced capacity to mobilize P (Browne et al, 2009). Microbial activity and community composition in the rhizosphere is driven not only by availability of C, but also by interaction with various plant-and microbially derived signal molecules (Bais et al, 2006;Badri et al, 2009).…”

Section: Ecological Considerations and Future Prospectsmentioning

confidence: 99%

Soil Microorganisms Mediating Phosphorus Availability Update on Microbial Phosphorus

2011

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“…The experimental site, managed by Teagasc, is located at Knockbeg, County Carlow, Ireland (52°51'N, 6°56'W). Details concerning the agrichemical inputs and the geochemical properties of the soil have been described previously (Browne et al 2009, Chhabra et al 2012. The experiment design is a comparison between four replicate plots subjected to reduced (low) agricultural chemical inputs versus four subjected to conventional (high) inputs.…”

Section: Methodsmentioning

confidence: 99%

“…The objective of this study was to evaluate the complex influences of agrichemical input regimes and plant effects on microbial diversity, using a long-term agricultural site that has been running since 1994 (Browne et al 2009). The experimental monoculture system and its long period of stable chemical input levels make it a suitable model for studying the influences of these treatments on the bacterial community structure.…”

mentioning

confidence: 99%

Long-term agrichemical use leads to alterations in bacterial community diversity

Tan¹,

Barret²,

Rice³

et al. 2012

Plant Soil Environ.

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Bacterial communities are key drivers of soil fertility and agriculture productivity. Understanding how soil bacterial communities change in response to different conditions is an important aspect in the development of sustainable agriculture. There is a desire to reduce the current reliance on high inputs of chemicals and fertilisers in agriculture, but limited data are available on how this might impact soil bacterial communities. This study investigated the bacterial communities in a spring barley monoculture site subjected to two different input regimes for over 12 years: a conventional chemical/fertiliser regime, and a reduced input regime. A culture independent approach was performed to compare the bacterial communities through 16S rRNA gene PCR-DGGE. PCO analysis revealed that the rhizosphere has a strong structuring effect on the bacterial community. Moreover, high inputs of agrichemicals lead to an increase of phosphorus level in the soil and a concomitant reduction of the bacterial diversity. These results may help to evaluate the environmental risks associated with agrichemical usage.

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Superior inorganic phosphate solubilization is linked to phylogeny within the Pseudomonas fluorescens complex

Cited by 97 publications

References 44 publications

Pyrroloquinoline Quinone Biosynthesis GenepqqC, a Novel Molecular Marker for Studying the Phylogeny and Diversity of Phosphate-Solubilizing Pseudomonads

Pyrroloquinoline Quinone Biosynthesis GenepqqC, a Novel Molecular Marker for Studying the Phylogeny and Diversity of Phosphate-Solubilizing Pseudomonads

Soil Microorganisms Mediating Phosphorus Availability Update on Microbial Phosphorus

Long-term agrichemical use leads to alterations in bacterial community diversity

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